ISEch5
- Family IS200/IS605
- Group IS200
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002038 | ND | Dickeya dadantii | Dickeya dadantii 3937 |
DNA section
IS Length : 711 bp
Ends
Left end : GTCTATAGAAAACCCCCAGCTAGGCTGGGGGTTCCGTAAAGCTTCCAGCTTTGGGGCGGATATAAAAACCCCTTTTGATTTGTTAAAACACCTTGCGGTC II struct. : Yes
Right end : ATGCAAATGTCAGATTGCGATGCGCCTGTTAGGGCGCGGCTGGTAACAGAGCCTTATAGGCGCATATGAAAAACCTCCGGCTATGCCGGAGGATATTTAT II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CCTGATGGCGGGTTTT | TATT | TTTGTATCTTTCCCA | TTAT |
DNA sequence
GTCTATAGAAAACCCCCAGCTAGGCTGGGGGTTCCGTAAAGCTTCCAGCTTTGGGGCGGATATAAAAACCCCTTTTGATTTGTTAAAACACCTTGCGGTC
TGGCAACTGCAATGGTCAAACAAGAAATCAAAAGGGGGTCCCAATGGGGGACGAAAAGAGCTTAGCGCACACCCGCTGGAACTGTAAATATCACATAGTT
TTCGCACCCAAATACCGAAGACAGGTCTTCTACGGTGAGAAACGGCGGGTAGTAGGGAGTATCTTAAGAAAGCTGTGTGAATGGAAAAATGTGCAGATTC
TGGAGGCGGAGTGCTGTGCTGATCACATACATATGCTTGTGGAGATCCCGCCGAAGATGAGTGTATCGAGCGTCATGGGGTATCTGAAAGGCAAAAGCAG
TCTGATGCTTTATGAACAATTTGGTGATCTGAAATTCAAATATAGGAACAGGGAGTTTTGGTGCCGAGGGTATTATGTAGATACGGTGGGTAAAAATACC
TCGAAGATCCGGGACTACATAAAGCATCAACTGGAGCAGGATAAACTGGGCGAACAGTTATCAATCCCGTATCCAGGTAGCCCGTTTACGGGCCGTAAGT
AACGGATTTGGATGCAAATGTCAGATTGCGATGCGCCTGTTAGGGCGCGGCTGGTAACAGAGCCTTATAGGCGCATATGAAAAACCTCCGGCTATGCCGG
AGGATATTTAT
TGGCAACTGCAATGGTCAAACAAGAAATCAAAAGGGGGTCCCAATGGGGGACGAAAAGAGCTTAGCGCACACCCGCTGGAACTGTAAATATCACATAGTT
TTCGCACCCAAATACCGAAGACAGGTCTTCTACGGTGAGAAACGGCGGGTAGTAGGGAGTATCTTAAGAAAGCTGTGTGAATGGAAAAATGTGCAGATTC
TGGAGGCGGAGTGCTGTGCTGATCACATACATATGCTTGTGGAGATCCCGCCGAAGATGAGTGTATCGAGCGTCATGGGGTATCTGAAAGGCAAAAGCAG
TCTGATGCTTTATGAACAATTTGGTGATCTGAAATTCAAATATAGGAACAGGGAGTTTTGGTGCCGAGGGTATTATGTAGATACGGTGGGTAAAAATACC
TCGAAGATCCGGGACTACATAAAGCATCAACTGGAGCAGGATAAACTGGGCGAACAGTTATCAATCCCGTATCCAGGTAGCCCGTTTACGGGCCGTAAGT
AACGGATTTGGATGCAAATGTCAGATTGCGATGCGCCTGTTAGGGCGCGGCTGGTAACAGAGCCTTATAGGCGCATATGAAAAACCTCCGGCTATGCCGG
AGGATATTTAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 459 bp | 152 aa | 144 | 602 | + | No |
Chemistry : Y1
ORF sequence :
MGDEKSLAHTRWNCKYHIVFAPKYRRQVFYGEKRRVVGSILRKLCEWKNVQILEAECCADHIHMLVEIPPKMSVSSVMGYLKGKSSLMLYEQFGDLKFKY
RNREFWCRGYYVDTVGKNTSKIRDYIKHQLEQDKLGEQLSIPYPGSPFTGRK
RNREFWCRGYYVDTVGKNTSKIRDYIKHQLEQDKLGEQLSIPYPGSPFTGRK
Blast result :
Comments
ISEch5 is 98% aa similar to IS200F.
References
1] ISfinder submission (2018)
2] Glasner,J.D., Yang,C.H., Reverchon,S., Hugouvieux-Cotte-Pattat,N., Condemine,G., Bohin,J.P., Van Gijsegem,F., Yang,S., Franza,T., Expert,D., Plunkett,G. III, San Francisco,M.J., Charkowski,A.O., Py,B., Bell,K., Rauscher,L., Rodriguez-Palenzuela,P., Toussaint,A., Holeva,M.C., He,S.Y., Douet,V., Boccara,M., Blanco,C., Toth,I., Anderson,B.D., Biehl,B.S., Mau,B., Flynn,S.M., Barras,F., Lindeberg,M., Birch,P.R., Tsuyumu,S., Shi,X., Hibbing,M., Yap,M.N., Carpentier,M., Dassa,E., Umehara,M., Kim,J.F., Rusch,M., Soni,P., Mayhew,G.F., Fouts,D.E., Gill,S.R., Blattner,F.R., Keen,N.T. and Perna,N.T. (2011) J. Bacteriol. 193 (8), 2076-2077.
2] Glasner,J.D., Yang,C.H., Reverchon,S., Hugouvieux-Cotte-Pattat,N., Condemine,G., Bohin,J.P., Van Gijsegem,F., Yang,S., Franza,T., Expert,D., Plunkett,G. III, San Francisco,M.J., Charkowski,A.O., Py,B., Bell,K., Rauscher,L., Rodriguez-Palenzuela,P., Toussaint,A., Holeva,M.C., He,S.Y., Douet,V., Boccara,M., Blanco,C., Toth,I., Anderson,B.D., Biehl,B.S., Mau,B., Flynn,S.M., Barras,F., Lindeberg,M., Birch,P.R., Tsuyumu,S., Shi,X., Hibbing,M., Yap,M.N., Carpentier,M., Dassa,E., Umehara,M., Kim,J.F., Rusch,M., Soni,P., Mayhew,G.F., Fouts,D.E., Gill,S.R., Blattner,F.R., Keen,N.T. and Perna,N.T. (2011) J. Bacteriol. 193 (8), 2076-2077.