ISLhe60
- Family IS607
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000517 | ND | Lactobacillus helveticus | Lactobacillus helveticus DPC 4571 |
DNA section
IS Length : 2293 bp
Ends
IR Length : 0
IRL : AGTCATAAATTATTAGTTTGATTATTTCATAAATAATCATTTGATATGAT
IRR : GAAAATATTAGGTCAATACAGTCATTTTCCTTCAAATGACCCTATTTTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACAACATGATGT | TAATAT | GGAAGACTTTAT | 6 |
DNA sequence
AGTCATAAATTATTAGTTTGATTATTTCATAAATAATCATTTGATATGATTGATTATTTTTAAGTCTTGCTTTAATGACTTTATGAGTATATATGATATA
ATATTAGTGAAGGAGATGATCTTTTGAAAGCTGGAAAAGTGATGGATTTGCTTCAAATCAGTCGTTCAACTCTGAAACGCTATCGTGAAAAAGGAATCCT
TGAGGCTAAAAAATTGCCAACCGGTCAATATGATTTTGATGATGATTCCGTTTTTTTGCTTAAGAATAAACATTCCAAACGCTTGACCGTTTTATATGGC
CGAGTTTCAACCTATAAGCAGAAAGCTGATTTGGCTAATCAAATGCAGGAGCTGTCTGATTTTGCCCAAAGCAAAGGCTTTCAGGCAAACGATAGCTTTC
AAGATATTGCCAGTGGCATTTCTTTCAAGAACCGAAAGCAGTTTTTCAAGATGCTTGACTTAATCTTAGCAGGCAAGGTCAAGCGGGTTATTATCACTCG
CAAAGATCGCTTAAGTCGGGTGGGCTTTGAACTGTTTGAATATTTATTTCAAAACTACGGTACTGAAATTGACGTGATTTCAGATGAGATGAATCCCAAA
ACTGATGAGCAGGAATTGTTTGAAGAAATCATTAGCCTGCTTCACTGCTTTTCAATGAGAGAATATTCCCATCGCAGAGCTGAAAGAAAGCTTTTTGAAG
CCACTTTGAATCAAAAGGAAAAGACTAAGTAGACTAATCATATTTTAAAGGATGCGAAACTATGCGTAAGAAAATCGTGCCTAGAAACAAGGCTAAATAT
GAACATCTAGCCAGCGAATACCTGGTTGCTGGAAACAGCAAGCTGGATCAGTGCAGTCGTGCCAGTCATGATCTTTATAACCGTGCCCTTTATGATCTAC
GTCAGGGCTTCTTTAAAAGAAATTACGTCAAAGGCTATTGCGATTTAGACCAAAGATTCAAAAAGCGCTATAGCGCTCGTGAATCTATGCTTTACCACTC
ATTATTCTATGTTCAAAGCGCCCAACAGACTCTAAAAGAAGTCAATACTATCTGGTCGGCTTGGCTTAAGGCACTCAAAGCATATCAAGCCAACCCCGGC
AAATTTACCGGTCGGCCAAGAATGCCTAAGTACTTAGCCAAAGGTCAAAGACATGTCTTCTTTGTCACTAACCAAAACGCCAAAGTAAAGGATGACTATT
TAATCATACCTAAGCTTGGCTTCAAACTGAAATTAACACCTCACCTCAAGGCTATTCAAAGAGTAGCTTTTAAGCCGGTCTATGGCGGTTATAAGGCCAT
CGTGCAATACAAAACAAATCGAGATATTGACTATTTGCCTGATAACGGTCGCTACATGGGTATTGATCCGGGAGTAGACAATGCCTTTGCTTGCGTGGCT
AATATCCAAAAAGCGCCTTTGCTTGTTAATGGTCGGGCCATCAAATCGGTTAATCAGTACTACAACAAGGAGCGTTCAAGGCTAAAAGCTTTGCAGGCTC
GGTATCATCAGCTTGAAAGCATAGTTGATACCCAGCAGGGAAAGAAGCCAGTTTATCGGGAGACCAAAGCCATGCGCAGAATTACGGCATGGCGCAATCG
CAAGATCCGGCAGTTTGCCCACAAAGCATCCAAGCGCATCGTTGATTATGCGCTAAGCTGTGGTGCAAACACGATCGTGATCGGCAATAACAAGTCTTGG
AAGCGCAGTTCCGATATGGGCAGAAAGAACAATCAAAACTTTATCGGCATTCCGCACAAGGTCATGATCGACATGATTCAATATAAAGCCAACCTAGCCG
GCATTAGCGTCATTAGAACTAATGAAAGCTACACTAGTCAGACAAGTGCTTTGGATCATGAAAAGCCTTGCTGGGAAAACGGCAGCCAGAGTCGTAAAAG
