ISLhe63
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000517 | ND | Lactobacillus helveticus | Lactobacillus helveticus DPC 4571 |
DNA section
IS Length : 1846 bp
Ends
IR Length : 22/25
IRL : GTCAAAGTGTCTGGGAAATAACTAATTCTCGGACATGAAAAAAGAACGAT
IRR : GAAAAAGTGTCTGGGAAATAACCAACCCTTAGATACTGAAAAACGATCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATATTTATTTA | TGGTTAAACTAAG | 0 |
DNA sequence
GTCAAAGTGTCTGGGAAATAACTAATTCTCGGACATGAAAAAAGAACGATGATTTCCTATCGGATTTCATCGTTCTTTTTCTATACTTTAAGTGCCTTCC
TTGATATTATTGAATCACCACAAAACAAAAATGAAAGAAGGCAATCTCAATGTATCAAAATTATACCACAGGCCAAACTGCTTTAACACTTAATCTGGAC
TTTACTATCCCTAACAATCATTTAGCCAACACAATCAGCTGCTTCGTTGATTCTATTCCGGAAGATGTTTTGCTGGGTGATACCCCTAAAACTGGACGGC
CTGCTTATCATCCAGCTATGATGCTTAAGATTTTGCTTTTTGCATATTCCAGAAGAGTATTTTCAGGCAGAAAGATTGAATTAATGCTGGAAGAAAACCT
GCCTATGATGGTATTGGCAGAGCACCAAAAGATTTCATATCATACCATCAATAACTTTAGGTCTAGTGATCACGCCAATGAACTGGTTAAGAAAAGCTTC
CTCTACTTTACCAATTTGTTAGAAGCAGAAGGTCTAATCAATGAGGGCGCCATCTTTATTGATGGTACCAAGATTGAAGCTGACGCTAATCGCTATACTT
TTGTTTGGCGCAAGGCAGCTGAAAAGTTCCATGAAAAGCTCAAAGGCCAAGCAGATGAGCTTTATGATGAACTGATTGCCAAAGAAGTAGTCAAAGCAAT
GGCTCAAGAAAAGGTACAAACCAGTCAAGGCTTAGAAGAACTAGCGCAAGAAACAGAAGATGAGATTGAAAAGCTGACTAAAGAAATTGAGCAAGAGCCT
AAGGCAATTCCTGGCGGCTCGCTTAGAAAAGCAAGAAGACGTGGTCTTAAAAAGCTGCTTCATAGGCTAAAAAAAGACTATGTTCCTCGCATGAAGAAAT
ATGAAGAAGCAGAAGAAATCTTTGCTGGACGCAACAGCTATTCCAAGACTGATCATGATGCGACTTTTATGCATATGAAGGAAGACCATATGAAGAATGG
TCAGCTGAAGCCAGGCTACAATATTCAAGCCGCTACAACGGATCAATATGTAGTCGGCTTTGCCTTGTACCCTAACCCAACTGACTTTAAGACATTAGAA
CCATTTTTAAAGCAGATGCCAATATTAGATAAGTTCGATAAGATTGTCGCCGATGCTGGTTATGGCAGCGAGTACAACTATTCTATGCTAGAGAAAGAAT
ATCCAGACAAGAAGTACTATATTCCCTACACTATGTATGAGAAGGAAAAGACCAGAAAATATCGCAACGATCCGACTAAGCTGGCTAACTGGTTCTATGA
TGAAAAAGATGATTACTATCTTGATCAAAATGGCGTCAGGTTCAACTTTAAGTATTATAGTCAGCGTAAAGACCGCTCGACTGGTCAGGTTCGTGATTTT
AAGGTCTATGAAGCAGATGAATTCCAACTAACGCCAGAATTAGAACGCCTAGCCAAAACCAAAAGTGGTCGTCAACGCCAAGTACGCTATAATCCTAACT
