ISBdi7

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Bradyrhizobium diazoefficiens
Bradyrhizobium diazoefficiens SEMIA 5080
Bradyrhizobium diazoefficiens
Bradyrhizobium diazoefficiens USDA 122
DNA section
IS Length : 2479 bp

Ends


IR Length : 23/26

IRL : TGTCATTCGGCTTGCAATATTGACCCCCTAAGCCGGGGGATCGGCGTCCA
IRR : TGTCAATCGGCTTGCAAATTTGACCCTTCATCGGCGTCCAATTTTGACCC

Insertion site


Left flankDirect repeatRight flankDR Length
CCTTCGATGGGCTATGGCTC0

DNA sequence

TGTCATTCGGCTTGCAATATTGACCCCCTAAGCCGGGGGATCGGCGTCCAAAATTGCCCCCCTACAGGTTGGTCTGTTGCGCTGCCTGCTTCGTAACAAA
GCAGGTGGTGGAGGATGCTGGTCGTGGAGACGATTGCGCGGATTCGGCGCGAGCACTTCATCAAGGGCAAGACGATCAAGGAGATCGCCCGTGACCTGAA
GGTGTCACGGAACACGGTTCGGAAGGTGCTGAGGTCGGGAGAGACCTCCTTCGAGTATGAGCGGCAAGTGCAGCCGCGGCCAAAGCTCGGACGATGGGCA
GTCGAACTTGACGGGCTGCTTGCGGCGAACGCGGCTAAATCGGCTCGTGAGCAGCTGACATTGATCCGGATCTTCGAAGAGCTGCGCGGTCGCGGCTATG
ACGGCGGCTACGATGCGGTGCGACGTTACGCCAGGCGGTGGAGCAAGGAACGCGGGCAATCGACCGCGGCGGCCTATGTCCCGCTGAGTTTTGCGCCAGG
CGAAGCCTACCAGTTCGACTGGAGCCATGAGGTGGTGCTGTTGAGCGGAACCACCGTGGTCGTGAAGGTTGCTCACGTCCGGCTCTGCCACAGCCGTATG
CTATTTGCCCGGGCGTATCCGCGAGAGACGCAGGAGATGGTGTTCGACGCCCACGACCGAGCGTTCGCGCTGTTCAAGGGCACCTGCACCCGCGGCATCT
ACGACAACATGAAGACCGCCGTGGAGACGATCTTCGTCGGTAAAGGGCGTCTTTACAATCGCCGGTTTTTGCAGATGTGCAGCCACTATCTGGTCGATCC
AGTCGCCTGCACGCCGGCGTCTGGCTGGGAGAAGGGGCAGGTTGAGAACCAGGTCGGGCTGGTCAGGGAACGCTTCTTCACGCCGCGTCTGCGGTTCAAA
AACCTCGACGAGTTAAACGCCTGGCTGCTCGACAAATGCATCACCTACGCCAAGGCTCATCGCCATCCGGAACTGGTCGATCAGACGATCTGGGACGTGT
TCGAAGTCGAACGCCCTAGGCTCGTTCCCTATGCAGGGCGATTTGACGGCTTCCATGCGGTGACGGCGTCGGTCTCGAAGACCTGCCTGGTGCGCTTCGA
CAACAACAAGTACTCGGTCGCAGCCAGTGCAGTCGGACGACCGGTCGAGGTTCAAGCCTATGCCGATCGCATCGTGATCCGCCAGGATGGACGTATCGTT
GCCGAGCATCAGCGATCCTTTGGCCGCGGCGATACCGTCTACGACCCTTGGCATTATGTGCCGGTGCTCGCCCGCAACCCCGGCGCCTTACGCAACGGCG
CTCCCTTCAAGGACTGGGTGCTGCCGGCCGCGATCGAGCGGATCCGGCGCAAGCTTGCCAGCATCGACGATGGCAATCGGCAGATGGTCGACATCCTCAA
CGCGGTGCTGACTGACGGTCTGCCTGCGGTGGAAGCGGCCTGTGCCGAAGCGCTCAGTCACAGCGTTCATTCCGCCGATGTGGTGCTCAACATCCTGGCC
CGTCAACGTGAGCCAGCCCCGCCGGCCAACATCATGACGCCAGCCGCCCTGACGCTCCGTCATGCACCAATCGCCGATTGTGCCCGCTACGACAACCTTC