ACAAGGCAAAACCCCGATTAACAGACGCATTCACCGTGGGCTGTTCCAGTCGAATAACGGCAGACTGGTTAATGCGGATATTAACGGAGCCTTGCAGATA
ATCAGAAAAGTATTCCCTAAATTTTCATTAGATGAAGGGATAGAGGATGCAGTGTTGCATCCAGTCAAATGGTCGCCTCTCATTTGAGCGTCCACTTGAC
AAACGAAAATAGCTTGGCGACTGGCTTTAGCTGGGTTGACGCTGTTGTAAAGTTGTCCTTATAGCTTCGGTTATTATCATAGATAAACGGAGTGTTTTAA
AAAGGTTAACGGCAGATATTGGCTAAAACAAGTTGCATTCTTAGGAAAATAGGGTCATTTGAAGGAAAATGACTGTATTGACCTAATATTTTC
ATATTAGTGAAGGAGATGATCTTTTGAAAGCTGGAAAAGTGATGGATTTGCTTCAAATCAGTCGTTCAACTCTGAAACGCTATCGTGAAAAAGGAATCCT
TGAGGCTAAAAAATTGCCAACCGGTCAATATGATTTTGATGATGATTCCGTTTTTTTGCTTAAGAATAAACATTCCAAACGCTTGACCGTTTTATATGGC
CGAGTTTCAACCTATAAGCAGAAAGCTGATTTGGCTAATCAAATGCAGGAGCTGTCTGATTTTGCCCAAAGCAAAGGCTTTCAGGCAAACGATAGCTTTC
AAGATATTGCCAGTGGCATTTCTTTCAAGAACCGAAAGCAGTTTTTCAAGATGCTTGACTTAATCTTAGCAGGCAAGGTCAAGCGGGTTATTATCACTCG
CAAAGATCGCTTAAGTCGGGTGGGCTTTGAACTGTTTGAATATTTATTTCAAAACTACGGTACTGAAATTGACGTGATTTCAGATGAGATGAATCCCAAA
ACTGATGAGCAGGAATTGTTTGAAGAAATCATTAGCCTGCTTCACTGCTTTTCAATGAGAGAATATTCCCATCGCAGAGCTGAAAGAAAGCTTTTTGAAG
CCACTTTGAATCAAAAGGAAAAGACTAAGTAGACTAATCATATTTTAAAGGATGCGAAACTATGCGTAAGAAAATCGTGCCTAGAAACAAGGCTAAATAT
GAACATCTAGCCAGCGAATACCTGGTTGCTGGAAACAGCAAGCTGGATCAGTGCAGTCGTGCCAGTCATGATCTTTATAACCGTGCCCTTTATGATCTAC
GTCAGGGCTTCTTTAAAAGAAATTACGTCAAAGGCTATTGCGATTTAGACCAAAGATTCAAAAAGCGCTATAGCGCTCGTGAATCTATGCTTTACCACTC
ATTATTCTATGTTCAAAGCGCCCAACAGACTCTAAAAGAAGTCAATACTATCTGGTCGGCTTGGCTTAAGGCACTCAAAGCATATCAAGCCAACCCCGGC
AAATTTACCGGTCGGCCAAGAATGCCTAAGTACTTAGCCAAAGGTCAAAGACATGTCTTCTTTGTCACTAACCAAAACGCCAAAGTAAAGGATGACTATT
TAATCATACCTAAGCTTGGCTTCAAACTGAAATTAACACCTCACCTCAAGGCTATTCAAAGAGTAGCTTTTAAGCCGGTCTATGGCGGTTATAAGGCCAT
CGTGCAATACAAAACAAATCGAGATATTGACTATTTGCCTGATAACGGTCGCTACATGGGTATTGATCCGGGAGTAGACAATGCCTTTGCTTGCGTGGCT
AATATCCAAAAAGCGCCTTTGCTTGTTAATGGTCGGGCCATCAAATCGGTTAATCAGTACTACAACAAGGAGCGTTCAAGGCTAAAAGCTTTGCAGGCTC
GGTATCATCAGCTTGAAAGCATAGTTGATACCCAGCAGGGAAAGAAGCCAGTTTATCGGGAGACCAAAGCCATGCGCAGAATTACGGCATGGCGCAATCG
CAAGATCCGGCAGTTTGCCCACAAAGCATCCAAGCGCATCGTTGATTATGCGCTAAGCTGTGGTGCAAACACGATCGTGATCGGCAATAACAAGTCTTGG
AAGCGCAGTTCCGATATGGGCAGAAAGAACAATCAAAACTTTATCGGCATTCCGCACAAGGTCATGATCGACATGATTCAATATAAAGCCAACCTAGCCG
GCATTAGCGTCATTAGAACTAATGAAAGCTACACTAGTCAGACAAGTGCTTTGGATCATGAAAAGCCTTGCTGGGAAAACGGCAGCCAGAGTCGTAAAAG
ACAAGGCAAAACCCCGATTAACAGACGCATTCACCGTGGGCTGTTCCAGTCGAATAACGGCAGACTGGTTAATGCGGATATTAACGGAGCCTTGCAGATA