GGCAGTATTTAAAGGAAAAAGCCAAAGAAGTTCTTCAGAGTCCTGAAGGCAGGCACATCTACAGCATGAGAAAGTATGATGTGGAGCCAATTTTTGGACA
TTTGAAGAACGTTTTTGGCATGCGTCGAACTCATTTAAGAGGCAAAAAGAAGGTTGAAACTGATGTTGGCATAGCCTTCATGATGATGAATTTAAGCAAA
TATTGGAACAGAAGATGGTCAAAAGACCAATTTTCTTTGTTCAAAAATAAAAATAGAGAGAAAAAACGACCAATCAAACTCAAATTAAGAGTTGAATTGA
TCGTTTTTCAGTATCTAAGGGTTGGTTATTTCCCAGACACTTTTTC
TTGATATTATTGAATCACCACAAAACAAAAATGAAAGAAGGCAATCTCAATGTATCAAAATTATACCACAGGCCAAACTGCTTTAACACTTAATCTGGAC
TTTACTATCCCTAACAATCATTTAGCCAACACAATCAGCTGCTTCGTTGATTCTATTCCGGAAGATGTTTTGCTGGGTGATACCCCTAAAACTGGACGGC
CTGCTTATCATCCAGCTATGATGCTTAAGATTTTGCTTTTTGCATATTCCAGAAGAGTATTTTCAGGCAGAAAGATTGAATTAATGCTGGAAGAAAACCT
GCCTATGATGGTATTGGCAGAGCACCAAAAGATTTCATATCATACCATCAATAACTTTAGGTCTAGTGATCACGCCAATGAACTGGTTAAGAAAAGCTTC
CTCTACTTTACCAATTTGTTAGAAGCAGAAGGTCTAATCAATGAGGGCGCCATCTTTATTGATGGTACCAAGATTGAAGCTGACGCTAATCGCTATACTT
TTGTTTGGCGCAAGGCAGCTGAAAAGTTCCATGAAAAGCTCAAAGGCCAAGCAGATGAGCTTTATGATGAACTGATTGCCAAAGAAGTAGTCAAAGCAAT
GGCTCAAGAAAAGGTACAAACCAGTCAAGGCTTAGAAGAACTAGCGCAAGAAACAGAAGATGAGATTGAAAAGCTGACTAAAGAAATTGAGCAAGAGCCT
AAGGCAATTCCTGGCGGCTCGCTTAGAAAAGCAAGAAGACGTGGTCTTAAAAAGCTGCTTCATAGGCTAAAAAAAGACTATGTTCCTCGCATGAAGAAAT
ATGAAGAAGCAGAAGAAATCTTTGCTGGACGCAACAGCTATTCCAAGACTGATCATGATGCGACTTTTATGCATATGAAGGAAGACCATATGAAGAATGG
TCAGCTGAAGCCAGGCTACAATATTCAAGCCGCTACAACGGATCAATATGTAGTCGGCTTTGCCTTGTACCCTAACCCAACTGACTTTAAGACATTAGAA
CCATTTTTAAAGCAGATGCCAATATTAGATAAGTTCGATAAGATTGTCGCCGATGCTGGTTATGGCAGCGAGTACAACTATTCTATGCTAGAGAAAGAAT
ATCCAGACAAGAAGTACTATATTCCCTACACTATGTATGAGAAGGAAAAGACCAGAAAATATCGCAACGATCCGACTAAGCTGGCTAACTGGTTCTATGA
TGAAAAAGATGATTACTATCTTGATCAAAATGGCGTCAGGTTCAACTTTAAGTATTATAGTCAGCGTAAAGACCGCTCGACTGGTCAGGTTCGTGATTTT
AAGGTCTATGAAGCAGATGAATTCCAACTAACGCCAGAATTAGAACGCCTAGCCAAAACCAAAAGTGGTCGTCAACGCCAAGTACGCTATAATCCTAACT
GGCAGTATTTAAAGGAAAAAGCCAAAGAAGTTCTTCAGAGTCCTGAAGGCAGGCACATCTACAGCATGAGAAAGTATGATGTGGAGCCAATTTTTGGACA
TTTGAAGAACGTTTTTGGCATGCGTCGAACTCATTTAAGAGGCAAAAAGAAGGTTGAAACTGATGTTGGCATAGCCTTCATGATGATGAATTTAAGCAAA
