ISfinder Mirror Site

ISMae15

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AP009552	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1635 bp

Ends

Left end : CGAAAATTTGGGTTAAAACCCCGTCGTTTTACGACGGCTTTTCCTGATTTTTAATGTAGCACTTAAGAACTTCCAGTGGCGCACCTCTCAGTTATCAGTT II struct. : Yes
Right end : AGCAGCATCTCGTGAAGCGTCAACCCAGCGGTCGGTCGTTCAATTAAGTTTGTTTGATCTGCCGGTCATCACCGTCCGGCCCCGGCGGTGAGGATGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
AGTACCCAAC	TTAT	AATACAGAAG	TCAA

DNA sequence

CGAAAATTTGGGTTAAAACCCCGTCGTTTTACGACGGCTTTTCCTGATTTTTAATGTAGCACTTAAGAACTTCCAGTGGCGCACCTCTCAGTTATCAGTT
ATCAGTTATCAGTTATCAGTGGGTAAGTTATCAGTTATCAGATGTGAGTTTTTAAGTGTGCAGTATTAAATAAGTAGTCGTGCAAAATTAATTTCCTAGT
GAAGATAGGCAAGAGACAAGAGACAAGAGGCAAGAGGTGGTTAGATATGTGTAATTAATTTTGCTTAGGTACTTAGAAGTTTCCTACTGTCTTTTTACTG
ATTACTGATCACTGATTACTAACCGACTTGGCATTATTGACAGACCTAGTTAGAATAGAGTACAGACACAAATATCTTAACCCAATGAAAGCGAGGTATC
AATACCGTATTTACCCAACAGACCAACAAAAGAGGCTTTTGTCTCAATTGTTCGGGTGTGTGCGCATTGTCTGGAACGATACCTTAGCTTACTGTCAAGA
ACTCTATCGACAAGGGGAGAAAAAGCCAAAATATACCGAGTTATCTAAAAGACTAACTCAAATTAAAAAGACGACAGAAAAAGTCTGGCTGACCGAGGTT
TCTTCTATCCCTTTACAACAGTCTTTGAGAGACTTAGAGACAGCCTATTCTAACTTTTTTGCATCTTGCAAGGGAGAGAGAAAAGGAAGAAAAGTAAAAC
CTCCTAAATTTAAGAAGCGTAAATCTAAACAATCAGCGAGATTTACTGACAATGGTTTTACCATCAATCAATATCACGTTACTTTAGCCAAAATCGGTGA
TTTAAGAATCGTTTGGAGTCGCCCATTACCCTCTAAACCTTCTAGCGTTACCGTGATTAAAGATGCAGCAGATCGCTATTTTCTCAGCTTTGTTGTCGAG
ATTCAGCCCGAAATACTTCCTAATAACGGGGAGTCCGTGGGAATCGATCTAGGGATTGCTACTTTTGCGACTCTCTCAACAGGGGAAAAGATAGACGCAC
CGAAACCGTTAAAGAAACAATTAAAACGGCTAAGAAAGGCACAGAAAAACCTTTCTAGAAAGCAAAAAGGAAGTAACCGACGGGAAAAAGCTAGGAAACG
AGTAGCTAAAATCCACGCAAAGATTAAGGACACTCGTACTGATTTCTTGCAGAAACTATCCACTAGAGTTGTTCGTGAAAATCAAACGATAATTTTAGAG
GATTTAAACACATCGGGAATGGTTAAAAATCGTAAGTTATCCCGTGCCATATCAGACTTAGGATGGCGTTCTTTCCGAGATATGCTATCGGCAAAATCTG
ATAAATATGGGCGTAATTTTCGGATAATTTCCCGATGGGAGCCAACGTCTCAGCGATGTTCCTGTTGTGGGAATATTGGCGGGAAAAAAGCGTTAAATAT
CCGTGAGTGGGAATGTCTTTTCTGTGGGACTTTTCATGATCGAGACGTGAACGCCGCAATTAATATCAAGGTCGCCGGTGGGCAATCGGAGACTTTAAAC
GGGCGTGGAGGACAGCATAAGACTTCTGTTAAAGAAGCAGCATCTCGTGAAGCGTCAACCCAGCGGTCGGTCGTTCAATTAAGTTTGTTTGATCTGCCGG
TCATCACCGTCCGGCCCCGGCGGTGAGGATGTCAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1242 bp	413 aa	385	1626	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKARYQYRIYPTDQQKRLLSQLFGCVRIVWNDTLAYCQELYRQGEKKPKYTELSKRLTQIKKTTEKVWLTEVSSIPLQQSLRDLETAYSNFFASCKGERK
GRKVKPPKFKKRKSKQSARFTDNGFTINQYHVTLAKIGDLRIVWSRPLPSKPSSVTVIKDAADRYFLSFVVEIQPEILPNNGESVGIDLGIATFATLSTG
EKIDAPKPLKKQLKRLRKAQKNLSRKQKGSNRREKARKRVAKIHAKIKDTRTDFLQKLSTRVVRENQTIILEDLNTSGMVKNRKLSRAISDLGWRSFRDM
LSAKSDKYGRNFRIISRWEPTSQRCSCCGNIGGKKALNIREWECLFCGTFHDRDVNAAINIKVAGGQSETLNGRGGQHKTSVKEAASREASTQRSVVQLS
LFDLPVITVRPRR

Blast result :

Comments

ISMae15 is 74% aa similar to ISTel2.

References

1] ISfinder submission (2018)
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.