ISLbp5

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP000348 ND Leptospira borgpetersenii
Leptospira borgpetersenii serovar Hardjo-bovis L550
DNA section
IS Length : 862 bp

Ends


IR Length : 15

IRL : GAGCCCGTCTCAAAACTGGTTGTACTTTTGAATCGTAATTATTTACTTGT
IRR : GAGCCCGTCTCAAAAATATCTATTAAAGAAATTGAAAACAATAGTGCTCG

Insertion site


Left flankDirect repeatRight flankDR Length
GATGGGGCGCCATATCATGATATGGCGCCCCA0

DNA sequence

GAGCCCGTCTCAAAACTGGTTGTACTTTTGAATCGTAATTATTTACTTGTGAAATGGACAAATATTATTCAGAGATCCCCGATGGACTTTGGGAAAAAAT
AGAACCATTAATCCCAAAAGTTAGGGCAAATCTCCAAGGAGGTCGCAATCGATTACCTACAAGAGTGGTAATGGCGGGGATCATCTATCGAATGAAAACA
GGCTGTCAGTGGCGGGCAATTCCGAGTAACTTCGGATCTGGTCAAACTTATCACAGAAGATTCCAAGAATGGGAAAGAGCGGGAGTATTCAAAAAGGTTT
ATAAATCTATTTTAAAATATTATGATGTGAAGAATAAGATAGCATGGGACTGGGCTTCGATGGATTCCGCAATTGTTAAAGCTCCCAAAGGGGGAGTTTA
ACCGGCAAAAACCCTACAGACCGCGCCAAATTAGGGGTTAGACGGCATATTCTTACGGATGGAAATGGAATTCCATTGGCCATAACATTGAGTGGAGCCA
ATGTTCATGACAAGCATAACGTAAAAGAGACTTTAAATTCAATCCTGATTTTTTCCGGTAGAAGAAAAAAGAAACCAAAACATCTCTGTCTTGATAAAGG
ATATGACTTTAAAGATACGGAAAAATTAATTAGAAGAAGAAATATCCGGCCTCACATTCGAAGAAGAGGCGAGAAACCATTGATCGGTAAATATAAAGGA
AAGCCTCGAAGATGGGTCGTTGAAAGAACGAACAGTTGGCACAATCGATTCAGGGCTATCCTTATCCGGTGGGAAAGAAAATCTGAAAATTATATGGCTT
CTCTTTACCTCGCGAGCACTATTGTTTTCAATTTCTTTAATAGATATTTTTGAGACGGGCTC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
348 bp115 aa54401+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDKYYSEIPDGLWEKIEPLIPKVRANLQGGRNRLPTRVVMAGIIYRMKTGCQWRAIPSNFGSGQTYHRRFQEWERAGVFKKVYKSILKYYDVKNKIAWDW
ASMDSAIVKAPKGGV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
473 bp157 aa381853+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SSQRGSLTGKNPTDRAKLGVRRHILTDGNGIPLAITLSGANVHDKHNVKETLNSILIFSGRRKKKPKHLCLDKGYDFKDTEKLIRRRNIRPHIRRRGEKP
LIGKYKGKPRRWVVERTNSWHNRFRAILIRWERKSENYMASLYLASTIVFNFFNRYF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
800 bp266 aa54853+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDKYYSEIPDGLWEKIEPLIPKVRANLQGGRNRLPTRVVMAGIIYRMKTGCQWRAIPSNFGSGQTYHRRFQEWERAGVFKKVYKSILKYYDVKNKIAWDW
ASMDSAIVKAPKGGSLTGKNPTDRAKLGVRRHILTDGNGIPLAITLSGANVHDKHNVKETLNSILIFSGRRKKKPKHLCLDKGYDFKDTEKLIRRRNIRP
HIRRRGEKPLIGKYKGKPRRWVVERTNSWHNRFRAILIRWERKSENYMASLYLASTIVFNFFNRYF

 

Blast result :
Comments
ISLbp5 is 69% aa similar to ISMba5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2018)
2] Bulach,D.M., Zuerner,R.L., Wilson,P., Seemann,T., McGrath,A., Cullen,P.A., Davis,J., Johnson,M., Kuczek,E., Alt,D.P., Peterson-Burch,B., Coppel,R.L., Rood,J.I., Davies,J.K. and Adler,B. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (39), 14560-14565 .