ISKpn33

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP002474 ND Klebsiella pneumoniae
Klebsiella pneumoniae U-0608239 plasmid pUUH239.2
DNA section
IS Length : 2526 bp

Ends


IR Length : 16/21

IRL : GTAAGCGTCAAGCCACTGCCGCCTGTGCTTCTGGTCCCGGGATCGCTAGC
IRR : GTAAGCGTTAAGTGAGCGCCGTATTGACGGTTATTTATCGGTAAGAACCA

Insertion site


Left flankDirect repeatRight flankDR Length
GTTTCATGTGTATGGAAGTCCGCTTTATCACA8

DNA sequence

GTAAGCGTCAAGCCACTGCCGCCTGTGCTTCTGGTCCCGGGATCGCTAGCTTAGAGCTCCGTCTAATTTAGAAGGAGCTCTGTTTATGGAAAATGCTGCC
AACTGGCGAACTGAATCGCGTACCGTCTATTCCAATGACTTCAAACTTCGGATGGTCGAACTGGCTTCACGACCAGATGCCAACGTCGCGCAACTGGCGC
GGGAACATGGCGTTGATAATAATCTCATTTTTAAGTGGCTACGCCTCTGGCAGAGAGAGGGGCGAATCTCTCGTCGAATGCCTGCAACTATCGTGGGGCC
GGTGGTACCACAACCTCTTCCGGTTTCCCCGACTCTGTTGTCCGTCGACGTAATCAACGACCCGCAGCCCATTGCAGAGAATGACACTCTGTGTACGTTC
TCCTCCACTCACTCCAGCGCGACTTCCTGTCATGTTGAGTTCCGCCACGGCAAAATGACGCTGGAAAACCCGTCGTCAGAGTTGCTGGCCGTGCTGATCC
GCGAACTGACCGGGAGAACACAATGATATCCCTCCCGTCAGGCACCCGCATCTGGCTGGTCGCTGGTGCCACGGATATGCGTAAATCCTTCAACGGGCTG
GGCGAACAGATACAACACGTTCTGGATGAGGCTCCCTTCTCCGGCCACCTGTTCATCTTCCGGGGACGCCGTGGCGATACCGTGAAAATACTCTGGGCTG
ATGCTGACGGTCTGTGCCTGTTTATCAAACGTCTGGAAGAAGGCCAGTTCGTCTGGCCAACAGTCCGTGATGGCAAAATCGCGATCACCCGCTCACAACT
TGCCATGCTTCTCGATAAGCTGGACTGGCGTCAGCCAAAAACAGCACGCCTTAACTCACTGACAATGTTGTAAAAAGAGCATGATCGCATTATAAATGGG
GTCATGAGTCAGGACTATCCCGCCCGTATCGCTGCGCTGGAAGACGCGCTTCGCCAGAAAGACAGTCAGCTCAGTCTCATTGCAGAGACTGAGTCGTTCC
TGCGTTCGGCGCTGTCCCGCGCTGAAGAGAAAATAGAGAACGAAGAGCGCGAAATAGAGCATCTGCGGGCATAGATAGAAAAACTGCGGCGGATGTTGTT
CGGTACCCGTTCTGAAAAGCTGCGCCGGCAGGTTGAAGAAGCAGAGGCCCTGCTGAAGTTGCAGGAGCAGCAAAGCGATCGTTACAACGGTCGGGATAAC
GATCAACAGGTACCCCGCCAGCTGCGCCAGTCCCGTCACCGTCGTCCTCTCCCGGAACATCTTCCCCGCGAGATCCATCGCCTGGAGCCTGTTGAACGCT
GCTGCCCGGACTGCGGCAGTGATATGAAATACCTCAGTGAAGTCAGCGCGGAACAGCTGGAACTGGTCTCCAGCGCCCTGAAAGTGATCCGCACGGTCAG
GGTGAAAAAGGCCTGTACCCGATGCGACTGCATCGTTGAAGCTCCTGCGCCATCACGCCCCATCGACCGGGGTATCGCCGGGCCGGGTCTGCTGGCCCGC
GTGTTAACGGCCAAATACTGTGAGCACACCCCGTTATACCGGCAGACAGAAATCCTTGCGCGTCAGGGCGTGGAGCTGAGCCGGGCACTGCTCTCTAACT
GGGTGGATGCCTGTTGCCGGTTAATGGCACCGCTGGATGATGCCCTTTATCACTACGTGATGGACTGCCGCAAACTGCATACGGATGATACCCCGGTGCC
AGTTCTGGCACCGGGCAGAAAGAAGACGAAAACCGGGCGCATCTGGACGTATGTTCGCGATGACCGGAATGTCGGCTCGTCAGACCCGCCCGCAGCATGG
