ISPye40
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DENX01000005 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei 32053 Paracoccus sp. UBA2633 |
DNA section
IS Length : 852 bp
Ends
IR Length : 16/18
IRL : GGTCATGTTGACAAAAGGGATTCCGCTGGTCGGCGACTTGTGATTCAAGC
IRR : GGTCATTTTGACAAATGGTGGAGCCAGAGCCGGATGCATGCTATGTCGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ctaaatttcg | TA | gaaacggcgc | 2 |
| gcacggtgct | TA | aaattctaat | 2 |
DNA sequence
GGTCATGTTGACAAAAGGGATTCCGCTGGTCGGCGACTTGTGATTCAAGCTCATCTCTACGTGGGAGATGGAGCTTGGCGCGCAACCTGATATCCGACAG
TGAGTGGGCCTTCTTCGAAAGCTTTATTCGTGCCGTTCGACACCCGCATGGGCGGAAGCCTGTGGATCATCGTCTTGTTCTGGATGGTATTTTCTGGATT
GCAAGAACTGGTGCGCCCTGGCGCGACCTGCCCGAAGAGTTCGGCAAGTGGTCGTCGGTCTACCGTCAGTTCCGCCGCTGGACCCTAGCGGGGCTGTGGG
AGGACATTCTGGACGCGCTGAACCACGCGGGAATTGCACCCGACAAGCTCCAGATGGTCGACAGCACCGTGATCTGCGCCCATCATCATGCGGCGGGCGC
AAAAGGGGGACTCCGAAAGAGGCTCTTGGCCGTTCGCGAGGTGGGTTCTCGACCAAGATCCATCTCCGCGTCAACGGCGCTGGCCTCCCGATGAGGACCG
AGATCACGCCGGGCCAGGATTCCGACTATACCGGCTATGATCTGGTGATGGCCGACAACCTGCCGCAGCCTGCGGTTCTGGTGGCGGACAGGGGCTATGA
CTCTGATAAAATTCGGGAAGATATCGAAAGCCGCAACGCCCTGTCGATGATCCCGATGCGCAAGAACCGCAAGGTTCGAACGGTTGTCAACATGACCATC
TATACGCTGCGCAACATGGTCGAGCGGTGCTTCAACAAACTCAAGAACAGCCGTCGGCTGGCAACCCGCTACGACAAAACCGCAGCCAGCTTCCTCGGCT
TCGTCGACATAGCATGCATCCGGCTCTGGCTCCACCATTTGTCAAAATGACC
TGAGTGGGCCTTCTTCGAAAGCTTTATTCGTGCCGTTCGACACCCGCATGGGCGGAAGCCTGTGGATCATCGTCTTGTTCTGGATGGTATTTTCTGGATT
GCAAGAACTGGTGCGCCCTGGCGCGACCTGCCCGAAGAGTTCGGCAAGTGGTCGTCGGTCTACCGTCAGTTCCGCCGCTGGACCCTAGCGGGGCTGTGGG
AGGACATTCTGGACGCGCTGAACCACGCGGGAATTGCACCCGACAAGCTCCAGATGGTCGACAGCACCGTGATCTGCGCCCATCATCATGCGGCGGGCGC
AAAAGGGGGACTCCGAAAGAGGCTCTTGGCCGTTCGCGAGGTGGGTTCTCGACCAAGATCCATCTCCGCGTCAACGGCGCTGGCCTCCCGATGAGGACCG
AGATCACGCCGGGCCAGGATTCCGACTATACCGGCTATGATCTGGTGATGGCCGACAACCTGCCGCAGCCTGCGGTTCTGGTGGCGGACAGGGGCTATGA
CTCTGATAAAATTCGGGAAGATATCGAAAGCCGCAACGCCCTGTCGATGATCCCGATGCGCAAGAACCGCAAGGTTCGAACGGTTGTCAACATGACCATC
TATACGCTGCGCAACATGGTCGAGCGGTGCTTCAACAAACTCAAGAACAGCCGTCGGCTGGCAACCCGCTACGACAAAACCGCAGCCAGCTTCCTCGGCT
TCGTCGACATAGCATGCATCCGGCTCTGGCTCCACCATTTGTCAAAATGACC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 420 bp | 139 aa | 75 | 494 | + | No |
Description : First part of the transposase
ORF sequence :
LARNLISDSEWAFFESFIRAVRHPHGRKPVDHRLVLDGIFWIARTGAPWRDLPEEFGKWSSVYRQFRRWTLAGLWEDILDALNHAGIAPDKLQMVDSTVI
CAHHHAAGAKGGLRKRLLAVREVGSRPRSISASTALASR
CAHHHAAGAKGGLRKRLLAVREVGSRPRSISASTALASR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 747 bp | 248 aa | 104 | 850 | + | No |
Description : Second part of the transposase
ORF sequence :
VGLLRKLYSCRSTPAWAEACGSSSCSGWYFLDCKNWCALARPARRVRQVVVGLPSVPPLDPSGAVGGHSGRAEPRGNCTRQAPDGRQHRDLRPSSCGGRK
RGTPKEALGRSRGGFSTKIHLRVNGAGLPMRTEITPGQDSDYTGYDLVMADNLPQPAVLVADRGYDSDKIREDIESRNALSMIPMRKNRKVRTVVNMTIY
TLRNMVERCFNKLKNSRRLATRYDKTAASFLGFVDIACIRLWLHHLSK
RGTPKEALGRSRGGFSTKIHLRVNGAGLPMRTEITPGQDSDYTGYDLVMADNLPQPAVLVADRGYDSDKIREDIESRNALSMIPMRKNRKVRTVVNMTIY
TLRNMVERCFNKLKNSRRLATRYDKTAASFLGFVDIACIRLWLHHLSK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 776 bp | 258 aa | 75 | 850 | + | Yes |
Chemistry : DDE
ORF sequence :
LARNLISDSEWAFFESFIRAVRHPHGRKPVDHRLVLDGIFWIARTGAPWRDLPEEFGKWSSVYRQFRRWTLAGLWEDILDALNHAGIAPDKLQMVDSTVI
CAHHHAAGAKRGTPKEALGRSRGGFSTKIHLRVNGAGLPMRTEITPGQDSDYTGYDLVMADNLPQPAVLVADRGYDSDKIREDIESRNALSMIPMRKNRK
VRTVVNMTIYTLRNMVERCFNKLKNSRRLATRYDKTAASFLGFVDIACIRLWLHHLSK
CAHHHAAGAKRGTPKEALGRSRGGFSTKIHLRVNGAGLPMRTEITPGQDSDYTGYDLVMADNLPQPAVLVADRGYDSDKIREDIESRNALSMIPMRKNRK
VRTVVNMTIYTLRNMVERCFNKLKNSRRLATRYDKTAASFLGFVDIACIRLWLHHLSK
Blast result :
Comments
ISPye40 is 96% aa similar to ISPbe2.
ISPye40 was identified in Paracoccus sp. UBA2633 (whole genome sequencing) and Paracoccus yeei strain 32053 (isoform). The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPye40 was identified in Paracoccus sp. UBA2633 (whole genome sequencing) and Paracoccus yeei strain 32053 (isoform). The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References