ISfinder Mirror Site

ISLbp6

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISLbp7

Accession number	Transposition	Origin	Host
CP015814	ND	Leptospira borgpetersenii	Leptospira borgpetersenii 4E

DNA section

IS Length : 1077 bp

Ends

IR Length : 25/28

IRL : CTCATCCCTATAAAAAAAGTACCCAAAAGAATAGAAAGACTAAAGATATG
IRR : ATCATCCCTATAAAAATAAGTACCTAAATACCGGCTTGTCTCCAACAGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GATCTTGTAT	TA	AAAGATATTA	2

DNA sequence

CTCATCCCTATAAAAAAAGTACCCAAAAGAATAGAAAGACTAAAGATATGCAAAATGAAGAATATAGAAAGAACGAAATTAAAGGGATTAGAAAAACGTC
GATTGAAAGGAATCCAACTTTTAAAGCAAAGTCATACTTGTTATAGAGTAGGAAAAGAACTTTGGGTCAGTAAACAATCGGTAATGCGTTGGCGTGAAAG
ATACGAAGAGGACGGAATAGAAGGGGTAAAGTGGAATGGTCAAAGGGGTCGACCGTCAAAATTAACAAAGTCGCAGAAGAAGGCATTAAAACGCATCATC
TTGAAAGGAGCGACAAGTTATGGATACCCGAATGAACTATGGACAACATTTCGTATAACGGAGATAATTCGAAAAGAATTCAAAGTGAAGTTTCATCAAG
ATTACGTAGGGATTCTGTTACACCAATTAGGATTTTCGTATCAAAAACCAAAGAGAATCGCGTTGGAAAGAGATGAAAAGGCGATTCAAAGTTGGAAAAG
GGAAAGGTGGCCGGATATAAAAAAAAGCGCAGAAAGAGGGATTTGAGATCATATTTCTGGATGAAAGTGGAATCAGTCAAAATCCGTATCCTGCAAAAAC
ATGGGGATTGATAGGTAAGACACCGATCATTCGCCACAAGATGTCTTGGAAAAAATTATCGGTAATAGGGGCTATATCCCAAAAGGATTTTTACTTTCAG
GTTATAGAAGGCTCCGCTAAAAGTCAGAATCTCATTCATTTTTTAAAGATGCTTTTAAAGGAAAATCGTAAAAAGATTTTATTAGTTTGGGATAATCTAT
CTGCCGATAAAAGTAAAACCGTAAATGTATTTTTACAAGAACATACAAAGCGGCTTCGAGTTGAATTTTTACCTCCTTATGCGCCTGAGTTAAATCCCCA
AGAATATATCTGGCGTCGTTGGAAAAGAAACGACATGGCGAATTTTTGTCCGGTTAATTTGAGTTCCTTGTTCAATAGAACCAAATACACTTTAAGAAAA
TTGAAATCTACCACGTTTTGTTTTGAAAGCTGTTGGAGACAAGCCGGTATTTAGGTACTTATTTTTATAGGGATGAT

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
492 bp	163 aa	55	546	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKNIERTKLKGLEKRRLKGIQLLKQSHTCYRVGKELWVSKQSVMRWRERYEEDGIEGVKWNGQRGRPSKLTKSQKKALKRIILKGATSYGYPNELWTTFR
ITEIIRKEFKVKFHQDYVGILLHQLGFSYQKPKRIALERDEKAIQSWKRERWPDIKKSAERGI

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
534 bp	177 aa	521	1054	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKAQKEGFEIIFLDESGISQNPYPAKTWGLIGKTPIIRHKMSWKKLSVIGAISQKDFYFQVIEGSAKSQNLIHFLKMLLKENRKKILLVWDNLSADKSKT
VNVFLQEHTKRLRVEFLPPYAPELNPQEYIWRRWKRNDMANFCPVNLSSLFNRTKYTLRKLKSTTFCFESCWRQAGI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1000 bp	332 aa	55	1054	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKNIERTKLKGLEKRRLKGIQLLKQSHTCYRVGKELWVSKQSVMRWRERYEEDGIEGVKWNGQRGRPSKLTKSQKKALKRIILKGATSYGYPNELWTTFR
ITEIIRKEFKVKFHQDYVGILLHQLGFSYQKPKRIALERDEKAIQSWKRERWPDIKKAQKEGFEIIFLDESGISQNPYPAKTWGLIGKTPIIRHKMSWKK
LSVIGAISQKDFYFQVIEGSAKSQNLIHFLKMLLKENRKKILLVWDNLSADKSKTVNVFLQEHTKRLRVEFLPPYAPELNPQEYIWRRWKRNDMANFCPV
NLSSLFNRTKYTLRKLKSTTFCFESCWRQAGI

Blast result :

Comments

ISLbp6 is 60% aa similar to ISCARN39.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISLbp7 correspond to the second part of the transposase.

References

1] ISfinder submission (2018)
2] Bulach,D.M., Zuerner,R.L., Wilson,P., Seemann,T., McGrath,A., Cullen,P.A., Davis,J., Johnson,M., Kuczek,E., Alt,D.P., Peterson-Burch,B., Coppel,R.L., Rood,J.I., Davies,J.K. and Adler,B. (2006)
Proc. Natl. Acad. Sci. U.S.A. 103 (39), 14560-14565 .
3] Eslabao,M.R., Kremer,F.S., Ramos,R.T.J., Silva,A.L.C.,Azevendo,V.A.C., Pinto,L.S., Silva,E.F. and A,O.A.(2016) Direct GenBank submission.