TATTGGAACAGAAGATGGTCAAAAGACCAATTTTCTTTGTTCAAAAATAAAAATAGAGAGAAAAAACGACCAATCAAACTCAAATTAAGAGTTGAATTGA
TCGTTTTTCAGTATCTAAGGGTTGGTTATTTCCCAGACACTTTTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1697 bp | 567 aa | 150 | 1846 | + | No |
Chemistry : DDE
ORF sequence :
MYQNYTTGQTALTLNLDFTIPNNHLANTISCFVDSIPEDVLLGDTPKTGRPAYHPAMMLKILLFAYSRRVFSGRKIELMLEENLPMMVLAEHQKISYHTI
NNFRSSDHANELVKKSFLYFTNLLEAEGLINEGAIFIDGTKIEADANRYTFVWRKAAEKFHEKLKGQADELYDELIAKEVVKAMAQEKVQTSQGLEELAQ
ETEDEIEKLTKEIEQEPKAIPGGSLRKARRRGLKKLLHRLKKDYVPRMKKYEEAEEIFAGRNSYSKTDHDATFMHMKEDHMKNGQLKPGYNIQAATTDQY
VVGFALYPNPTDFKTLEPFLKQMPILDKFDKIVADAGYGSEYNYSMLEKEYPDKKYYIPYTMYEKEKTRKYRNDPTKLANWFYDEKDDYYLDQNGVRFNF
KYYSQRKDRSTGQVRDFKVYEADEFQLTPELERLAKTKSGRQRQVRYNPNWQYLKEKAKEVLQSPEGRHIYSMRKYDVEPIFGHLKNVFGMRRTHLRGKK
KVETDVGIAFMMMNLSKYWNRRWSKDQFSLFKNKNREKKRPIKLKLRVELIVFQYLRVGYFPDTFSG
NNFRSSDHANELVKKSFLYFTNLLEAEGLINEGAIFIDGTKIEADANRYTFVWRKAAEKFHEKLKGQADELYDELIAKEVVKAMAQEKVQTSQGLEELAQ
ETEDEIEKLTKEIEQEPKAIPGGSLRKARRRGLKKLLHRLKKDYVPRMKKYEEAEEIFAGRNSYSKTDHDATFMHMKEDHMKNGQLKPGYNIQAATTDQY
VVGFALYPNPTDFKTLEPFLKQMPILDKFDKIVADAGYGSEYNYSMLEKEYPDKKYYIPYTMYEKEKTRKYRNDPTKLANWFYDEKDDYYLDQNGVRFNF
KYYSQRKDRSTGQVRDFKVYEADEFQLTPELERLAKTKSGRQRQVRYNPNWQYLKEKAKEVLQSPEGRHIYSMRKYDVEPIFGHLKNVFGMRRTHLRGKK
KVETDVGIAFMMMNLSKYWNRRWSKDQFSLFKNKNREKKRPIKLKLRVELIVFQYLRVGYFPDTFSG
Blast result :
Comments
ISLhe63 is 97% aa similar to ISLac1.
The STOP codon is done by the insertion site.
The STOP codon is done by the insertion site.
References
1] ISfinder submission (2018)
2] Callanan,M., Kaleta,P., O'Callaghan,J., O'Sullivan,O., Jordan,K., McAuliffe,O., Sangrador-Vegas,A., Slattery,L., Fitzgerald,G.F., Beresford,T. and Ross,R.P. (2008) J. Bacteriol. 190 (2), 727-735.
2] Callanan,M., Kaleta,P., O'Callaghan,J., O'Sullivan,O., Jordan,K., McAuliffe,O., Sangrador-Vegas,A., Slattery,L., Fitzgerald,G.F., Beresford,T. and Ross,R.P. (2008) J. Bacteriol. 190 (2), 727-735.