TTCGCCTTCTCGCCGGACCGGCAGGGGAAGCATCCTCAGCAACATCTTCGCTATTATCATGGTGTGCTGCAGGCGGATGCCTTCGCAGGCTACGACCGGT
TGTTCAGCGCAGAACGTGACGGCGGCCCGTTAACCGAAGCGGCCTGCTGGGCCCACGCGCGCCGAAAAATCCACGACGTCTATATAAGCACTCATACAGC
AACAGCAGAGGAAGCCCTGAAACGTATCGGCGAGCTGTACGCAATAGAAGAAGCAATACGGGGCCTCCCTGCAACTGAGCGGCTGGCAGCCAGGCAGTCC
CGAAGTAAGCCGCTGCTGATATCCCTGCATGACTGGTTGGTGGAGAAAAGCGCCACTCTGTCGAAAAAATCCCGTCTGGGCGAAGCGTTCGCTTATGCCC
TGAATCAGTGGGATGCGCTGTGCTACTACTGTGATGATGGCCTGGCAGAGCCTGATAACAACGCAGCCGAACGAGCTCTTCGTGCCGTCTGTCTTGGGAA
AAAGAATTTTATCTTCTTCGGCAGCGACCACGGCGGTGAGCGCGGAGCCCTGCTGTATGGTCTTATCGGAACATGCAGGCTCAATGGTATCGATCCGGAA
GCCTACCTTTGCCATATCCTGAGCCTACTGCCGGAATGGCCCAGCAACAAAGTGGCGGAACTGCTGCCATGGAACGTGGTTCTTACCGATAAATAACCGT
CAATACGGCGCTCACTTAACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
441 bp146 aa86526+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MENAANWRTESRTVYSNDFKLRMVELASRPDANVAQLAREHGVDNNLIFKWLRLWQREGRISRRMPATIVGPVVPQPLPVSPTLLSVDVINDPQPIAEND
TLCTFSSTHSSATSCHVEFRHGKMTLENPSSELLAVLIRELTGRTQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
351 bp116 aa523873+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MISLPSGTRIWLVAGATDMRKSFNGLGEQIQHVLDEAPFSGHLFIFRGRRGDTVKILWADADGLCLFIKRLEEGQFVWPTVRDGKIAITRSQLAMLLDKL
DWRQPKTARLNSLTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1593 bp530 aa9042496+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQDYPARIAALEDALRQKDSQLSLIAETESFLRSALSRAEEKIENEEREIEHLRA*IEKLRRMLFGTRSEKLRRQVEEAEALLKLQEQQSDRYNGRDND
QQVPRQLRQSRHRRPLPEHLPREIHRLEPVERCCPDCGSDMKYLSEVSAEQLELVSSALKVIRTVRVKKACTRCDCIVEAPAPSRPIDRGIAGPGLLARV
LTAKYCEHTPLYRQTEILARQGVELSRALLSNWVDACCRLMAPLDDALYHYVMDCRKLHTDDTPVPVLAPGRKKTKTGRIWTYVRDDRNVGSSDPPAAWF
AFSPDRQGKHPQQHLRYYHGVLQADAFAGYDRLFSAERDGGPLTEAACWAHARRKIHDVYISTHTATAEEALKRIGELYAIEEAIRGLPATERLAARQSR
SKPLLISLHDWLVEKSATLSKKSRLGEAFAYALNQWDALCYYCDDGLAEPDNNAAERALRAVCLGKKNFIFFGSDHGGERGALLYGLIGTCRLNGIDPEA
YLCHILSLLPEWPSNKVAELLPWNVVLTDK

 

Blast result :Comments : Internal stop codon due to SNP compared with transposase in ISKox3. 100% identical identical sequence in 11 other sequences in GenBank, suggesting that it is real - possible fusion protein?
Comments
ISKpn33 is 95% aa similar to ISEcl6.
References
1] Sally Partridge (2018) Direct submission.
2] Sandegren,L., Linkevicius,M., Lytsy,B., Melhus,A. and Andersson,D.I. (2012) J. Antimicrob. Chemother. 67 (1), 74-83.