ISMin1
- Family IS1380
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002275 | ND | Mycobacterium indicus | Mycobacterium intracellulare Mycobacterium indicus pranii MTCC 9506 |
DNA section
IS Length : 1614 bp
Ends
IR Length : 24/28
IRL : CCTCGATCTTTCGTGAAGATGGGGTGGTGACGGGCGTGTGATGCACGGAA
IRR : CCTCGATCTTTCTTGAGGTGGGTTGGTTGTTGTCGATTGGTCGGTTGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCTCGACGC | CTAA | CGCGCGAAAC | 4 |
DNA sequence
CCTCGATCTTTCGTGAAGATGGGGTGGTGACGGGCGTGTGATGCACGGAAGTGCCTGTCCTGCTTGAGAAAATGTGACTTGTCTAGGGTCCATGTTCTGC
AAGCGGGAAGGCACTCCGTAGGTGAAGCGTAGCCGTTGTGGTGGGTTTAGGGTCGAGGCTGGTCGCGAGTCGTTGATTTCCTCGACTGGTGCGGCGCTGC
TCGTGCAGACTGCGCAGGTCAGCGGCTTGACGTCGGTTCTCTCGGATCAGCTGCGGTCGTGGCGGGCGCCGCGCTCGGTCCACGATCCGGGCAAAATGGT
GCTGGATCTGGCGGTGGCGATCGCGCTCGGCGGTGACTGCTTGGCCGATGCGGCGCTGCTGCGTGCTCAGCCGGAGTTGTTCGGTGCGGTGGCCTCTGAT
CCCACGATCAGCCGGCTGCTGGAGACTCTCGGCGGGGATCCCGCCGCGGCGATCGCCGCGATCCGTCATGCGCGGGCGAGCGCGCGGGCGACGGTGTGGC
AGCTGTGCTGCCCGGTTCCTGTAGCTGGGGAGGTCGTCGTCGATCTGGACGCCACCTTGATCGGGGCGCATTCGGATAAAGAAGGCGCGACCCCGAACTT
CAAGCGGGGATACGGGTTTCATCCGATGATGGCGTTCGTTGATCACGGTGCCGGCGGTACCGGGGAGCCGTTGGCGGCGATGTTGCGTCCCGGGCGGGCC
AACGCCAGCGACGCCGCCGATCAGATCGCGGTCCTTGATGCCGCGTTGGCCCAACTGCCCCAGGGGGTGCGCGATCGGGTGCTGGTGCGAGGCGACACCG
GTTCGGGAGTCAAGGAATTCCTCTGGCACATCCACCACCTGGGTCTGTCCTACTCGGTCGGTGTCTACGGTCGCCAACCCGTGCTGGATGCCCTGGCCGC
CCTGCCGCGCCAGGCCTGGCGGCGCGCTAAGGACGCCGATGGGTGTCTGCGCGAGGGCGCTCAGGTTGCCGAACTGACCCGCTGGCTGCCGGCGACGTTC
GTTGGCTGGCCGCCAGGGATGCGGGTGATCGCCCGCCGGGAACGCCCACATCCCGGTGCCCAACTGCGCATCACCGATGCCCACGGCTGGCGCATCACGA
TGTTCGCCACCAACACCACCGGCGGGCGACTCGCCGACCTCGAAGTACGCCACCGACTGCGGGCCCGCGCCGAGGACCGCATCCGCACCCTCAAAGACAC
CGGCCTGGCCAACCTGCCGCTGCAGGCCTTCGGCAAGAACGAGATCTGGCTCGAGCTGGCCGCTCTGGCCTACGAGCTGCTGACCTGGACCCAACTACTG
GCCTGGCCCGATCACCCCGCCCGCAGCTGGGAACCCAAACGACTGCGCCTGCGCCTGCTTTCGGTGGCCGGCCGGGTCATCACCACCGGACGCCGTCGCA
TCCTGCGCCTGAGCAGACGATGGCCCTGGTCAGAACTACTGACCAGGGGTCAACGAAACCTCGCCGCACTCAATTGAATTCACCGCAACATCCCTACGAC
CCAGGACCCAGGAGAACCGGCGACCCAGCGCCGGGGACGCTCCACGCCCAACCCACCGCACCGGCAACAACCGACCAATCGACAACAACCAACCCACCTC
AAGAAAGATCGAGG
AAGCGGGAAGGCACTCCGTAGGTGAAGCGTAGCCGTTGTGGTGGGTTTAGGGTCGAGGCTGGTCGCGAGTCGTTGATTTCCTCGACTGGTGCGGCGCTGC
TCGTGCAGACTGCGCAGGTCAGCGGCTTGACGTCGGTTCTCTCGGATCAGCTGCGGTCGTGGCGGGCGCCGCGCTCGGTCCACGATCCGGGCAAAATGGT
GCTGGATCTGGCGGTGGCGATCGCGCTCGGCGGTGACTGCTTGGCCGATGCGGCGCTGCTGCGTGCTCAGCCGGAGTTGTTCGGTGCGGTGGCCTCTGAT
CCCACGATCAGCCGGCTGCTGGAGACTCTCGGCGGGGATCCCGCCGCGGCGATCGCCGCGATCCGTCATGCGCGGGCGAGCGCGCGGGCGACGGTGTGGC
AGCTGTGCTGCCCGGTTCCTGTAGCTGGGGAGGTCGTCGTCGATCTGGACGCCACCTTGATCGGGGCGCATTCGGATAAAGAAGGCGCGACCCCGAACTT
CAAGCGGGGATACGGGTTTCATCCGATGATGGCGTTCGTTGATCACGGTGCCGGCGGTACCGGGGAGCCGTTGGCGGCGATGTTGCGTCCCGGGCGGGCC
AACGCCAGCGACGCCGCCGATCAGATCGCGGTCCTTGATGCCGCGTTGGCCCAACTGCCCCAGGGGGTGCGCGATCGGGTGCTGGTGCGAGGCGACACCG
GTTCGGGAGTCAAGGAATTCCTCTGGCACATCCACCACCTGGGTCTGTCCTACTCGGTCGGTGTCTACGGTCGCCAACCCGTGCTGGATGCCCTGGCCGC