ATCAGAAAAGTATTCCCTAAATTTTCATTAGATGAAGGGATAGAGGATGCAGTGTTGCATCCAGTCAAATGGTCGCCTCTCATTTGAGCGTCCACTTGAC
AAACGAAAATAGCTTGGCGACTGGCTTTAGCTGGGTTGACGCTGTTGTAAAGTTGTCCTTATAGCTTCGGTTATTATCATAGATAAACGGAGTGTTTTAA
AAAGGTTAACGGCAGATATTGGCTAAAACAAGTTGCATTCTTAGGAAAATAGGGTCATTTGAAGGAAAATGACTGTATTGACCTAATATTTTC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 609 bp | 202 aa | 124 | 732 | + | No |
Chemistry : Serine
ORF sequence :
MKAGKVMDLLQISRSTLKRYREKGILEAKKLPTGQYDFDDDSVFLLKNKHSKRLTVLYGRVSTYKQKADLANQMQELSDFAQSKGFQANDSFQDIASGIS
FKNRKQFFKMLDLILAGKVKRVIITRKDRLSRVGFELFEYLFQNYGTEIDVISDEMNPKTDEQELFEEIISLLHCFSMREYSHRRAERKLFEATLNQKEK
TK
FKNRKQFFKMLDLILAGKVKRVIITRKDRLSRVGFELFEYLFQNYGTEIDVISDEMNPKTDEQELFEEIISLLHCFSMREYSHRRAERKLFEATLNQKEK
TK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1326 bp | 441 aa | 762 | 2087 | + | No |
AG : TnpB
ORF sequence :
MRKKIVPRNKAKYEHLASEYLVAGNSKLDQCSRASHDLYNRALYDLRQGFFKRNYVKGYCDLDQRFKKRYSARESMLYHSLFYVQSAQQTLKEVNTIWSA
WLKALKAYQANPGKFTGRPRMPKYLAKGQRHVFFVTNQNAKVKDDYLIIPKLGFKLKLTPHLKAIQRVAFKPVYGGYKAIVQYKTNRDIDYLPDNGRYMG
IDPGVDNAFACVANIQKAPLLVNGRAIKSVNQYYNKERSRLKALQARYHQLESIVDTQQGKKPVYRETKAMRRITAWRNRKIRQFAHKASKRIVDYALSC
GANTIVIGNNKSWKRSSDMGRKNNQNFIGIPHKVMIDMIQYKANLAGISVIRTNESYTSQTSALDHEKPCWENGSQSRKRQGKTPINRRIHRGLFQSNNG
RLVNADINGALQIIRKVFPKFSLDEGIEDAVLHPVKWSPLI
WLKALKAYQANPGKFTGRPRMPKYLAKGQRHVFFVTNQNAKVKDDYLIIPKLGFKLKLTPHLKAIQRVAFKPVYGGYKAIVQYKTNRDIDYLPDNGRYMG
IDPGVDNAFACVANIQKAPLLVNGRAIKSVNQYYNKERSRLKALQARYHQLESIVDTQQGKKPVYRETKAMRRITAWRNRKIRQFAHKASKRIVDYALSC
GANTIVIGNNKSWKRSSDMGRKNNQNFIGIPHKVMIDMIQYKANLAGISVIRTNESYTSQTSALDHEKPCWENGSQSRKRQGKTPINRRIHRGLFQSNNG
RLVNADINGALQIIRKVFPKFSLDEGIEDAVLHPVKWSPLI
Blast result :
Comments
ISLhe60 is 73% (transposase) aa similar to ISCbo10.
There is an empty site in Lactobacillus helveticus strain CAUH18 (CP012381.1).
There is an empty site in Lactobacillus helveticus strain CAUH18 (CP012381.1).
References
1] ISfinder submission (2018)
2] Callanan,M., Kaleta,P., O'Callaghan,J., O'Sullivan,O., Jordan,K., McAuliffe,O., Sangrador-Vegas,A., Slattery,L., Fitzgerald,G.F., Beresford,T. and Ross,R.P. (2008) J. Bacteriol. 190 (2), 727-735.
2] Callanan,M., Kaleta,P., O'Callaghan,J., O'Sullivan,O., Jordan,K., McAuliffe,O., Sangrador-Vegas,A., Slattery,L., Fitzgerald,G.F., Beresford,T. and Ross,R.P. (2008) J. Bacteriol. 190 (2), 727-735.