ISLhe66

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP000517 ND Lactobacillus helveticus
Lactobacillus helveticus DPC 4571
DNA section
IS Length : 2262 bp

Ends


IR Length : 26

IRL : GTAAGCGTCCAAAACTGAAGTATCTTTTTTGGCTCCTAATTTTCGGCTAT
IRR : GTAAGCGTCCAAAACTGAAGTATCTTAAAACCCCATCTTGCCTTGTGCTA

Insertion site


Left flankDirect repeatRight flankDR Length
AAAGAAAAGTCTAACTAAAAAGTAAGAATTAAA8

DNA sequence

GTAAGCGTCCAAAACTGAAGTATCTTTTTTGGCTCCTAATTTTCGGCTATCCTTGTTTCATAAACTTAAGGAGGCAACGAATATGCTTGAAAAACAACAG
TCAATCGTGGCTCTGGATCATCCGAAAAGACCAAAAAAGGCACGTCCGCCACGCAACAATTTGATGCTTAGTCTGAAAAAGAATGACCTTGAACTGAATT
TTTATAGCTCTTTGGATCCTGATGGCCTAAACAAAATCTTGAGCAAGGTCCTATCATGATTAACCTTTATGACTTGGGGCAGGTCTACATTGTTTGTGGT
CGAACAGATATGAGACGCGGAATCGATGGCCTGGCTGCCATAGTTAAAGATGAATTTGATTTGGATCCTTTTTCTGGCCGAGTCTTTATCTTCTGCGGTG
GCAGTCGCGACCGTTTCAAGGCTTTATATTGGGATGGTCAGGGATATTGGCTGCTTTATAAAAGATTTGAAAATGGCAAGATGATCTGGCCTAACAATCA
GGATGAAGTCAAAGCTTTGTCAAAAGAACAAGTAGATTGGTTAATGAAGGGCTTTTCTATTGAGCCTGGGATAAAAGCAACGAACGCACGCAATTTCTAT
TGATTTTGCGTGCGTTTCTGGTATGCTTTAAATTATCAAAAAATGAGAGAGATCCTAATGACAAGAGCTGAAACAAAAGATGAAATCATTAAAGGCTTAA
TGACTACTATTAACAACCTGAACGAAGAGATTTCCTTCTTGCGTGAACAAGTAGCCTACTTAACGCAAAAACGTTACGGCAAATCATCAGAACAAACGCC
TTTATCAGGCCAAACCAGCTTATTTGATGAAGAAAATTTTGCGGGCGATGAAGACGAGGATCTTCCCTGTTGAAACTGAAGAAATTGCCTATCGCAGAAG
AAGACGCAAGGCTAAGAGAGCCAATGTCTTAAAAGCTTTTGCCAGTGAAGAAATTCATCACCAATTGACTGGCAAAGATCTGCTTTGCCCAGATTGCCAG
CAGGAAATGAAAGAGATCGGCTCTTGTCCGGTTCGCCAAGAGCTGCTGTTCATTCCAGCACAAATCAAGCGACTGGATCATATCCAACATGCTTACAAAT
GTCAGTGCTGCAGTTTAAAAAATACTAGTGATAAGATTGTCAAGGCACCAGTGCCTAAAGCACCGCTAGCTCATAGCTATGGCTCTGCTTCAGTCATTGC
TCATACCATCTATCAAAAGTATGAGTTGAAAGTACCAGCCTATCGGCAAAAAAACGACTGGCAGAAAATGGGTCTGCCTATTACGCGCCGGGATATCATC
AACTGGCAGATCAAATCTACGGAATATTACTTTAAGCCGCTTTATCAATTGCTTAAGAAAAAGCTGCTAGAGCAAAAGTTTTTACACGCAGATGAAACAA
CTTATCGCGTCTTGGAAAGCAAGACGCAAAAAACTTTCTACTGGACTTTTCTGTCTGACAAGCAGGCAGAAAAGCCAATTACTCTATATCACCATAATCC
GCATCGCAGCGGTCAAGTAGCGATCCAATTCTTAGGCAATTATGCTGGCTACTTGCATTGTGACATGTGGCAGGCGTATGGGCAATTGGCGCAAGCTACT
TTGGTTGGCTGCTGGGCTCATGTCAGAAGAAAGTTCTTTGAAGCTGTGCCGCCTAAAGCATCAGACAAATCAATATCTAAACAAGGCGTGCGCTACTGCA
ATAGAATGTTTGCCCTAGAAAAAGACTGGGGAAATTTGTCTAACGAAGAGCGTCTTCGATTGCGTCGAAAGAAACTAGCGCCGCTGATGAGGGAATTCTT
TAACTGGTGTCGTAAGCAGAAAACAAGGACGCTGCCTAATTCCAAGCTAGGAAAAGCAATTGCTTACTCTTTGAATCATGAAGAAACATTTAAACATGTT
TTGTTAGATGGCCGACTGGTTTTGTCCAACAATCTAGCTGAGCGTGCAATCAAATCGCTGGTTATCGGCCGCAAGAACTGGTTGTTCTCTCAGAGTTTTA
ATGGTGCCCAATCTTCGGCCATCATTCTTAGTTTGATTGAAACTGCCAAGCGCAATGGCTTGGATCCAGAAAAATATCTGGTATATCTACTCTCTAATTT
GCCCAACGAATCAACTCTGACAGATAAAGAAGCACTGTCAGCTTATTTGCCATGGGAAAAAGCGGCACAAGCAAACTGCAGATAAAATTTGAATACAAAG
GCAACCCCATCTTAGCACAAGGCAAGATGGGGTTTTAAGATACTTCAGTTTTGGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
177 bp58 aa83259+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MLEKQQSIVALDHPKRPKKARPPRNNLMLSLKKNDLELNFYSSLDPDGLNKILSKVLS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp114 aa256603+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MINLYDLGQVYIVCGRTDMRRGIDGLAAIVKDEFDLDPFSGRVFIFCGGSRDRFKALYWDGQGYWLLYKRFENGKMIWPNNQDEVKALSKEQVDWLMKGF
SIEPGIKATNARNFY

