ISfinder Mirror Site

ISMae13

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AP009552	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1427 bp

Ends

Left end : ATCTTGAATGTCGGGGTGAAGACCCTCGCTTTTAGCGACGGGATGAAACCCCGACCAGTATAAAACAGCGAAGCACGACATTTCGACAGGCTCAATGTAA II struct. : Yes
Right end : TTGGGGAGAGATTCCCTCTGGTTTGGTTGGATACGTCCTGCCAGATTAAGGAAACTCAGTGAACCAAGAATCCCTCGCTTAAGCGCGATGGGAGTGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GGACATTTAGGCTATG	TTTT	TGTTGTGTCCTACTGC	TCAA

DNA sequence

ATCTTGAATGTCGGGGTGAAGACCCTCGCTTTTAGCGACGGGATGAAACCCCGACCAGTATAAAACAGCGAAGCACGACATTTCGACAGGCTCAATGTAA
AAGCTCAATGTTCACTAAAATCTTGACAATCCGATGCTCTACAGGTTAACTTAAAATAAGTTGTTTGAGGTCGATAAACAATGATTGTCTTAGAAATGAA
AGCCGTGGTTAAACCAAGTCAGTGTTCTGCCATTGATGAAGCTATTCGTACAGTACAATTCATCCGTAATAAGGCTTTAAGGCTTTGGATGGATGCCAAG
AGAGAAGACAAAATCGATAAATATTCTCTCAATAAATATTGTGCAGTTCTCGCTAAACAGTTCAAGTTTGTCGATACTCTAAATTCTACTGCTAGACAAG
CATCGGCCGAACGAGCTTGGTCAGCTATTGCTCGTTTCTATGACAATTGTAAGAAAAAGGTTAAAGGTAAAAAGGGTTATCCCAAGTTTCAGAAAAATAA
TCGTTCTGTGGAATATAAAAACTCCGGATGGAAATTATCGGAGGATAGAAAGAAAATCACTTTCACAGATAAGAAAAACATTGGCACGGTTAAACTAAAA
GGAACCAGAGACCTAAACTTTTATCCTATTGACCAAATTAAACGAGTTAGGATTGTTAAACGAGCAGATGGTTATTATGTCCAATTCTGTCTCAGCGTTG
ATATTCGGGAATATGCGAAACCGCTAGAACCGACTAAAAGATGTGTGGGATTGGATGTAGGTTTAAAAGTTTTCTACGCTAATAGTGATGGGGAAACGGT
AGAAATACCGCAATACTATCAACCGGCAGAAAAAAGATTAAACCGACTGAATCGGAAGAAATCTAAAAAGTTTAGAAAAGGTCAACCCCAATCAAACAAC
TACCAAAAAGCTAGAAAGAGATATGCTAGAAAGCATTTAAGAGTAAGTAGGCAACGTAAAGGCTTTGTCGAAAAAGAGGCATTGCGCGTCATTAAATCTA
ACGATTTCATCGCTTACGAAAACTTAAATATTCAAGGCATGGTAAAAAACTCTAGACTAGCTAAATCTATCAATGATGTGGCTTGGTCAACTTTCCGACA
ATGGTTAGAATATTTTGGTTTTAAATATGGTAAGGCTACAGTAGCAGTAGCCCCCCATAACACGAGTCAAAATTGCTCTAATTGTGGTCAAAAAGTCCCT
AAATCTCTATCTACAAGAACCCATATTTGTCCTGACTGTGGCTATGTAGAAGATAGAGATATTAACGCCGCTATCAATATTCTCAAAAAGGGACTAAGTA
CCGTGGGACACACGGAAACTAATACGCTTGGGGAGAGATTCCCTCTGGTTTGGTTGGATACGTCCTGCCAGATTAAGGAAACTCAGTGAACCAAGAATCC
CTCGCTTAAGCGCGATGGGAGTGTCAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1209 bp	402 aa	181	1389	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MIVLEMKAVVKPSQCSAIDEAIRTVQFIRNKALRLWMDAKREDKIDKYSLNKYCAVLAKQFKFVDTLNSTARQASAERAWSAIARFYDNCKKKVKGKKGY
PKFQKNNRSVEYKNSGWKLSEDRKKITFTDKKNIGTVKLKGTRDLNFYPIDQIKRVRIVKRADGYYVQFCLSVDIREYAKPLEPTKRCVGLDVGLKVFYA
NSDGETVEIPQYYQPAEKRLNRLNRKKSKKFRKGQPQSNNYQKARKRYARKHLRVSRQRKGFVEKEALRVIKSNDFIAYENLNIQGMVKNSRLAKSINDV
AWSTFRQWLEYFGFKYGKATVAVAPHNTSQNCSNCGQKVPKSLSTRTHICPDCGYVEDRDINAAINILKKGLSTVGHTETNTLGERFPLVWLDTSCQIKE
TQ

Blast result :

Comments

ISMae13 is 79% aa similar to ISTosp1.

References

1] ISfinder submission (2018)
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.