ISMae16
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP009552 | ND | Microcystis aeruginosa | Microcystis aeruginosa NIES-843 |
DNA section
IS Length : 1409 bp
Ends
Left end : ATTTACCTATAATGTCGGCTTGAAGGAACCCTCGGTTTCACCTCGGAGATGAAAAGTTCCGCCAGCATAGAACAGCGAAGCACGACTAAGAATAACTCTA II struct. : Yes
Right end : GGGGAGATTTAAGCTCTATTTTGGTTGGTAGAAATACCTGCTAAGACAACTTGAGTCAGTGAACCAAGAATCCCTCGCATAAGCGCGACGGGATTGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GGCATTAAGTGCGCTT | CCTT | CATTGACATTA | TCAA |
DNA sequence
ATTTACCTATAATGTCGGCTTGAAGGAACCCTCGGTTTCACCTCGGAGATGAAAAGTTCCGCCAGCATAGAACAGCGAAGCACGACTAAGAATAACTCTA
ATCTATAGTTGCCAATTTAACTTTAGTATGCTAGAGTAGTTTGCGTAGTAGGTCGATAACGCAAAATGTTTGTTTTAGAGTACAAGCTTAGAGGAAAACC
ATCTCAATATCAAGCCATTGATGAGGCTATTCGGACAGTACAGTTTGTTCGGAATAAATGTCTTAGATATTGGGAAGACAATAAAGGTGTAGGACAAAAG
GATGTATATAAATATGTCACTCAATTAAGAAGTGAATACCCTTTTGTTCAAGACCTTAACTCTACAGCTTGTCAACAGGCTTGCGAACGTACTTGGACTG
CTATTCTTAGGTTCTATAATAACTGTAAGAACAAAATTGCTGGTAAGAAAGGATATCCTAAATACTCTAAACGTACTCATTCTGTTGAGTTTAAAAAGTC
AGGTTGGAAACTTAATCGAAATTCCAAGAGAATAACTTTCACTGATGGAAAAAACATTGGGGAGTTAAAACTAATTGGTAGTCGAGACTTGTTTGATTTT
CAAGAATGGCAAATTCAACGGATAAGAATAGTTAAAAGGGCTGATGGATATTATTGCCAGTTAATGCTAAAACTCGATGCTAGAGATATAACCCCACAAT
TAGAATCGACGGGTCATTGCCTAGGATTAGATATGGGGTTAAAATATCTTTATGCTGACAGTAACGGGAATACAGTAGAGCCTCCTAAGTATTATCGAAA
AGCCGAGAAACGTCTTCATAAACTGAATAGAAGAAAGTCGAAAAAGTTTAAGAGAGGACAAAAGCAGTCTAATAACTACAAAAAAGCTAGTCAAAAATAC
GCCAAGGGACATTTAAAAGTAAGTAGGCAACGAGAAGAGTTTGCTAAAAAATTGGCACTCCGTTTAATTCAGTCAAACGACTTGATAGCCTACGAGGATT
TAAAAGTCAAGAACCTTGTGCGTAATCGAAAACTAGCTAAGAGTATCAATGATGCTGGTTGGTCACAATTGAGAAAATGGATAGAGTATTTTGGCATTAA
ATACGGCCGATTAACCATAGCAGTTAATCCAGCCTATACAAGTCAAGAATGCTTTAATTGTGGTCGGTTAATCCAAAAGTCTCTATCAGTTAGAACTCAT
GTTTGTTCCTGCGGATATGTGGAAGATAGAGACAAAATGGCGGCCTTGAATATACTCAAAAAAGCTACGATAGGGCATATCGGAAGCTGGTCGTTAGACC
TAAACGCTTGGGGAGATTTAAGCTCTATTTTGGTTGGTAGAAATACCTGCTAAGACAACTTGAGTCAGTGAACCAAGAATCCCTCGCATAAGCGCGACGG
GATTGTCAA
ATCTATAGTTGCCAATTTAACTTTAGTATGCTAGAGTAGTTTGCGTAGTAGGTCGATAACGCAAAATGTTTGTTTTAGAGTACAAGCTTAGAGGAAAACC
ATCTCAATATCAAGCCATTGATGAGGCTATTCGGACAGTACAGTTTGTTCGGAATAAATGTCTTAGATATTGGGAAGACAATAAAGGTGTAGGACAAAAG