CCTGCCGCGCCAGGCCTGGCGGCGCGCTAAGGACGCCGATGGGTGTCTGCGCGAGGGCGCTCAGGTTGCCGAACTGACCCGCTGGCTGCCGGCGACGTTC
GTTGGCTGGCCGCCAGGGATGCGGGTGATCGCCCGCCGGGAACGCCCACATCCCGGTGCCCAACTGCGCATCACCGATGCCCACGGCTGGCGCATCACGA
TGTTCGCCACCAACACCACCGGCGGGCGACTCGCCGACCTCGAAGTACGCCACCGACTGCGGGCCCGCGCCGAGGACCGCATCCGCACCCTCAAAGACAC
CGGCCTGGCCAACCTGCCGCTGCAGGCCTTCGGCAAGAACGAGATCTGGCTCGAGCTGGCCGCTCTGGCCTACGAGCTGCTGACCTGGACCCAACTACTG
GCCTGGCCCGATCACCCCGCCCGCAGCTGGGAACCCAAACGACTGCGCCTGCGCCTGCTTTCGGTGGCCGGCCGGGTCATCACCACCGGACGCCGTCGCA
TCCTGCGCCTGAGCAGACGATGGCCCTGGTCAGAACTACTGACCAGGGGTCAACGAAACCTCGCCGCACTCAATTGAATTCACCGCAACATCCCTACGAC
CCAGGACCCAGGAGAACCGGCGACCCAGCGCCGGGGACGCTCCACGCCCAACCCACCGCACCGGCAACAACCGACCAATCGACAACAACCAACCCACCTC
AAGAAAGATCGAGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1305 bp | 434 aa | 173 | 1477 | + | No |
Chemistry : DDE
ORF sequence :
MISSTGAALLVQTAQVSGLTSVLSDQLRSWRAPRSVHDPGKMVLDLAVAIALGGDCLADAALLRAQPELFGAVASDPTISRLLETLGGDPAAAIAAIRHA
RASARATVWQLCCPVPVAGEVVVDLDATLIGAHSDKEGATPNFKRGYGFHPMMAFVDHGAGGTGEPLAAMLRPGRANASDAADQIAVLDAALAQLPQGVR
DRVLVRGDTGSGVKEFLWHIHHLGLSYSVGVYGRQPVLDALAALPRQAWRRAKDADGCLREGAQVAELTRWLPATFVGWPPGMRVIARRERPHPGAQLRI
TDAHGWRITMFATNTTGGRLADLEVRHRLRARAEDRIRTLKDTGLANLPLQAFGKNEIWLELAALAYELLTWTQLLAWPDHPARSWEPKRLRLRLLSVAG
RVITTGRRRILRLSRRWPWSELLTRGQRNLAALN
RASARATVWQLCCPVPVAGEVVVDLDATLIGAHSDKEGATPNFKRGYGFHPMMAFVDHGAGGTGEPLAAMLRPGRANASDAADQIAVLDAALAQLPQGVR
DRVLVRGDTGSGVKEFLWHIHHLGLSYSVGVYGRQPVLDALAALPRQAWRRAKDADGCLREGAQVAELTRWLPATFVGWPPGMRVIARRERPHPGAQLRI
TDAHGWRITMFATNTTGGRLADLEVRHRLRARAEDRIRTLKDTGLANLPLQAFGKNEIWLELAALAYELLTWTQLLAWPDHPARSWEPKRLRLRLLSVAG
RVITTGRRRILRLSRRWPWSELLTRGQRNLAALN
Blast result :
Comments
ISMin1 is 77% aa similar to ISMgi1.
The sequence was first described in Mycobacterium intracellulare (Accession number : AB519649), but the transposase carried unexpected frameshift.
The sequence was first described in Mycobacterium intracellulare (Accession number : AB519649), but the transposase carried unexpected frameshift.
References
1] ISfinder submission (2018)
2] Ahmed,N., Saini,V., Raghuvanshi,S., Khurana,J.P., Tyagi,A.K., Tyagi,A.K. and Hasnain,S.E. (2007) PLoS ONE 2 (10), E968
3] Saini,V., Raghuvanshi,S., Khurana,J.P., Ahmed,N., Hasnain,S.E.,Tyagi,A.K. and Tyagi,A.K. (2012) Nucleic Acids Res. 40 (21), 10832-10850.
4] Ichikawa,K., Yagi,T., Inagaki,T., Moriyama,M., Nakagawa,T., Uchiya,K., Nikai,T. and Ogawa,K. (2010) Microbiology (Reading, Engl.) 156 (PT 2), 496-504.
2] Ahmed,N., Saini,V., Raghuvanshi,S., Khurana,J.P., Tyagi,A.K., Tyagi,A.K. and Hasnain,S.E. (2007) PLoS ONE 2 (10), E968
3] Saini,V., Raghuvanshi,S., Khurana,J.P., Ahmed,N., Hasnain,S.E.,Tyagi,A.K. and Tyagi,A.K. (2012) Nucleic Acids Res. 40 (21), 10832-10850.
4] Ichikawa,K., Yagi,T., Inagaki,T., Moriyama,M., Nakagawa,T., Uchiya,K., Nikai,T. and Ogawa,K. (2010) Microbiology (Reading, Engl.) 156 (PT 2), 496-504.