ISRein1

  • Family IS5
  • Group ISL2
MGE type ISRelated element(s) :
Isoform Synonym(s) ISRin1
Accession numberTranspositionOriginHost
GL379592 ND Regiella insecticola
Regiella insecticola LSR1
DNA section
IS Length : 920 bp

Ends


IR Length : 30/34

IRL : TATTAGACCTCTTCGGAAACTAGCATTCATATAAGAATTTTGGTAAAACA
IRR : TAATAGACCTCTTCGGAAACTATCATTTATCTAATTTCCATATTATAAAT

Insertion site


Left flankDirect repeatRight flankDR Length
GCCGACTTTGTTTATTCTTCATC3

DNA sequence

TATTAGACCTCTTCGGAAACTAGCATTCATATAAGAATTTTGGTAAAACACTCCTGAACAAGGAGCCGCCATGAAATATGAAGTAATAAAAGTACTGGAA
GAGGAAAAATTTCGTCGCTTAACGGGTGTTAAACGCAGTACATTTGAAAAGATGATAAAGATATTGAATGAAGCAGATAAGAGTAAGCAAGGGAAAGGAG
GTCGGAAACCCAAGCGGGGTTTAGAAGAGCGCTTATTAATGGCACTGGAGTATATACGAGAATATCGCACTTATTTTCATGTCAGCCAAAGCTATGGCGT
GAGTGAGAGCACATGCTATGAAACGATTAAATGGATAGAGAATACGCTAATAAAGCATCCTGATTTTGCCCTACCTGGACGCAAAGCTGTATTAAAAAGG
GATAGGGAGTATGAGCTGGTTCTTATTGATGCCACAGAAACGCCGATTGAACGTCCCCAAAAAAACAAAAGCCGTTTTACTCAGGAAAAAAGAAACGACA
CACCTTAAAAACACAAGTGCTCGTTGATAAAAAAAGTAAAGCAGTCATCTGTACAAGCTTTACGAATGGGAGGCGCCATGATTTTAGGTTGTTTAAGGAG
TCTGGTGTGCGAATACATCCTGACATCAAAACAGTTACCGATACAGGTTACCAAGGGCTTGGCAAGATTCATTCAAACTCGGCGTTGCCTAAGAAAAAGA
CAAAGAAAAATCCCTTAACAAAAGAAGATAAACGCAACAATCGTGCGTTATCAAGCCAGCGTGTATTAAACGAACATGTCATTGGTATGATTAAAAGATT
TAAAATTGTATCAGACCGTTATCGAAACAGGCGAAAACGTTTTGGATTAAGATTTAATCTGATTGCTGCAATTTATAATATGGAAATTAGATAAATGATA
GTTTCCGAAGAGGTCTATTA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
438 bp145 aa71508+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKYEVIKVLEEEKFRRLTGVKRSTFEKMIKILNEADKSKQGKGGRKPKRGLEERLLMALEYIREYRTYFHVSQSYGVSESTCYETIKWIENTLIKHPDFA
LPGRKAVLKRDREYELVLIDATETPIERPQKNKSRFTQEKRNDTP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
444 bp147 aa451894+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TSPKKQKPFYSGKKKRHTLKTQVLVDKKSKAVICTSFTNGRRHDFRLFKESGVRIHPDIKTVTDTGYQGLGKIHSNSALPKKKTKKNPLTKEDKRNNRAL
SSQRVLNEHVIGMIKRFKIVSDRYRNRRKRFGLRFNLIAAIYNMEIR

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
824 bp274 aa71894+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKYEVIKVLEEEKFRRLTGVKRSTFEKMIKILNEADKSKQGKGGRKPKRGLEERLLMALEYIREYRTYFHVSQSYGVSESTCYETIKWIENTLIKHPDFA
LPGRKAVLKRDREYELVLIDATETPIERPQKKQKPFYSGKKKRHTLKTQVLVDKKSKAVICTSFTNGRRHDFRLFKESGVRIHPDIKTVTDTGYQGLGKI
HSNSALPKKKTKKNPLTKEDKRNNRALSSQRVLNEHVIGMIKRFKIVSDRYRNRRKRFGLRFNLIAAIYNMEIR

 

Blast result :
Comments
ISRein1 is 87% aa similar to ISOt6.
The original sequence was submitted to NCBI under the name ISRin1 but this is not the correct nomenclature.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2018)
2] Degnan,P.H., Gibbs,R.A., Richards,S. and Moran,N.A. (2009) Direct GenBank submission.