ISfinder Mirror Site

ISLhe61

Family IS3
Group IS150

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP000517	ND	Lactobacillus helveticus	Lactobacillus helveticus DPC 4571

DNA section

IS Length : 1468 bp

Ends

IR Length : 22/25

IRL : TGATATGAACCCCGAAATTCGGACAAGAATTTCGAGGTTTTTGTTATGTC
IRR : TGATATGAACCCCAAATTTTGGACATATTATTGGACTGCTTTATTTAAGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TAATCTAAGC	ATA	GATTTGAGCT	3

DNA sequence

TGATATGAACCCCGAAATTCGGACAAGAATTTCGAGGTTTTTGTTATGTCCAAATTATCTAAACAAGACAAAATTGATATTTACAATAGCTGGAAAAATT
ATGGTAAATCAGTTGAGCAATTAGGTAGAGAATACAATGTTACTTCTGGTAATTTAAGATATGTATTAAGCTTAATGGAGCGTTATGGAGTAAAAGTTCT
AGATAAGCCATACGAAAATTATGCAACAGAGTTCAAAGAAAAAGCCATTAAAAGAGTCCTTTTTGCTCATGAATCAGCTCGCCGAGTATCATTAGAGCTA
GGTTTATCCAATGGTGGACAAATTCATAATTGGATTCGCAAATATAAAGAAGATGGGTATAATGTCATTAATCACAAGAAAGGCAGACCTACCCATGAAG
AACAAAGACAAGCAGATAGAGGAGCTTCAAAGGCAGATAAAAGACCTAAAACAGAAGAACTTAAGACTTACTGTCGAGAACGAGTTTGTAAAAAAATTGA
ACGCCTTAGTCTCCCAAAGAACCAAAAACCAACAGCACAAGAAATAGCTACTACGGTTACTCAGCTAAGGCAGGAACTTCACGTAACCGTTAAGTTTGTG
CTTGATACGATCAATGCCAATCCTGATCTGCCACATCTGTCGCGCAGTAATTATTACTACACTTTGACTAAAGATGACAAAAATTTAAAAAACGAAAAAG
TTATGAAGCGGATTAAAGAAATATTCGAAGAGCACAGACACCGGTATGGTTACCGCAGAATTACTGCTCAATTGCATCGAGAAGGTATGAGAGTGAATCA
TAAGCGCGTCAAGCGATTAATGGTCAAAATGCATTTATATGCCATTACCATCAGACGTAGATTTAAATATTCAAGCTATAAAGGAACTATTGGAAAAATC
AAGTCCAATTTAATTAAACGTCATTTTGCGGCGATTATCCCAGATAGGCATTGGTACTCAGATATTACTGAATTTCACCTTAATGGAGAAAAGCTTTATC
TATCGCCTGTGATGGACGGCTGCAGTCAAGAGATTGTTGCTTATACATTAAGCCGTCATCCAGTATTGAAACAAGTTATGGATATGCTGGATTTAGCTTT
ATAAGAAACATCCAGCTCTTAATGGCCTAATTTTTCATACTGATCAAGGCTGGCAATATCAGCACGCTGCATTCCAAGCTTGGCTTAAAAATCATGGTGT
TTCTCAATCTATGTCACGCAAAGGCAATTCTTTAGATGATGGCTTAATGGAAGGGTTTTTTGGTATTCTTAAGCGTGAGATGTTCTATGGCTTTGAAAAA
ACATTCAAAACCATGGATGAATTAGAGCAAGCAATCAAAGAATACATTCATTACTACAATCATGATAGAATCAAAACGGTACTAAAAAACCATACTCCGA
TAGAATATCGAAATATGGTCTTAAATAAAGCAGTCCAATAATATGTCCAAAATTTGGGGTTCATATCA

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
1059 bp	350 aa	46	1104	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSKLSKQDKIDIYNSWKNYGKSVEQLGREYNVTSGNLRYVLSLMERYGVKVLDKPYENYATEFKEKAIKRVLFAHESARRVSLEGLSNGGQIHNWIRKYK
EDGYNVINHKKGRPTHEEQRQADRGASKADKRPKTEELKTYCRERVCKKIERLSLPKNQKPTAQEIATTVTQLRQELHVTVKFLDTINANPDLPHLSRSN
YYYTLTKDDKNLKNEKVMKRIKEIFEEHRHRYGYRRITAQLHREGMRVNHKRVKRLMVKMHLYAITIRRRFKYSSYKGTIGKIKSNLIKRHFAAIIPDRH
WYSDITEFHLNGEKLYLSPVMDGCSQEIVAYTLSRHPVLKQVMDMLDLAL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
345 bp	114 aa	1097	1441	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LYKKHPALNGLIFHTDQGWQYQHAAFQAWLKNHGVSQSMSRKGNSLDDGLMEGFFGILKREMFYGFEKTFKTMDELEQAIKEYIHYYNHDRIKTVLKNHT
PIEYRNMVLNKAVQ

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1396 bp	464 aa	46	1441	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

Blast result :

Comments

ISLhe61 is 74% aa similar to ISL6.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder submission (2018)
2] Callanan,M., Kaleta,P., O'Callaghan,J., O'Sullivan,O., Jordan,K., McAuliffe,O., Sangrador-Vegas,A., Slattery,L., Fitzgerald,G.F., Beresford,T. and Ross,R.P. (2008) J. Bacteriol. 190 (2), 727-735.