ISfinder Mirror Site

ISChy9

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP000141	ND	Carboxydothermus hydrogenoformans	Carboxydothermus hydrogenoformans Z-2901

DNA section

IS Length : 2059 bp

Ends

Left end : CGAAAAACGGCCGCCGCAGGCCCCGCCCTTTAGGGCTGGGGATAAGGCGGCCAAATTTTTATGGGCTACAATTATATTGCCGCTTGTGGTATAATAATTT II struct. : No
Right end : CGTGATGCTGTAGCGCCGTCTCATGGCGGTAGCGAGAGCCCGGGAGAGGTCGCGGTGGTTCCCCTCAGAACCCCCGGGCTTTAGCCCTGGGGAGGCTCAG II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TGTATAATATAGGCAAAT	TGAC	AAAAGAGGTAATATCGCGA	TCAG

DNA sequence

CGAAAAACGGCCGCCGCAGGCCCCGCCCTTTAGGGCTGGGGATAAGGCGGCCAAATTTTTATGGGCTACAATTATATTGCCGCTTGTGGTATAATAATTT
TATGAAGCGCAAATTTAAGTCCAACAACAATATCATGTATTCTTGCAAATACCATGTAGTCTGGTGCCCGAAATACCGGCGTCCCGTCCTGAAAGATGGA
GTAGACGTGCGGCTCAAAGAGATCCTCTTTGAAGTGGCACGGGAGAAAAGGGCCGAAATTATTGAATTGGAGGTCATGCCGGACCACGTCCACCTGCTGG
TAGAGGTGGACCCGCAGTTCGGCATCCACCGGCTGGTGAAGCTCATGAAGGGCCGGTCCTCCCGCATCCTACGCCAGGAGTTCCCGTGGCTCAGGTCGAG
GCTGCCGACCCTCTGGACCAACAGCTACTTCGTGGCTACCGTTGGAGGTGCGCCCCGTGCCACCCTACGCGAGTACATCGAGAAGCAAAAGTACGTCTAG
CACGCCCAGCTTCGTGTGCGAGCTCCTCCTGCGGGTCTCTCCCGCCCAGGAGAAAGCCTTGCTGGCCCGCCTGGAGGCAGCCCGGCAGCTCTACAACGCC
TGCCTGGGGGAAGCTTTAAAGCGCGTGGGGCTCGTGCAACAATCGAAGCTGTGGCAGAAAGCCTGGAAAGCAGGGGACAAAGAGCTAAGAAGGGCCCTGA
TCCGGGAAGCTTGTCTAAAGTACGGTTTCACGGACGCCGCTTTGCAGCACTACGCCGTGAAGATACGGCGCTCCTGCTGGATAGGCGACCACCTGGATGT
CCACGTGGCCCAGAAGCTGGGCACCAGGGCCTTCCGTGCCGCCCAAAGGGTGCTTTTCGGACAGGCACGCAAGGTGCGCTTCAAGGGCAAGAACCAGATG
GACACCGTGGAGGGCAAGAGCAACGGAGCGGGCATCCGGTGGCGGAATGGTCGTGTGGTCTGGAGGGGCCTGGTTCTACCAGCCATTATCGACCCAGAAG
AACCGGTAATCTGTCATGCTCTTTCCTGCCGGGTCAAGTACGTCCGCCTGGTGAGGCGAAAAATAAACGGCCGCAACCGATTCTACGCGCAGCTTATCTG
CGAGGGCACACCCTACCAGAAACCTGAAAATGCCCTGGGAGAAGGGACTGTCGGCATCGACGCAGGGCCCTCTATCATCGCCCGTGTCAACAGTAAAGAG
GCGCGCCTGGATCGCTTTTGCGAGGAGCTTGCAAAGAAGGAAGCAGAAATACGCAGGCTCCAGCGCAGGCTGGACCGTTCCCGCAGAGCCAACAACCCTG
AGAACTACAACCCCGACGGTACGGTAAGGAAAGGGCCGAAGCAGTGGAAGAAGACCAACACGTACCTCAAGACCGAGGCCCGCCTGGCGGAGCTCTGGCG
CAGGCTGGCCGCCCACCGCAGGAGCCTCATTGGCAGGATGGTCAACGAGACGCTGCGCATGGGCAACGTGTTCAAATTTGAAAAGCTTTCCTACCGCGCC
CTGCAGAGGATGTTTGGCAAGTCCGTCAACACCCGGGCGCCGGGGATGTTCTTCCGGGAGCTCACCCGCAAGGCTGAGAGTGCTGGCGGGAAGGTCCTGG
AGTTTCCCACCCGGCTTAAAGTCAAAGGTACAGAAACTTGCGTTGGCCTTTCCAGCTACTGCGTTTGCGGGGAACACAAGAAGAAGTCTTTGTCCGAGCG
CTGGCACATATGCTCATGCGGTGTAAAGTGCCAGCGGGACCTCTACAGCGCCTACCTGGCATGTTTTGTGGAACAGGTTGGGCAGAATGATTTCGAGCTC
GACGCGGGCACTGCCGCGAGGCAGTGGCCGGGTGCGGAGCCGCTCCTGCAAGCGGCGTCGGGCCGTATCCCCAACCCCCGAATGTCCGGCTCCTCCGGAC
AGCCCCGCAAGGGGACAGAGGGGGTCGCTGCGGAAGAGAGGACAGCCAAAGCCGAGGCCCGTGATGCTGTAGCGCCGTCTCATGGCGGTAGCGAGAGCCC
GGGAGAGGTCGCGGTGGTTCCCCTCAGAACCCCCGGGCTTTAGCCCTGGGGAGGCTCAG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
417 bp	138 aa	84	500	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MWYNNFMKRKFKSNNNIMYSCKYHVVWCPKYRRPVLKDGVDVRLKEILFEVAREKRAEIIELEVMPDHVHLLVEVDPQFGIHRLVKLMKGRSSRILRQEF
PWLRSRLPTLWTNSYFVATVGGAPRATLREYIEKQKYV

