ISKpn49

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP030859 ND Klebsiella Pneumoniae
Klebsiella Pneumoniae pQDE2-NR1
Klebsiella pneumoniae subsp. pneumoniae JNM8C2 plasmid pKJNM8C2.2
DNA section
IS Length : 2438 bp

Ends


IR Length : 21/22

IRL : GTAAGCGTACAGCGTGAACCGTCTGGTCATAATCTGAAGCATCCGACAAA
IRR : GTAAGCGTACAGCGAGAACCGTATTGACGGGGATGTGTTATTCAGTTGGC

Insertion site


Left flankDirect repeatRight flankDR Length
CAGTTTCTGAGGACAACAGA0

DNA sequence

GTAAGCGTACAGCGTGAACCGTCTGGTCATAATCTGAAGCATCCGACAAAGTGGTGTCCACCAAATAAGTAGTGGGAACCAAAGTGTCAGATATGCAGAA
AAATATGACTCCCGGCAGGCGTAAAGGCTGCCCTAATTATTCTCCTGAGTTTAAACAGCAACTCGTTGCTGCCTCCTGCGAACCCGGGATATCCATCTCA
AAACTGGCGCTTGAAAATGGCATTAATGCCAATCTGCTCTTTAAATGGCGCCAGCAGTGGCGCGAGGGAAAGCTGCTATTACCTTCCTCTGAGAGTCCTC
AGTTACTTCCTGTGACTCTCGATGCCACCGCCGTACAACCAGAACCGCCCGCTGAGGACTCAGAGCTCAGCATCAGCTGTGAGGTAACGTTCCGGCACGG
GACACTCCGCCTCAACGGCACTGTCAGCGAAAAGCTTCTGACTCTGCTGATACAGGAACTGAAGCGATGATCCCGCTACCAACAGGCACCAAAATCTGGC
TGGTTGCCGGTATTACCGATATGAGAAACGGCTTCAACGGGCTGGCTGCAAAAGTGCAGACGGCGCTGAAAGACGAACTGATGTCCGGCCATGTCTTCAT
CTTCCGGGGCCGCAGCGGCAGTCAGGTTAAACTTCTGTGGTCCACCGGCGACGGGCTGTGCCTGCTGACCAAACGGCTGGAGCGCGGGCGCTTCGCCTGG
CCGTCAGCTCGCGATGGCAAAGTGTTCCTCACACCGGCACAGCTGGCGATGCTCCTTGAAGGTATCGACTGGCGGCAGCCTAAAAGACTGCTTACGTCAC
TGACTATGTTGTAGGCCTCTTTATCCTGGTCGACGCTGAATGAGCCTGGTAATATATCCGGTATGAACAGCTCACGTCCTGACGATATCGATGAACTGAA
ACGTCTCCTTGCCGAACAGGAGGCGCTGAATCGTGCCCTGCAGGAAAAACTGCACGAGCGTGAACGCGAAATAGACCATCTGCAGGCGCAGCTGGATAAA
CTTCGCCGGATGAACTTCGGCAGCCGTTCCGAAAAAGTCTCCCGCCGTATCGCACAAATGGAAGCCGATCTGAACCGGCTTCAGAAAGAGAGCGATACGC
TGACCGGTCGGGTGGATGACCCGGCAGTACAGCGCACTCTGCGTCAGATCCGCACCCGCAAACCGTTCCCCGAATCACTCCCCCGCGATGAAAAAAGCCT
GCGGCCAGCGGGTTCCTGCTGCCCGGAGTGTGGTGGTGCGCTGAGTTACCTGGGTGAGGATGCCGCTGAACAGCTGGAGCTGATGCGCAGCGCCTTCCGG
GTGATCCGGACCGTACGGGAAAAACATGCCTGCACAAAATGTGATGCCATCGTGCAGGCCCCCGCGCCTTCGCGCCCCATTGAGCGGGGTATCGCCGGAC
CGGGGCTGATGGCCCGCGTGCTGACCTCAAAGTATGCAGAGCACACACCGCTGTATCGTCAGTCTGAAATATACGGTCGCCAGGGCGTGGAACTGAGCCG
TTCACTGCTGTCGGGCTGGGTGGATGCATGCTGCCGGCTGCTGTCCCCGCTGGAGGAGGCGCTTCAGGACTATGTCCTGACTGACGGTAAACTCCATGCT
GATGACACGCCTGTCCCGGTGCTGTTGCCGGGCAATAAGAAGACGAAGACCGGGCGGTTGTGGACGTATGTTCGTGATGACCGCAACGCCGGGTCAGCGC
TGGCACCGGCGGTGTGGTTCGCCTACAGCCCCGACAGAAAAGGCATCCATCCGCAGACCCATCTTGCGGGGTTCAGCGGTGTGCTACAGGCGGATGCGTA
CGCCGGGTTCAACGAGCTGTACCGCGATGGCCGGATAACGGAAGCCGCCTGCTGGGCTCACGCCCGCCGTAAAATCCACGATGTGCACGTTCGCACGCCG
TCAGCCCTGACGGAGGAAGCCCTGAAGCGTATCGGCGAACTGTATGCCGTGGAGGCGGAAATAAGAGGAATGCTGGCGGAACAACGGCTTGCTGAACGTC
AGCGGAAAACGAAACCGCTGCTTAGTACCCTGGAAAGCTGGTTGCGTGAAAAAATGAAAACGCTGTCGCGACACTCGGAGCTGGCAAAGGCGTTCACGTA
CACCCTGAACCAGTGGCCGGCCCTGACGTACTATGCAGAAAACGGCTGGGCCGAAGCCGATAATAACATCGCTGAAAATGCGCTGCGGATGGTCAGTCTG
GGTCGTAAAAACTGGTTGTTCTTCGGCTCAGACCACGGTGGTGAGCGGGGAGCGTTGCTGTACAGTCTGATCGGGACGTGCAAACTGAACGGCGTGGATC
CAGAAAGCTACCTTCGCCATGTCCTTGACGTCATAGCTGACTGGCCGGTCAACCGGGTCAGCGAGCTGCTACCCTGGCGCATCACACTGCCAACTGAATA
ACACATCCCCGTCAATACGGTTCTCGCTGTACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
378 bp125 aa93470+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MQKNMTPGRRKGCPNYSPEFKQQLVAASCEPGISISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDATAVQPEPPAEDSELSISCEVTF
RHGTLRLNGTVSEKLLTLLIQELKR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa467814+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPLPTGTKIWLVAGITDMRNGFNGLAAKVQTALKDELMSGHVFIFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGI
DWRQPKRLLTSLTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1539 bp512 aa8632401+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNSSRPDDIDELKRLLAEQEALNRALQEKLHEREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVDDPAVQRTLRQIRTRK
PFPESLPRDEKSLRPAGSCCPECGGALSYLGEDAAEQLELMRSAFRVIRTVREKHACTKCDAIVQAPAPSRPIERGIAGPGLMARVLTSKYAEHTPLYRQ
SEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTH
LAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAVEAEIRGMLAEQRLAERQRKTKPLLSTLESWLREKMKT
LSRHSELAKAFTYTLNQWPALTYYAENGWAEADNNIAENALRMVSLGRKNWLFFGSDHGGERGALLYSLIGTCKLNGVDPESYLRHVLDVIADWPVNRVS
ELLPWRITLPTE

 

Blast result :
Comments
ISKpn49 is 96% (transposase) aa similar to ISEc22.
References
1] Dongsheng Zhou (2018) Direct submission.
2] Bhattacharjee,B., Bardhan,T., Chakraborty,M. and Basu,M. (2018) Direct GenBank submission.