ISPye44

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Paracoccus yeei
Paracoccus yeei
Paracoccus yeei CCUG 32053
Paracoccus yeei CCUG 17731
DNA section
IS Length : 3167 bp

Ends


IR Length : 14/21

IRL : GTAAGCTCCCGGTGAGGACCGTCTGACGCTGAAGGCGGGATGGATGTAGG
IRR : GTAACCATCCGGCGTAGGCCGCGGACGGGTGTCCGTGCTCAGGGAGTGGG

Insertion site


Left flankDirect repeatRight flankDR Length
gaacctgagctcgcaaacgt0
caccctctccggtgtgtcca0
cggatctcgggcaggatctg0
caccctctccttttttgtta0
caccctctccggtgtgtcca0

DNA sequence

GTAAGCTCCCGGTGAGGACCGTCTGACGCTGAAGGCGGGATGGATGTAGGAGGCTGAAGCTATGGACACCCATAGCTTCAGTTCCCAAGTCGAGGTTCTC
TCGGTGACCGATAGTGGTCGGCGGCGTCGCTGGTCGGCTGCCGAGAAGATCCGTATCGTCGAGGAAAGCCTGTCGCGCCCGCGCAGCGTGGCGCTGACGG
CGCGGCGTCACGATATCTCTCGCGCGTTGCTGACGCGCTGGCGGAAGGACTATCGCGAGGGCCTTCTCGGAAATGAGCCTGCCCTGACCTTTGCGCCGGT
GATGATTGCTGCCGAACCTGTGCCTGTATCGGTATCACCTGCGGCAGAGCTGGAGATCTCCGATGCCGTCACAGTCGCGATCACACTGGTGAATGGCCGG
CACCTCGTGGTTCCTGCGGGAATTGACCCGGTCATTCTGGCGCGGCTGCTTCCGGTGCTGGACAGCGCATGATCGCCTTTCCGGCGGGGGCGCGCGTGTG
GATTGCAGGCGGGGTGACGGACATGCGGTGCGGGATGAACAGCCTGGCGCTGAAGGTCCAGCAGGGCCTGGGTCGCGATCCGCACGGTGGCGAGATCTTC
TGCTTCCGGGGGCGCAAGGGTGACCTGATCAAGGTGTTGTGGCACGACGGGGTCCGCATGTCGCTGTATCTGAAGCGGCTGGAGGCGGGGAAGTTCATCT
GGCCGGTGAGCCAGAATGGCTCGTCCGTTCCGGTTTCGGCGGCGCAGCTCGGCTATCTTCTGGAAGGAATCGACTGGCGCAATCCGCGCTGGACGCAGCG
GCCTGCCTCGGCGGGTTGATAGCCAAAATACCCTTGTTATATTGGACTATTCCGGGATTCCGTGATAGGTCTTCGGCATGTCGGAGACCGCATCGGAGAT
CGCTGTTCTGCGCGCCGCACTCGCCGCGGCAGAGGCTCGCGCATCTGCCGTAGAGGGCGCTTTGGTCGCCGCTAAGGACGAACTGACCCAGGTTCAGGCG
ATCGTCTCGGCCTCCGAAGACATGATCCGGCACCTCCGACTGCAGATCGCCAAACTCCGCCGCGAGCAATATGGCCATGGTGCCGAACGCCATGCCCGGC
TGATCGAGCAACTGGAGATGCAGCTCGAAGACATCGAGACCGACATTGCCGATGACCGGGCCAAAGCCGAGGCGACGGGCCCGAGGGCGATGGTCGCGGC
CTTCGAACGCCGCCGCCCAGCCCGCAAGCCGTTCCCCGGGCATTTGCCCCGCGAGCGCGTCGTGGTTAAGGCGCCGACTTCCTGCACTTGCTGTGGTTCG
GCGCGCATCGTGAAGATGGGCGAGGACATCACCGAGACGCTGGAGGTGATCCCGCGGCAGTGGAAGGTCGTGCAAACCGTCCGCGAGAAGTTCACCTGCC
GCGACTGCGAGAAGATCAGCCAGTCGCCGGCGCCGTTTCATCCGACCCCAAGGGGCCGGGCCGGGCCGAACCTGCTGGCCATGATCCTGTTCGAGAAGTT
CGGCCAGCATCAGCCCCTGAACAGGCAGGCCGAGCGCTATGCGAAGGAGGGCGTCGAGATCAGCCTCTCGACCCTGGCCGATCAGGTCGGGGCCTGTGCC
GTGGCGCTGCAGCCGATCCACGATCTGATCCGTGCCCATGTCCTCGGCGCCGAGCGATTGCATGCTGACGACACGACCGTGCCGCTGCTGGCCAAAGGTG
GCACGCAAACCGCCCGGCTCTGGACCTATCTGCGGGATGACCGGCCCTTTGCCGGTGGGGCGCCGCCGGCAGCGCTCTATTACTTCTCAACCGACCGCAG
GAAAGAACACCCGACCCGTCATCTGGCGGGCTGGACCGGCACCCTGCAAGCCGATGCCTATGGCGGTTACAACGACCTCTACCATGCCAGCCACAGGCCC
GCGCCGGTGCTGAGCGCGCTGTGCTGGTCGCATGCCCGGCGCAAGTTCTTCGAACTGGCGGATATCAAAGCCACGGCCCGCAAGGGCAGATCGGTGGCCG
AGGAGATCTCGCCCATCGCGCTGGAAGCGGTCAAACGCTTCGACGCCATCTTCGATGCCGAGCGCGAGATCACCGGCCTGACCGCCGAGGCCCGCCATGA
CGCCCGTCAGCGGCTGGTGCGCCCGATGGTCCAGGATCTGCACAACTGGTTGCGGACCGAACGCGCCCGAATGTCGAAGCACAACCCCGTCGCCAAGGCG