GATGTATATAAATATGTCACTCAATTAAGAAGTGAATACCCTTTTGTTCAAGACCTTAACTCTACAGCTTGTCAACAGGCTTGCGAACGTACTTGGACTG
CTATTCTTAGGTTCTATAATAACTGTAAGAACAAAATTGCTGGTAAGAAAGGATATCCTAAATACTCTAAACGTACTCATTCTGTTGAGTTTAAAAAGTC
AGGTTGGAAACTTAATCGAAATTCCAAGAGAATAACTTTCACTGATGGAAAAAACATTGGGGAGTTAAAACTAATTGGTAGTCGAGACTTGTTTGATTTT
CAAGAATGGCAAATTCAACGGATAAGAATAGTTAAAAGGGCTGATGGATATTATTGCCAGTTAATGCTAAAACTCGATGCTAGAGATATAACCCCACAAT
TAGAATCGACGGGTCATTGCCTAGGATTAGATATGGGGTTAAAATATCTTTATGCTGACAGTAACGGGAATACAGTAGAGCCTCCTAAGTATTATCGAAA
AGCCGAGAAACGTCTTCATAAACTGAATAGAAGAAAGTCGAAAAAGTTTAAGAGAGGACAAAAGCAGTCTAATAACTACAAAAAAGCTAGTCAAAAATAC
GCCAAGGGACATTTAAAAGTAAGTAGGCAACGAGAAGAGTTTGCTAAAAAATTGGCACTCCGTTTAATTCAGTCAAACGACTTGATAGCCTACGAGGATT
TAAAAGTCAAGAACCTTGTGCGTAATCGAAAACTAGCTAAGAGTATCAATGATGCTGGTTGGTCACAATTGAGAAAATGGATAGAGTATTTTGGCATTAA
ATACGGCCGATTAACCATAGCAGTTAATCCAGCCTATACAAGTCAAGAATGCTTTAATTGTGGTCGGTTAATCCAAAAGTCTCTATCAGTTAGAACTCAT
GTTTGTTCCTGCGGATATGTGGAAGATAGAGACAAAATGGCGGCCTTGAATATACTCAAAAAAGCTACGATAGGGCATATCGGAAGCTGGTCGTTAGACC
TAAACGCTTGGGGAGATTTAAGCTCTATTTTGGTTGGTAGAAATACCTGCTAAGACAACTTGAGTCAGTGAACCAAGAATCCCTCGCATAAGCGCGACGG
GATTGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1188 bp | 395 aa | 166 | 1353 | + | No |
AG : TnpB
ORF sequence :
MFVLEYKLRGKPSQYQAIDEAIRTVQFVRNKCLRYWEDNKGVGQKDVYKYVTQLRSEYPFVQDLNSTACQQACERTWTAILRFYNNCKNKIAGKKGYPKY
SKRTHSVEFKKSGWKLNRNSKRITFTDGKNIGELKLIGSRDLFDFQEWQIQRIRIVKRADGYYCQLMLKLDARDITPQLESTGHCLGLDMGLKYLYADSN
GNTVEPPKYYRKAEKRLHKLNRRKSKKFKRGQKQSNNYKKASQKYAKGHLKVSRQREEFAKKLALRLIQSNDLIAYEDLKVKNLVRNRKLAKSINDAGWS
QLRKWIEYFGIKYGRLTIAVNPAYTSQECFNCGRLIQKSLSVRTHVCSCGYVEDRDKMAALNILKKATIGHIGSWSLDLNAWGDLSSILVGRNTC
SKRTHSVEFKKSGWKLNRNSKRITFTDGKNIGELKLIGSRDLFDFQEWQIQRIRIVKRADGYYCQLMLKLDARDITPQLESTGHCLGLDMGLKYLYADSN
GNTVEPPKYYRKAEKRLHKLNRRKSKKFKRGQKQSNNYKKASQKYAKGHLKVSRQREEFAKKLALRLIQSNDLIAYEDLKVKNLVRNRKLAKSINDAGWS
QLRKWIEYFGIKYGRLTIAVNPAYTSQECFNCGRLIQKSLSVRTHVCSCGYVEDRDKMAALNILKKATIGHIGSWSLDLNAWGDLSSILVGRNTC
Blast result :
Comments
ISMae16 is 70% (TnpB) aa similar to ISAsp18.
References
1] ISfinder submission (2018)
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.