ISDet3
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001924 | ND | Dehalococcoides mccartyi | Dehalococcoides mccartyi GT |
DNA section
IS Length : 1210 bp
Ends
IR Length : 27/40
IRL : TGACCTGACCCCCTAAAACGAGTCCAGTCTTAATGTTAGACTGTGACCGA
IRR : TGACCTGCCCCCATTAGTTGTACCACTTGTTAAGTTAGTACAGCGCTCAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATATATCCTG | GCAT | TGTTTAAGGA | 4 |
DNA sequence
TGACCTGACCCCCTAAAACGAGTCCAGTCTTAATGTTAGACTGTGACCGAAAAGAAAGGGGACTAGATGCCTAGAAAAGCTTTTACCGCGGAACAGATCA
TCAACAAGCTTCGTGAAGCCGAGCTGATTCTGAATCAGGGCACAACGGTGCCTGAAGCCTGCCGAAAGATTAGAGTTACCGATCAGACCTACTATCGATG
GCGCAAGGAATATGGCGGCTTAAACGTCGACCAGGCCAAGCGCCTTAAGGACCTGGAAAAAGAAAACGTCCAGCTAAAGCGCCTTGTGGCCGATCTTTCA
CTCGATAAGGCCATATTGAAGGAGGCGTCCCGGGGAAACTCCTAAGCCCGTCCCAAAGGCGACAAGTGATAGCCAAAGTGCGTATCAGCCTTGGAATCTC
TGAAAGACGGGCTTGTCAGGTGCTGGGTCAAGCCAGATCAACACAGCGCCGTAAAGAATCGATTACCGACGACGAAGCGAAGCTTACGCTACGAATAATT
GAACTGGCCAGCAAATACGGACGATACGGGTATCGGAGAATCACGGCGATTTTGCAAATAGCTGGTTGGAGAGTGAATCACAAAAGAGTCGAACGGATCT
GGAGAAAGGAAGGGCTGAAGGTGCCCCAGAAGCAACCCAAGCGCGGTAGACTATGGCTAAATGATGGTTCCTGCATCAGGTTGCGCCCCGAGCATAAGGA
TCATGTTTGGAGCTACGATTTCGTGGCCGAGAGGACGGTAAACGGCCGGGCGTTCCGGATATTGAATATCATTGATGAGTACACTCGCGAGTGCCTGGCG
ATGAAGATTGGCCGGCGGATCACCTCCCAGGAGGTCATTGATAAGCTGTTCGAGCTATTCCTCTTGAGAGGCATCCCGGAAAACATCAGGTCGGACAACG
GCCCGGAATTCACGGCCAAGCAAATCAGGAAATGGCTGAATAGGATCGGTGTAAATACTTTGTTCATTGAGCCGGGAAGCCCCTGGGAGAACGGTTACAT
AGAATCATTCAACGGGAAACTGAGAGACGAACTGCTCAACCGGGAAATATTTTTTACCCTTACCGAGGCAAAAGTATTGATTGAAGAATGGAGGAAAGAC
TACAATCAAATACGCCCGCACAGCGCATTGGGTTACAGACCACCGGCTCCAGAGGCCGTAATGAGCGCTGTACTAACTTAACAAGTGGTACAACTAATGG
GGGCAGGTCA
TCAACAAGCTTCGTGAAGCCGAGCTGATTCTGAATCAGGGCACAACGGTGCCTGAAGCCTGCCGAAAGATTAGAGTTACCGATCAGACCTACTATCGATG
GCGCAAGGAATATGGCGGCTTAAACGTCGACCAGGCCAAGCGCCTTAAGGACCTGGAAAAAGAAAACGTCCAGCTAAAGCGCCTTGTGGCCGATCTTTCA
CTCGATAAGGCCATATTGAAGGAGGCGTCCCGGGGAAACTCCTAAGCCCGTCCCAAAGGCGACAAGTGATAGCCAAAGTGCGTATCAGCCTTGGAATCTC
TGAAAGACGGGCTTGTCAGGTGCTGGGTCAAGCCAGATCAACACAGCGCCGTAAAGAATCGATTACCGACGACGAAGCGAAGCTTACGCTACGAATAATT
GAACTGGCCAGCAAATACGGACGATACGGGTATCGGAGAATCACGGCGATTTTGCAAATAGCTGGTTGGAGAGTGAATCACAAAAGAGTCGAACGGATCT
GGAGAAAGGAAGGGCTGAAGGTGCCCCAGAAGCAACCCAAGCGCGGTAGACTATGGCTAAATGATGGTTCCTGCATCAGGTTGCGCCCCGAGCATAAGGA
TCATGTTTGGAGCTACGATTTCGTGGCCGAGAGGACGGTAAACGGCCGGGCGTTCCGGATATTGAATATCATTGATGAGTACACTCGCGAGTGCCTGGCG
ATGAAGATTGGCCGGCGGATCACCTCCCAGGAGGTCATTGATAAGCTGTTCGAGCTATTCCTCTTGAGAGGCATCCCGGAAAACATCAGGTCGGACAACG
GCCCGGAATTCACGGCCAAGCAAATCAGGAAATGGCTGAATAGGATCGGTGTAAATACTTTGTTCATTGAGCCGGGAAGCCCCTGGGAGAACGGTTACAT
AGAATCATTCAACGGGAAACTGAGAGACGAACTGCTCAACCGGGAAATATTTTTTACCCTTACCGAGGCAAAAGTATTGATTGAAGAATGGAGGAAAGAC
TACAATCAAATACGCCCGCACAGCGCATTGGGTTACAGACCACCGGCTCCAGAGGCCGTAATGAGCGCTGTACTAACTTAACAAGTGGTACAACTAATGG
GGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated Yes
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 67 | 345 | + | No |
Description : First part of the transposase
ORF sequence :
MPRKAFTAEQIINKLREAELILNQGTTVPEACRKIRVTDQTYYRWRKEYGGLNVDQAKRLKDLEKENVQLKRLVADLSLDKAILKEASRGNS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 873 bp | 290 aa | 309 | 1181 | + | No |
Description : Second part of the transposase
ORF sequence :
GHIEGGVPGKLLSPSQRRQVIAKVRISLGISERRACQVLGQARSTQRRKESITDDEAKLTLRIIELASKYGRYGYRRITAILQIAGWRVNHKRVERIWRK
EGLKVPQKQPKRGRLWLNDGSCIRLRPEHKDHVWSYDFVAERTVNGRAFRILNIIDEYTRECLAMKIGRRITSQEVIDKLFELFLLRGIPENIRSDNGPE
FTAKQIRKWLNRIGVNTLFIEPGSPWENGYIESFNGKLRDELLNREIFFTLTEAKVLIEEWRKDYNQIRPHSALGYRPPAPEAVMSAVLT
EGLKVPQKQPKRGRLWLNDGSCIRLRPEHKDHVWSYDFVAERTVNGRAFRILNIIDEYTRECLAMKIGRRITSQEVIDKLFELFLLRGIPENIRSDNGPE
FTAKQIRKWLNRIGVNTLFIEPGSPWENGYIESFNGKLRDELLNREIFFTLTEAKVLIEEWRKDYNQIRPHSALGYRPPAPEAVMSAVLT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1115 bp | 371 aa | 67 | 1181 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRKAFTAEQIINKLREAELILNQGTTVPEACRKIRVTDQTYYRWRKEYGGLNVDQAKRLKDLEKENVQLKRLVADLSLDKAILKEASRGKLLSPSQRRQ
VIAKVRISLGISERRACQVLGQARSTQRRKESITDDEAKLTLRIIELASKYGRYGYRRITAILQIAGWRVNHKRVERIWRKEGLKVPQKQPKRGRLWLND
GSCIRLRPEHKDHVWSYDFVAERTVNGRAFRILNIIDEYTRECLAMKIGRRITSQEVIDKLFELFLLRGIPENIRSDNGPEFTAKQIRKWLNRIGVNTLF
IEPGSPWENGYIESFNGKLRDELLNREIFFTLTEAKVLIEEWRKDYNQIRPHSALGYRPPAPEAVMSAVLT
VIAKVRISLGISERRACQVLGQARSTQRRKESITDDEAKLTLRIIELASKYGRYGYRRITAILQIAGWRVNHKRVERIWRKEGLKVPQKQPKRGRLWLND
GSCIRLRPEHKDHVWSYDFVAERTVNGRAFRILNIIDEYTRECLAMKIGRRITSQEVIDKLFELFLLRGIPENIRSDNGPEFTAKQIRKWLNRIGVNTLF
IEPGSPWENGYIESFNGKLRDELLNREIFFTLTEAKVLIEEWRKDYNQIRPHSALGYRPPAPEAVMSAVLT
Blast result :
Comments
ISDet3 is 96% aa similar to ISDsp2.
The third ORF is the putative ORFAB tran,sposase reconstricted in silico by possible -1 frameshift.
The third ORF is the putative ORFAB tran,sposase reconstricted in silico by possible -1 frameshift.
References
1] ISfinder annotation (2018)
2] Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D., Goodwin,L., Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Kuske,C.R., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Ritalahti,K.M., Fletcher,K.E., Sung,Y., Wagner,D., Konstantinidis,K.T., Loeffler,F.E. and Woyke,T. (2010) Direct GenBank submission.
2] Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D., Goodwin,L., Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Kuske,C.R., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Ritalahti,K.M., Fletcher,K.E., Sung,Y., Wagner,D., Konstantinidis,K.T., Loeffler,F.E. and Woyke,T. (2010) Direct GenBank submission.