ISFop1

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022377 ND Francisella opportunistica
Francisella opportunistica
Francisella opportunistica PA05-1188
Francisella sp. PA05-1188
DNA section
IS Length : 1133 bp

Ends


IR Length : 12/15

IRL : CCAAACAAAAAACATTCTCATACTTATGTTATAATGTAATTAAGATAACT
IRR : CCAATCCAAATACATGATTAGGTAAACATCCATTTTCTAGAGCAGATAGA

Insertion site


Left flankDirect repeatRight flankDR Length
CAGAGCGTTTAATATAAGGTTTCTAAT2
ACAGTTGAGTTAtaTTACATAAACAAA2

DNA sequence

CCAAACAAAAAACATTCTCATACTTATGTTATAATGTAATTAAGATAACTATTTAATAATACCCATGCCTAGACCTATTAAGTTACCAGTAGATTTTGAC
AAGTACGATTATGCTGGCCTATCAAAAAAAGAGTCAAACCATAAAAACAAGATAAGACTGTTAGCAATGTCAAATATAAAAGATGGAATGAGTTTGCAAG
ATACTGGTAAAGTTTTAAAAACACCCTGGAAAACTATACAAACATGGCTACAGAACTTTAGAAAATATGGCATATCTAGCTTATATGTGAAAACCACAAA
ATATAAACCACCTAAAATAACGGAGGAAGTTAAGGTTTGGATTTCTAATTTCATGAAAACCTTATATAGTAATCAGGTTGGAGGTAGTATTACAGGTAAA
CAATTATTATGTTTAGTAAAACAGCATTTTGCTATAGAATGTTGTTTGCAAACTATATACAACACCCTACATAGCTTAAATCTTAGCTGGATAAGCTGTA
GATCTAAGCATCCAAGATCAGACATAGAAGTTCAAGAATTATATAAAAAAACTTTGAAAGTTATGTCAGAAAATTAATCCCAGAGAATGTTGATATATCT
ACTGTAGATATATGGGTTCAAGATGAGTCAAGAGTTGGTCAGCAAGGTAGCTTAACAAGAATATGGGCTCCAACGGGAACAAGACCACGCAAGGTTAAGC
AGGGACAGTTTATCTCTACATATATCTATGGTGCAGCATGTGCAAAGTCTAGAGATAGTTTTGGATTGATACTTCCAACTGCAAATACAATTGCTATGCA
AACCTACTTAAATCAATTATCAAATCATATTGCTGCTGGTCGACATATAGCTTTGGTTGTAGATAATGCAGGCTGGCACACCTCAAAGCAATTAGATATA
CCAAAAAATATTACTTTGATACCATTACCTCCATACTCTCCTGAACTTAACCCTATGGAGCAGGTATGGGAATGGATGAAAAATAACTATCTATCAAATA
TTTGCTTTGACAGTTACAATGACATTCTTGAAAAATTAACTATTGCATGGAATCATTTTTCATCTAATGCTGAATTGGTGAAATCTATCTGCTCTAGAAA
ATGGATGTTTACCTAATCATGTATTTGGATTGG
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
513 bp170 aa65577+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPRPIKLPVDFDKYDYAGLSKKESNHKNKIRLLAMSNIKDGMSLQDTGKVLKTPWKTIQTWLQNFRKYGISSLYVKTTKYKPPKITEEVKVWISNFMKTL
YSNQVGGSITGKQLLCLVKQHFAIECCLQTIYNTLHSLNLSWISCRSKHPRSDIEVQELYKKTLKVMSEN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
570 bp189 aa5471116+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KNFESYVRKLIPENVDISTVDIWVQDESRVGQQGSLTRIWAPTGTRPRKVKQGQFISTYIYGAACAKSRDSFGLILPTANTIAMQTYLNQLSNHIAAGRH
IALVVDNAGWHTSKQLDIPKNITLIPLPPYSPELNPMEQVWEWMKNNYLSNICFDSYNDILEKLTIAWNHFSSNAELVKSICSRKWMFT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1052 bp351 aa651116+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRPIKLPVDFDKYDYAGLSKKESNHKNKIRLLAMSNIKDGMSLQDTGKVLKTPWKTIQTWLQNFRKYGISSLYVKTTKYKPPKITEEVKVWISNFMKTL
YSNQVGGSITGKQLLCLVKQHFAIECCLQTIYNTLHSLNLSWISCRSKHPRSDIEVQELYKKKNFESYVRKLIPENVDISTVDIWVQDESRVGQQGSLTR
IWAPTGTRPRKVKQGQFISTYIYGAACAKSRDSFGLILPTANTIAMQTYLNQLSNHIAAGRHIALVVDNAGWHTSKQLDIPKNITLIPLPPYSPELNPME
QVWEWMKNNYLSNICFDSYNDILEKLTIAWNHFSSNAELVKSICSRKWMFT

 

Blast result :
Comments
ISFop1 is 59% aa similar to ISEcret5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References