ISfinder Mirror Site

ISSeq1

Family ISL3
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISSzo1

Accession number	Transposition	Origin	Host
NC_012470	ND	Streptococcus equi	Streptococcus equi subsp. zooepidemicus H70 Streptococcus equi equi 4047 Streptococcus equi zooepidemicus 35246

DNA section

IS Length : 1415 bp

Ends

IR Length : 21/25

IRL : GGCTCTATAATTTCTGTAGTGGGTAAGTCCACTGCAGGGGTTATAGGGCT
IRR : GGCTCTATGTCAACTGTAGTGGGTAATTGACAAGCTAACATCTGGAGAGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAGGGGGTAA	TGATTATA	CCGTTAAAT	8
CAATTACCCA	GCTTTTTA	TAGTATTAT	8
CTCTTTAGAC	GTTAGTTA	TTAGTCAAC	8
CTTCAATGGT	TAAAGTTT	TGGACTAAC	8
AATCTCTTGA	CAAATAAA	AAAGGGGTG	8
TCATCACCTA	CCTTTTTT	AACCTCCTG	8
CTTTTGATTT		TATGATTTTA	0
CTCAGTCCAA	CAAAAAAG	CGCCCAAGAT	8
CACACAAAAA		CCAAAAAAAG	0
AAAGATGACA	ATAAAAAA	TAACCGTCTT	8
GCCATTCCAA	AGTATATG	TCATTCTAAT	8
ATTCAACTAA	CAAAAAGC	AAGAGGACAT	8
AAATTAGCAA	TACAAAAA	TGCCTACCAG	8
AAATAAATAC		CAAAAATTCT	0
AGTAAACCAT	TAATTTAC	TGTTTCTTAA	8
CATTCTAGTA	AAAAAGAA	TACCTAGCTT	8
ATTTAAGACT	AGATTACA	CTGCTCTTTG	8
GTGGAAATCA		AAACTCCGCC	0
GTGGAAATCA		AAACTCCGCC	0
ATGGAAATAA		AGAATCCTGAT	0
CCATTAGCCA	AAAAGTAC	TCACCCTCTAT	8
CAATTACCCA	GCTTTTTA	TAGTATTATAA	8
CAGAAATCGT	CTTTTAAT	AATGCCTAAAT	8

DNA sequence

GGCTCTATAATTTCTGTAGTGGGTAAGTCCACTGCAGGGGTTATAGGGCTTTATGAGTATCAAAAAAGTCCCATATGACCTATAATGAAAAGCGACTAAA
CTATCATTTAGAAAGACTCATATGGAACAATCACATGTTATCACACAACTACTGGGAATTAAAGACCCTCATATCACATTCTCCAAAGAAATACACGACA
TGAAAACTCATAAGGAATTGAAAGCTGTCCTTGACTACGATGCCCCACCTTGCCCTAACTGCCAAAGTCAGATGGGCAAGTACGACTTCCAACGGGAATC
CAAGGTCCCTTTTCTCGATTGCGCAGGCTACAAGACTTTGATTCGCCTCAAAAAGCGCCGTTTCAAATGTCAGTTTTGCGGAAAAATTACTGTCGCTGAG
ACTTCTCTAGTCCCTAAAAACCATCAAATACCAACCATCGTCAAACAGAAGGTAGCCCAGCTTCTCATTGAGAAAGTCTCCATGACCGCTATCGCTGATA
GACTATCCATCTCCACCTCAACCGTCATGCGAAAGCTCAACGAGTTCACGTTCAAGCCTCATTTGACTTATTTACCCGAACATATGTCCTGGGATGAATA
TGCCTTTAAGAAGGGCAAGATGAGCTTTATTGCTCAGGACTTTGACACCAACATCATCATCGCTATTTTGGATGGACGGACGAAAGCTGTCATTCGCAAC
CACTTCCTGAGATATCCTCGGCAGGTCAGAAACGACGTTAAACTCATCACCATGGATATGTTTACCCCCTATTACAACCTGGCTAAGATGCTTTTCCCAA
ACGCTCAGATTGTCCTTGATCGTTTCCACATTGTGCAACATTTGGGACGTGCCATGAACCGTATCCGTACTCAAATCATGAACTCTTTTGATCGAAAATC
CCACGAATACAAGGCTTTGAAACGTTACTGGAAGCTGATTCAACAAGATAGCAGCAAACTCAGTGACAAGCGTTTTTACCGCCCGACTTTTCGCATGCAT
TTGACAAACAAGGAGGTGGTCGAGCGTCTTTTGAGCTACTCTGACGAGCTTAGAAAGCATTATGACCTTTATCAGCTTCTGCTTTTTCACTTCCAGGAAA
AGCAAGCTGACCACTTTTTCGGCCTGATTGAGGAGCAGATAGACAGCAGCAATCCTCTCTTTCAGACCGTTTTCAAGACCTTTTTAAAAGACAGAGACAA
GATTGAGAACGCACTGGATTTGCCTTATTCTAACGCTAAACTGGAGGCTACCAATAATCTCATCAAAGTCATCAAGCGCAATGCTTTTGGATTTCGGAAC
TTTGAAAACTTTAAAAGACGGATTTTGATCGCTATCAATATGAAAAAAGAGAAGACCAAATTGGTCCTCTCCAGATGTTAGCTTGTCAATTACCCACTAC
AGTTGACATAGAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1260 bp	419 aa	122	1381	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MEQSHVITQLLGIKDPHITFSKEIHDMKTHKELKAVLDYDAPPCPNCQSQMGKYDFQRESKVPFLDCAGYKTLIRLKKRRFKCQFCGKITVAETSLVPKN
HQIPTIVKQKVAQLLIEKVSMTAIADRLSISTSTVMRKLNEFTFKPHLTYLPEHMSWDEYAFKKGKMSFIAQDFDTNIIIAILDGRTKAVIRNHFLRYPR
QVRNDVKLITMDMFTPYYNLAKMLFPNAQIVLDRFHIVQHLGRAMNRIRTQIMNSFDRKSHEYKALKRYWKLIQQDSSKLSDKRFYRPTFRMHLTNKEVV
ERLLSYSDELRKHYDLYQLLLFHFQEKQADHFFGLIEEQIDSSNPLFQTVFKTFLKDRDKIENALDLPYSNAKLEATNNLIKVIKRNAFGFRNFENFKRR
ILIAINMKKEKTKLVLSRC

Blast result :

Comments

ISSeq1 is 86% aa similar to ISSth1.

References

1] ISfinder submission (2012)
2] Holden,M.T., Heather,Z., Paillot,R., Steward,K.F., Webb,K., Ainslie,F., Jourdan,T., Bason,N.C., Holroyd,N.E., Mungall,K., Quail,M.A., Sanders,M., Simmonds,M., Willey,D., Brooks,K., Aanensen,D.M., Spratt,B.G., Jolley,K.A., Maiden,M.C., Kehoe,M., Chanter,N., Bentley,S.D., Robinson,C., Maskell,D.J., Parkhill,J. (2009) PLoS Pathog. 5 (3), E1000346 .