ATCAACTACATGTTCGAGGAGGACGGCCGCTGGGACGCCTTCACCTGCTTCCTCGATGATGGCCGCATCTGCCTTACGAACAACGCAGCAGAACGCGCGC
TGCGGGGCGTCGCCCTCGGGCGGAAAGCCTGGCTCTTCGCCGGATCTCCGCGCGGTGGCGACCGCGCCGCCTTCATGTACTCCCTGATCGTCACGGCCAA
GATGAATGACGTCGACCCGCAGGCTTGGCTGGCCGATGTCCTTGCCCGGCTGCCGGACATACCCCTGTCGCGCCTGCCGGAACTTCTTCCCTGGAACTGG
TCGCAAATACCGCAAAGGAGGGCCGCATGACCCTGATCGCCCGCCTCCGGATCCTTCTGAACGACGTCGATCCTCAGCCGATGCGCCATATCGAGGTGCC
GCTGAAGATCAGGCTCGACCGGCTGCATGAGGTGGTCCAGGCGGCGATGGGCTGGACGGACACCCATCTCTACGAATTCAGGGCCGGGGGGGCGGGCTGG
GGCGTGCCCGATCCGGACGGATTCTACGACGGCCCGATGCCCGCGAAGAAGGCGACGCTGCAAAAGGTGCTCGAGGATACCGGCGCCAGGACGCTCCAGT
ATGTCTACGACTTCGGCGATGACTGGGACCACAGCATCCGGATCGAGCGGGTCACCGAGGCCACCCCGGGCGTGACCTATCCAAGGCTTCTGAAGGCCAG
CGGGGCCTGCCCGCCCGAGGACGTCGGCGGCGCCCCGGGCTACGAAGCGTTCCTTGAAGCCCTCGCAGATCCGGACCACGAACAGCACGACGACATGGTC
GGCTGGTCAGGTGCATCCTTCGATCCCGAAGATGCCAGGATCAACAGGATCGTCGAACGCTTTGACCAACTCGCGAAGAAATGGTCCCCGCGGCCCGGCA
AGCCCAAAGCACCCAAGCCCACTCCCTGAGCACGGACACCCGTCCGCGGCCTACGCCGGATGGTTAC
Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
411 bp136 aa62472+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MDTHSFSSQVEVLSVTDSGRRRRWSAAEKIRIVEESLSRPRSVALTARRHDISRALLTRWRKDYREGLLGNEPALTFAPVMIAAEPVPVSVSPAAELEIS
DAVTVAITLVNGRHLVVPAGIDPVILARLLPVLDSA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
350 bp116 aa469818+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIAFPAGARVWIAGGVTDMRCGMNSLALKVQQGLGRDPHGGEIFCFRGRKGDLIKVLWHDGVRMSLYLKRLEAGKFIWPVSQNGSSVPVSAAQLGYLLEG
IDWRNPRWTQRPASAG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1653 bp550 aa8782530+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSETASEIAVLRAALAAAEARASAVEGALVAAKDELTQVQAIVSASEDMIRHLRLQIAKLRREQYGHGAERHARLIEQLEMQLEDIETDIADDRAKAEAT
GPRAMVAAFERRRPARKPFPGHLPRERVVVKAPTSCTCCGSARIVKMGEDITETLEVIPRQWKVVQTVREKFTCRDCEKISQSPAPFHPTPRGRAGPNLL
AMILFEKFGQHQPLNRQAERYAKEGVEISLSTLADQVGACAVALQPIHDLIRAHVLGAERLHADDTTVPLLAKGGTQTARLWTYLRDDRPFAGGAPPAAL
YYFSTDRRKEHPTRHLAGWTGTLQADAYGGYNDLYHASHRPAPVLSALCWSHARRKFFELADIKATARKGRSVAEEISPIALEAVKRFDAIFDAEREITG
LTAEARHDARQRLVRPMVQDLHNWLRTERARMSKHNPVAKAINYMFEEDGRWDAFTCFLDDGRICLTNNAAERALRGVALGRKAWLFAGSPRGGDRAAFM
YSLIVTAKMNDVDPQAWLADVLARLPDIPLSRLPELLPWNWSQIPQRRAA

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
603 bp200 aa25273129+No
ORF function : Passenger Gene
Annotation : Plasmid pRiA4b ORF-3-like proteinDescription :

ORF sequence :

MTLIARLRILLNDVDPQPMRHIEVPLKIRLDRLHEVVQAAMGWTDTHLYEFRAGGAGWGVPDPDGFYDGPMPAKKATLQKVLEDTGARTLQYVYDFGDDW
DHSIRIERVTEATPGVTYPRLLKASGACPPEDVGGAPGYEAFLEALADPDHEQHDDMVGWSGASFDPEDARINRIVERFDQLAKKWSPRPGKPKAPKPTP

 

Blast result :
Comments
ISPye44 is 86% (transposase) aa similar to ISRel24.
ISPye44 was identified by in silico sequence analysis of Paracoccus yeei strains CCUG 32053 (4 copies and isoforms) and 17731 (3 copies).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.