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1587 bp	528 aa	457	2043	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MPPYASTSRSKSTSSTPSFVCELLLRVSPAQEKALLARLEAARQLYNACLGEALKRVGLVQQSKLWQKAWKAGDKELRRALIREACLKYGFTDAALQHYA
VKIRRSCWIGDHLDVHVAQKLGTRAFRAAQRVLFGQARKVRFKGKNQMDTVEGKSNGAGIRWRNGRVVWRGLVLPAIIDPEEPVICHALSCRVKYVRLVR
RKINGRNRFYAQLICEGTPYQKPENALGEGTVGIDAGPSIIARVNSKEARLDRFCEELAKKEAEIRRLQRRLDRSRRANNPENYNPDGTVRKGPKQWKKT
NTYLKTEARLAELWRRLAAHRRSLIGRMVNETLRMGNVFKFEKLSYRALQRMFGKSVNTRAPGMFFRELTRKAESAGGKVLEFPTRLKVKGTETCVGLSS
YCVCGEHKKKSLSERWHICSCGVKCQRDLYSAYLACFVEQVGQNDFELDAGTAARQWPGAEPLLQAASGRIPNPRMSGSSGQPRKGTEGVAAEERTAKAE
ARDAVAPSHGGSESPGEVAVVPLRTPGL

Blast result :

Comments

ISChy9 is 86% (transposase) aa similar to ISClte2.

References

1] ISfinder submission (2018)
2] Wu,M., Ren,Q., Durkin,A.S., Daugherty,S.C., Brinkac,L.M., Dodson,R.J., Madupu,R., Sullivan,S.A., Kolonay,J.F., Haft,D.H., Nelson,W.C., Tallon,L.J., Jones,K.M., Ulrich,L.E., Gonzalez,J.M., Zhulin,I.B., Robb,F.T. and Eisen,J.A. (2005) PLoS Genet. 1 (5), E65 and PLoS Genet. 2006 Apr;2(4):e60. Haft, Daniel H [added].