ISMba1
- Family IS4
- Group ISH8
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP009526 | ND | Methanosarcina barkeri | Methanosarcina barkeri str. Wiesmoor |
DNA section
IS Length : 1539 bp
Ends
IR Length : 10/11
IRL : CTGTTCTCAAATTTAACAAAAACCTTTTCAAAAACCTTTTAAATATGTAA
IRR : CTGTTCTCTAACTGTATTTTTGACAGGGACAGTCATAGCTTTCTTTATTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATAAAAAGA | TATTTTTTTA | 0 |
DNA sequence
CTGTTCTCAAATTTAACAAAAACCTTTTCAAAAACCTTTTAAATATGTAAGGCAAGAAGAAGCCCAGAGGTGCTGTATCTACTGCATCCCCCAATACAGT
CTCTCCGAATTTATGCTCCCCCAAGCACGTTGATTCGAAGAATAATCAGCAGCCTCCAGGAAATGTATATGCTTTTATAATAATATTCTAATCTTTGTAT
CTATGTCTCCTCGTTCTCCACCTACTCTTGAGGACTCTCTTCGGGAAATGTTTCCCGAAGAGTGGTTAAGGCAAACTGCCAGAGAAACTGGTCTTATAAT
ACGTGAACGTAAAATTGACCCTGTCATCATCTTTTGGGTTTTAACTCTGAGTTTTGGTGTACGCTTGCAGCGTACACTTGCCAGTTTGAAACGAGAATAT
GAAACTGAGTCTCATAAAATCATAAGCGATAGCAGTTGGTACTATCGTTTCACTCCAGAACTTGTTGAGTTCCTACATCAGTGTGTAATTCACGGCATAG
AAGAACTTGCAAAAGAACCTGGTAGAAAACTTAGCAAGAAACTCGAAAACTTCCAAGATGTTCTCATTCAGGACAGTACAATTGTTCGTCTTCACTCTTC
TTTAGCAGACAAATTTCCAGTAGCAAGAACAAGAAAAGTAGCTGCAGGCATAAAAGTGGGGGTTATGGTAAGCGCAGTTGCTAACGGACCTAAAACAGTT
GCCCTGTATTCTGAAAAAACAGCTGAGATCAAGACATTAAAAATAGGTCCCTGGATCAAAGATCGTATTCTGCTTGTTGATCTTGGTTTTTACAAAACTC
AGATGTTTGCAAGGGTTGAGGAAAATGGAGGATATTTTGTTTCAAGAATAAGGAAAAATATGGATCCTATTCTTGTTTCTGTTGAAGAGGGACTTTCTAA
GACAAAAAGCAAGGAGTTCGCTGGAAAAACTGTTAGTGAATGCATTAAACAACTTTCTGGGAAAGATCTTGATGCTGTTGTGAAAATAAAGTTTAAAAGA
AGAGTATACAAAGGTAAACAAAAACAGGATGAGATGATTGTACGTCTTGTTGCAGTCTATAATGACGAGGATGAAAAGTACCATATTTATATCACAAATA
TTCAGAAAGATATTTTGAATGCAAAAGATATTGCAAACCTATATGGAGCAAGATGGGACATAGAACTGCTTTTCAAAGAATTGAAAAGCAAATACGCGCT
AGACGTTCTTGAAACAAAGAATGTGCAGGTAATTGAAGCTCTAATCTGGACAGCAATATTGGCACTAATCGTTAGCAGAAGAATATATTCTCTTGTAAGA
AACTCAACAACTCATCCTGAAAAAATGGCTAGATATACACAGTTACGTTGGAGTACAATATTTGCAGAGAATGTATCAGATTTGTTGACAGTAATTCTGC
ATATATGTGGGATTCAGAGGACTTTTGAAACGATATATGGTGCTTTTATTCTTAACTCTCAGTCAAGCGTTTTAATCCCTTTTTATCATTAATAAAGAAA
GCTATGACTGTCCCTGTCAAAAATACAGTTAGAGAACAG
CTCTCCGAATTTATGCTCCCCCAAGCACGTTGATTCGAAGAATAATCAGCAGCCTCCAGGAAATGTATATGCTTTTATAATAATATTCTAATCTTTGTAT
CTATGTCTCCTCGTTCTCCACCTACTCTTGAGGACTCTCTTCGGGAAATGTTTCCCGAAGAGTGGTTAAGGCAAACTGCCAGAGAAACTGGTCTTATAAT
ACGTGAACGTAAAATTGACCCTGTCATCATCTTTTGGGTTTTAACTCTGAGTTTTGGTGTACGCTTGCAGCGTACACTTGCCAGTTTGAAACGAGAATAT
GAAACTGAGTCTCATAAAATCATAAGCGATAGCAGTTGGTACTATCGTTTCACTCCAGAACTTGTTGAGTTCCTACATCAGTGTGTAATTCACGGCATAG