GGAGGACCAACTGATGGAACGAACTCAAATCTTCGACCTCATGGGCGAGCTCAAGCTCTATGGCATGAAGGCTGCCTTCGACGAGATCATGGCAACTGCC
GTCAAACGTCAGCATGAACCCCAGCGCATCGTTGGCGATCTACTCTCCGCCGAGATCAACGAGAAGCAAGCCAGATCTATCAAATACCAGCTCACCATTG
CCAAGCTGCCGCTTGCCAAGGACATTGCCGACTTCCAGTTCGACGGCACGCCGATCAATCAGACGCTCGTCAATGATCTTGCTGGCGGCGGCTTCGTCGC
CCAACAGCGCAACGTCGTGCTGGTCGGCGGCACCGGCACAGGCAAAACCCACCTGGCCATTGCCATCGCAAGAAGCTGCATCCGATCTGGTGCCCGCGGC
CGCTTCTTCAACGTGGTCGACCTCGTCAACCGCCTCGAGACCGAGACCCGCAATGGACGGCAGGGACGGCTTGCCGAGCATCTGACCCGGATGGACTTCA
TCGTGCTGGACGAACTCGGCTATTTGCCCTTCGCCCAGTCCGGTGGCCAGCTTCTCTTCCACCTCGTCAGCCGGCTCTATGAGCGCGCCTCCGTCATCGT
GACCACCAATCTCGCATTCGGCGAATGGCCCAGCGTGTTCGGCGACGCCAAAATGACCACAGCGTTGCTCGACCGATTGACCCATCACTGCGACATCGTC
GAGACCGGCAACGATAGCTGGCGGTTCAAAAGCCGAGACGACGATCACGCCACCCGCGCTCGTCTCGCCTCCGCTATCCCGGCCAGCTCCGACGAGACGA
GCGCTACCAGCAAACCCCGCCGCGGAAAGGGGTCAAAATTGGACGCCGATGAAGGGTCAAATTTGCAAGCCGATTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1500 bp499 aa1151614+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLVVETIARIRREHFIKGKTIKEIARDLKVSRNTVRKVLRSGETSFEYERQVQPRPKLGRWAVELDGLLAANAAKSAREQLTLIRIFEELRGRGYDGGYD
AVRRYARRWSKERGQSTAAAYVPLSFAPGEAYQFDWSHEVVLLSGTTVVVKVAHVRLCHSRMLFARAYPRETQEMVFDAHDRAFALFKGTCTRGIYDNMK
TAVETIFVGKGRLYNRRFLQMCSHYLVDPVACTPASGWEKGQVENQVGLVRERFFTPRLRFKNLDELNAWLLDKCITYAKAHRHPELVDQTIWDVFEVER
PRLVPYAGRFDGFHAVTASVSKTCLVRFDNNKYSVAASAVGRPVEVQAYADRIVIRQDGRIVAEHQRSFGRGDTVYDPWHYVPVLARNPGALRNGAPFKD
WVLPAAIERIRRKLASIDDGNRQMVDILNAVLTDGLPAVEAACAEALSHSVHSADVVLNILARQREPAPPANIMTPAALTLRHAPIADCARYDNLRRTN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
864 bp287 aa16142477+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MERTQIFDLMGELKLYGMKAAFDEIMATAVKRQHEPQRIVGDLLSAEINEKQARSIKYQLTIAKLPLAKDIADFQFDGTPINQTLVNDLAGGGFVAQQRN
VVLVGGTGTGKTHLAIAIARSCIRSGARGRFFNVVDLVNRLETETRNGRQGRLAEHLTRMDFIVLDELGYLPFAQSGGQLLFHLVSRLYERASVIVTTNL
AFGEWPSVFGDAKMTTALLDRLTHHCDIVETGNDSWRFKSRDDDHATRARLASAIPASSDETSATSKPRRGKGSKLDADEGSNLQAD

 

Blast result :
Comments
ISBdi7 is 86% aa (transposase) and 90% aa (helper of transposition) similar to ISMex39.
References
1] Gesiele Carvalho (2018) Direct submission.