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1543 bp514 aa6432185+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MREILMTRAETKDEIIKGLMTTINNLNEEISFLREQVAYLTQKRYGKSSEQTPLSGQTSLFDEENFAGDEDEDLPPVETEEIAYRRRRRKAKRANVLKAF
ASEEIHHQLTGKDLLCPDCQQEMKEIGSCPVRQELLFIPAQIKRLDHIQHAYKCQCCSLKNTSDKIVKAPVPKAPLAHSYGSASVIAHTIYQKYELKVPA
YRQKNDWQKMGLPITRRDIINWQIKSTEYYFKPLYQLLKKKLLEQKFLHADETTYRVLESKTQKTFYWTFLSDKQAEKPITLYHHNPHRSGQVAIQFLGN
YAGYLHCDMWQAYGQLAQATLVGCWAHVRRKFFEAVPPKASDKSISKQGVRYCNRMFALEKDWGNLSNEERLRLRRKKLAPLMREFFNWCRKQKTRTLPN
SKLGKAIAYSLNHEETFKHVLLDGRLVLSNNLAERAIKSLVIGRKNWLFSQSFNGAQSSAIILSLIETAKRNGLDPEKYLVYLLSNLPNESTLTDKEALS
AYLPWEKAAQANCR

 

Blast result :
Comments
ISLhe66 is 59% aa similar to ISCth11.
Possible sequencing error inducing unexpected frameshift in the transposase (in silico reconstructed).
References
1] ISfinder submission (2018)
2] Callanan,M., Kaleta,P., O'Callaghan,J., O'Sullivan,O., Jordan,K., McAuliffe,O., Sangrador-Vegas,A., Slattery,L., Fitzgerald,G.F., Beresford,T. and Ross,R.P. (2008) J. Bacteriol. 190 (2), 727-735.