AAGAACTTGCAAAAGAACCTGGTAGAAAACTTAGCAAGAAACTCGAAAACTTCCAAGATGTTCTCATTCAGGACAGTACAATTGTTCGTCTTCACTCTTC
TTTAGCAGACAAATTTCCAGTAGCAAGAACAAGAAAAGTAGCTGCAGGCATAAAAGTGGGGGTTATGGTAAGCGCAGTTGCTAACGGACCTAAAACAGTT
GCCCTGTATTCTGAAAAAACAGCTGAGATCAAGACATTAAAAATAGGTCCCTGGATCAAAGATCGTATTCTGCTTGTTGATCTTGGTTTTTACAAAACTC
AGATGTTTGCAAGGGTTGAGGAAAATGGAGGATATTTTGTTTCAAGAATAAGGAAAAATATGGATCCTATTCTTGTTTCTGTTGAAGAGGGACTTTCTAA
GACAAAAAGCAAGGAGTTCGCTGGAAAAACTGTTAGTGAATGCATTAAACAACTTTCTGGGAAAGATCTTGATGCTGTTGTGAAAATAAAGTTTAAAAGA
AGAGTATACAAAGGTAAACAAAAACAGGATGAGATGATTGTACGTCTTGTTGCAGTCTATAATGACGAGGATGAAAAGTACCATATTTATATCACAAATA
TTCAGAAAGATATTTTGAATGCAAAAGATATTGCAAACCTATATGGAGCAAGATGGGACATAGAACTGCTTTTCAAAGAATTGAAAAGCAAATACGCGCT
AGACGTTCTTGAAACAAAGAATGTGCAGGTAATTGAAGCTCTAATCTGGACAGCAATATTGGCACTAATCGTTAGCAGAAGAATATATTCTCTTGTAAGA
AACTCAACAACTCATCCTGAAAAAATGGCTAGATATACACAGTTACGTTGGAGTACAATATTTGCAGAGAATGTATCAGATTTGTTGACAGTAATTCTGC
ATATATGTGGGATTCAGAGGACTTTTGAAACGATATATGGTGCTTTTATTCTTAACTCTCAGTCAAGCGTTTTAATCCCTTTTTATCATTAATAAAGAAA
GCTATGACTGTCCCTGTCAAAAATACAGTTAGAGAACAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1290 bp | 429 aa | 203 | 1492 | + | No |
Chemistry : DDE
ORF sequence :
MSPRSPPTLEDSLREMFPEEWLRQTARETGLIIRERKIDPVIIFWVLTLSFGVRLQRTLASLKREYETESHKIISDSSWYYRFTPELVEFLHQCVIHGIE
ELAKEPGRKLSKKLENFQDVLIQDSTIVRLHSSLADKFPVARTRKVAAGIKVGVMVSAVANGPKTVALYSEKTAEIKTLKIGPWIKDRILLVDLGFYKTQ
MFARVEENGGYFVSRIRKNMDPILVSVEEGLSKTKSKEFAGKTVSECIKQLSGKDLDAVVKIKFKRRVYKGKQKQDEMIVRLVAVYNDEDEKYHIYITNI
QKDILNAKDIANLYGARWDIELLFKELKSKYALDVLETKNVQVIEALIWTAILALIVSRRIYSLVRNSTTHPEKMARYTQLRWSTIFAENVSDLLTVILH
ICGIQRTFETIYGAFILNSQSSVLIPFYH
ELAKEPGRKLSKKLENFQDVLIQDSTIVRLHSSLADKFPVARTRKVAAGIKVGVMVSAVANGPKTVALYSEKTAEIKTLKIGPWIKDRILLVDLGFYKTQ
MFARVEENGGYFVSRIRKNMDPILVSVEEGLSKTKSKEFAGKTVSECIKQLSGKDLDAVVKIKFKRRVYKGKQKQDEMIVRLVAVYNDEDEKYHIYITNI
QKDILNAKDIANLYGARWDIELLFKELKSKYALDVLETKNVQVIEALIWTAILALIVSRRIYSLVRNSTTHPEKMARYTQLRWSTIFAENVSDLLTVILH
ICGIQRTFETIYGAFILNSQSSVLIPFYH
Blast result :
Comments
ISMba1 is 95% aa similar to ISMba6.
References
1] ISfinder submission (2018)
2] Henriksen,J.R., Luke,J., Reinhart,S., Benedict,M.N., Youngblut,N.D., Metcalf,M.E., Whitaker,R.J. and Metcalf,W.W. (2014) Direct GenBank submission.
2] Henriksen,J.R., Luke,J., Reinhart,S., Benedict,M.N., Youngblut,N.D., Metcalf,M.E., Whitaker,R.J. and Metcalf,W.W. (2014) Direct GenBank submission.