ISfinder Mirror Site

ISPa103

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
MF344568	ND	Pseudomonas aeruginosa	Pseudomonas aeruginosa 727 Pseudomonas aeruginosa p727-IMP

DNA section

IS Length : 1381 bp

Ends

IR Length : 26/27

IRL : GATCGTGTCCCGGGAAGTGGTGTAACTGAACCAGCCGAAGGTGCCCTGCG
IRR : GATCGTGTCCCAGGAAGTGGTGTAACTCATCATGGCTCTGGCCACTGAGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCCTAACTCA	TCCCGGTGG	CGCTTGGTCG	9

DNA sequence

GATCGTGTCCCGGGAAGTGGTGTAACTGAACCAGCCGAAGGTGCCCTGCGGGCCAAAGAGGATGGTCATCGTGGTTCTTGGCTAACGTTGAGTTTGCGAA
GACCAAACACTCACCAGAGAAACCACGATGACCAAGCCCACTATCGCATTGACCGAGTTGGTTGAGAAAGGGGCAGACGCTGATCTGCTCAAGCAAATGA
TCCAGTTCGTTGCCCAGCGCATGATGGAGTTCGACGTCGAAGGCCTGTGCGGCGCGGGCTTCGATGTCAAAAGCCCAGACCGGACAAATAGCCGTAATGG
CTACCGCGATCGTCTTTGGCAAACCCGCGCAGGCGACGTCGACCTTAAGATTCCCAAGCTGCGCCAAGGCAGCTATTTCCCTGGTTTCCTTGAACCTCGA
CGCACCGCTGAGAAAGCCATGGCGGCAGTCATCCAGGAGGCCTACATCCAAGGCGTTTCAACGCGTTCGGTGGACGAGCTGGTCAAGGCCATGGGCATGA
CCGGTATCTCTAAAAGCCAGGTCTCACGGCTAGCCGGTGAGATCGATGAGCGCGTTCACGCCTTCCTTGACCGCCCGCTTGAGGGCGACTGGCCCTATCT
CTGGATCGATGCCACCTACGTCAAAGTGCGGGAGGCCGGACGCATCGTCTCGGTCGCCGTAATAATCGCCGTGGCCGTGAACACCGACGGTGGCCGCGAA
GTCCTGGGCATGCGGGTTGGGCCGTCAGAGGCTGAGCCGTTCTGGACAGACTTCCTGCGCAGCCTCATGCGACGTGGCTTGCGGGGCGTGAAGCTGGTCA
TCTCCGACGCCCACGAAGGCCTGAAAGCAGCTGTATCCAAGGTCTTCAATGCGACCTGGCAGCGCTGCCGTGTGCATTTCATGCGTAATGCCATGGCCCA
CGTCGGCAAAGGTCAGCGCACCATGGTGGCGGCTCTACTGCGCACGGTCTTCGCCCAGGACAGCCGTGCCGAATGCCATCAGCAATGGCGTTTGGTCGCA
GATCAGCTACGCGAAAAATACCCCAAGATCGCGGCGCTCATGGATGGTTGCGAGGATGAAGTGCTGGCGCACATGGCGTTTCCCAAGGCTCATCGACAGC
AGTTGCACAGCACCAACCCGCTTGAGCGGCTCAACGCTGAAATCAAACGCCGCACCGACGTCGTGGGGATTTTCCCCAACGATCCGGCCATTACGCGTCT
GGTAGGCGCGATGCTGCTGGAGCAGAACGACGAGTGGTGCCTGCAACGGCGTTACATGCAGTTGGAGGCCTTTGAGGCAGTCAGCGATAATCCACAGGCC
AAGCTGTCGGCCGTGATCAACTAAACGGCAAACTCAGTGGCCAGAGCCATGATGAGTTACACCACTTCCTGGGACACGATC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1197 bp	398 aa	128	1324	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKPTIALTELVEKGADADLLKQMIQFVAQRMMEFDVEGLCGAGFDVKSPDRTNSRNGYRDRLWQTRAGDVDLKIPKLRQGSYFPGFLEPRRTAEKAMAA
VIQEAYIQGVSTRSVDELVKAMGMTGISKSQVSRLAGEIDERVHAFLDRPLEGDWPYLWIDATYVKVREAGRIVSVAVIIAVAVNTDGGREVLGMRVGPS
EAEPFWTDFLRSLMRRGLRGVKLVISDAHEGLKAAVSKVFNATWQRCRVHFMRNAMAHVGKGQRTMVAALLRTVFAQDSRAECHQQWRLVADQLREKYPK
IAALMDGCEDEVLAHMAFPKAHRQQLHSTNPLERLNAEIKRRTDVVGIFPNDPAITRLVGAMLLEQNDEWCLQRRYMQLEAFEAVSDNPQAKLSAVIN

Blast result :

Comments

ISPa103 is 88% aa similar to ISCARN47.

References

1] Xiaoyuan Jiang (2019) Direct submission.
2] Yuan,M., Feng,J. 2nd, Zhan,Z. 3rd, Jiang,X. 4th, Zhang,D. 5th, Chen,X. 6th, Zhao,X., Che,J., Lu,J., Xu,J., Li,J. and Zhou,D. (2017) Direct GenBank submission.