ISfinder Mirror Site

ISPlge3

Family IS3
Group IS150

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP014777	ND	Pluralibacter gergoviae	Pluralibacter gergoviae Pluralibacter gergoviae strain FB2

DNA section

IS Length : 1442 bp

Ends

IR Length : 12/18

IRL : TGCACTGCACCCATTTAGTTGGACTCTCAAATTGGAGAGTCCCATGTCCA
IRR : TGAAAGCATCCCAGGTACAAATCAGCGGTTTAAATTCTCACTGGATAGTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATTTTCATTAACCCA		GGAGCACTCTTAAA	0

DNA sequence

TGCACTGCACCCATTTAGTTGGACTCTCAAATTGGAGAGTCCCATGTCCAGACGTAAGTATACCTTTGAACAGCGCCTCGAAGTGGTTATGCACTATTTC
GCTACTGATGAAGGCTATCGCTTAACATCCGCTCGCTTTAACGTTCCCAGAACCCAGGTAAGATTATGGGTTGCAGCCTATGATGCTTATGGAGAAGAAG
GTCTAAAGCCCAGAGATAAGGGCGTATCCATTGAACCCGAAATCAGAGTAGAAGCCGTGAAGGCTGTACTGACGGGAAGGATTTCCCAGACGCAGGCTGC
TGCGAAATTTAACGTCGCTGGTGCGGCCTCTGTTGGGAAATGGATGAAGGTGTTCAGCGAGCATGGAGAGGAAGGGCTTCGTTCTCTTCGTGTGGGCAAA
AAAAGGGCACTTCATATGATTGATGATCCTGTTACGCTTGAGGTAGCACTGGAACGTTCGAAAGATAAACGTATTCAGGAGCTTGAGCAAAAAGTACGGT
ATCTTGAGTTACGGGTGCTGTATCTAAAAAAGTTGAAAGCCTTAGTTCGGTAAGGGATAAGGTCAGAATTATCGACGAACTGAGGCAGCACTATCCGTTC
GACCAGCTTTTGCAGATAGCTCACATACCCAGAAGTACATTTTACTATCATCTGAAAGCGCTCCATTCCCCGGGGAAATACGATGAAGTAAAATGCAGAA
TCAAAGAAATTTATGATGAGAACCAGGGCCGATATGGTTATAGGCGGATAGCACTGGCGCTCAGAAGAGAGGGTGGGGCGTTGAACCACAAAGTGGTTCA
ACGCCTGATGAATTTACTACACCTCAAAGCCGCGATAAAAGTAAAACGCTACAGCTCCTGGCGGGGTGAGCATGGGCATATTGCTGACAATATCCTGAAA
CGAAACTTCAAAGCCAGCAGGCCCAATGAAAAATGGGTCACCGATGTCACTGAATTCGCCGTCAGTGGCCGAAAACTCTACCTTTCACCCATTATTGATC
TCTTCAATAACGAAGTGATTTCCTTCAGTATTTCAGAACGTCCAACCATGCCGATGATCGATGACATGCTCGTTAAGGCATTTGCCAGACTTGATGCGGG
CGTCAATCCGGTTCTTCATTCAGATCAGGGCTGGCAATACCGTCATCGATGGTACCAGTATCAGTTACGCGAGTTTGGCGTCATACAAAGTATGTCCCGC
AAAGGCAATTGCCTCGATAATGCCTGTGCAGAATGCTTTTTCGGGACGTTAAAATCAGAATGCTTTTACACCAGTGAGTTTAAGGATATTGAGGAACTAA
AGATCGCTATTGAGGACTACATACGATACTACAATACCCGGAGAATCAGCCTTAAATTTAATGGTCTTAGTCCGGTTGAATACCGCCTAAAGAACTATCC
AGTGAGAATTTAAACCGCTGATTTGTACCTGGGATGCTTTCA

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
510 bp	169 aa	44	553	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSRRKYTFEQRLEVVMHYFATDEGYRLTSARFNVPRTQVRLWVAAYDAYGEEGLKPRDKGVSIEPEIRVEAVKAVLTGRISQTQAAAKFNVAGAASVGKW
MKVFSEHGEEGLRSLRVGKKRALHMIDDPVTLEVALERSKDKRIQELEQKVRYLELRVLYLKKLKALVR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
906 bp	301 aa	508	1413	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VTGAVSKKVESLSSVRDKVRIIDELRQHYPFDQLLQIAHIPRSTFYYHLKALHSPGKYDEVKCRIKEIYDENQGRYGYRRIALALRREGGALNHKVVQRL
MNLLHLKAAIKVKRYSSWRGEHGHIADNILKRNFKASRPNEKWVTDVTEFAVSGRKLYLSPIIDLFNNEVISFSISERPTMPMIDDMLVKAFARLDAGVN
PVLHSDQGWQYRHRWYQYQLREFGVIQSMSRKGNCLDNACAECFFGTLKSECFYTSEFKDIEELKIAIEDYIRYYNTRRISLKFNGLSPVEYRLKNYPVR
I

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1370 bp	456 aa	44	1413	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRRKYTFEQRLEVVMHYFATDEGYRLTSARFNVPRTQVRLWVAAYDAYGEEGLKPRDKGVSIEPEIRVEAVKAVLTGRISQTQAAAKFNVAGAASVGKW
MKVFSEHGEEGLRSLRVGKKRALHMIDDPVTLEVALERSKDKRIQELEQKVRYLELRVLYLKKVESLSSVRDKVRIIDELRQHYPFDQLLQIAHIPRSTF
YYHLKALHSPGKYDEVKCRIKEIYDENQGRYGYRRIALALRREGGALNHKVVQRLMNLLHLKAAIKVKRYSSWRGEHGHIADNILKRNFKASRPNEKWVT
DVTEFAVSGRKLYLSPIIDLFNNEVISFSISERPTMPMIDDMLVKAFARLDAGVNPVLHSDQGWQYRHRWYQYQLREFGVIQSMSRKGNCLDNACAECFF
GTLKSECFYTSEFKDIEELKIAIEDYIRYYNTRRISLKFNGLSPVEYRLKNYPVRI

Blast result :

Comments

ISPlge3 is 80% (transposase) aa similar to ISKpn8. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPlge3 is disrupted by insertion of ISKpn49 (IS66 family). We reconstructed this copy of ISPlge3 by deletion of ISKpn49 and of one copy of the ISKpn49 DR's.

References

1] Zhe Zhan (2018) Direct submission.
2] Tan,J.-Y. and Chan,K.-G. (2016) Direct GenBank submission.