ISBdi14

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Bradyrhizobium diazoefficiens
Bradyrhizobium diazoefficiens SEMIA 5080
Bradyrhizobium diazoefficiens
Bradyrhizobium diazoefficiens USDA 122
DNA section
IS Length : 1044 bp

Ends


IR Length : 21/24

IRL : GAGCATGTTGTTTTTTACCGGAATCGGGATTCCCAGAGGGGGGAATTTCT
IRR : GAGCATGATGTTTTTATACGGAAACATAGCCTGAATTTTTGAGGTAGTTG

Insertion site


Left flankDirect repeatRight flankDR Length
AGCTCCTGCCTAGTCGGCAACT2

DNA sequence

GAGCATGTTGTTTTTTACCGGAATCGGGATTCCCAGAGGGGGGAATTTCTGATTCAAACTCCATGCTGGAGAACGGGGGCCAGCATGGATGACGCGACCC
TATTCGGAAGACCTTCGCGAACTCGCTCTGGCGAGGGCTGACGCAGGCGAGACGGTTCGTTCGATTGCAGAAGCGCTTCAGATCAGTCCTTCCTGTGTGA
CGAAGTGGAAGAATCTTCGGCGAGAGACCGGAGGCGTTTCTCCCGGCAAGATCGGCGGTCACAAGAAGCCGGTTCTGTCCGGCACCAACGCCGACTGGCT
GCGCAAACGCATTCGCTCCGGACCGTTCACTTTGCGGAAGCTGACGCAAGAACTGGCTGCGCGCGGAATCAAAACAGACGTGCGGGCGGTATGGACGTTC
GTTCACGCCGAGGGGCTGAGCTTCAAAAAAAACGCTCCGGCCGGCCGAACAAGATCGCCCCGATATCGCGCGTAAACGGACGCGCTGGAAGGCCCATCAG
GGCAGGATCGAGGCCTCACGCCTGGTCTTTATCGACGAGACCTGGATCAAAACCAACATGGCCCCACTGCGCGGCTGGGGACCGAGTGGGCAGCGTCTTG
AAGCATCTGCGCCTTTTGGCCACTGGAAGACCATGACCTTCATCGCCGCGCTGCGCCACGACCGGATCAGCGCGCCCTGGGTCATCGATGGCCCCATCAA
CGGTGAGCTCTTCACGCTCTACGTCGAGAAAGTTTTGGCGCCCACCTTGGCAAAAGGCGAGGTCGTCATTCTCGATAACCTGGGCAGTCATAAGGGCAAG
TCTGCCCGCAACGCCATCCGCGCCAGAGGGGCGCATCTCCTCTTCCTGCCGCCCTACAGCCCCGACCTCAATCCGATCGAGCAGGTCTTCGCCAAGCTCA
AACACCTGATGCGAGCCGCGCAGACACGTGATGTCGAAGCGACTTGGCGCAAGGTCGGCGAACTCCTCGATATCTTCTCCAAAGACGAATGCGCCAACTA
CCTCAAAAATTCAGGCTATGTTTCCGTATAAAAACATCATGCTC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
387 bp128 aa89475+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTRPYSEDLRELALARADAGETVRSIAEALQISPSCVTKWKNLRRETGGVSPGKIGGHKKPVLSGTNADWLRKRIRSGPFTLRKLTQELAARGIKTDVRA
VWTFVHAEGLSFKKNAPAGRTRSPRYRA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
612 bp203 aa4201031+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ASKKTLRPAEQDRPDIARKRTRWKAHQGRIEASRLVFIDETWIKTNMAPLRGWGPSGQRLEASAPFGHWKTMTFIAALRHDRISAPWVIDGPINGELFTL
YVEKVLAPTLAKGEVVILDNLGSHKGKSARNAIRARGAHLLFLPPYSPDLNPIEQVFAKLKHLMRAAQTRDVEATWRKVGELLDIFSKDECANYLKNSGY
VSV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
943 bp313 aa891031+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRPYSEDLRELALARADAGETVRSIAEALQISPSCVTKWKNLRRETGGVSPGKIGGHKKPVLSGTNADWLRKRIRSGPFTLRKLTQELAARGIKTDVRA
VWTFVHAEGLSFKKTLRPAEQDRPDIARKRTRWKAHQGRIEASRLVFIDETWIKTNMAPLRGWGPSGQRLEASAPFGHWKTMTFIAALRHDRISAPWVID
GPINGELFTLYVEKVLAPTLAKGEVVILDNLGSHKGKSARNAIRARGAHLLFLPPYSPDLNPIEQVFAKLKHLMRAAQTRDVEATWRKVGELLDIFSKDE
CANYLKNSGYVSV

 

Blast result :
Comments
ISBdi14 is 72% aa similar to ISGdi6.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Gesiele Carvalho (2018) Direct submission.