ISPrre1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY090559 | ND | Providencia rettgeri | Providencia rettgeri |
DNA section
IS Length : 1249 bp
Ends
IR Length : 22/26
IRL : TGTAGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACC
IRR : TGTAGTGGTCAACTAAAATTGGCCACGGTTTTAGAGTTTTTCCGGTATTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAGCGATGC | CTT | TCTTATTTGA | 3 |
DNA sequence
TGTAGTGGTTTAATAAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCCTTAATGAAAAAACGAAATTTCAGTGCAGAAT
TCAGACGTGAATCAGCCCAGCTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGT
AAAGCAATTACGGGACGAACGGGCAGGCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGAAGAAAAAAATTCAACGT
ATTGAAATGGAAAACGAAATATTAAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGGTCTCGGTGATCGGGAAACTCAGAGCGCATTATCC
TGTGGCCACTCTTTGCTGCGTGTTCGGAGTTCACCGCAGCAGCTATAGATACCGGGAAAACCGGCCTGACAATCCGGACGGCAGGAGAGCCGTATTACGT
AGTCAGGTTCAGGAGCTGCACGGCCTCAGTCATAGCTCAGCAGGTGCAAGAAGTATCGCTGTAATGGCAACACACAGGGGCTTCCGGATGGGACGATGGC
TTGCCGGACGGCTAATGAAGGAGATGGGGCTGGTGAGCTGTCAGCAGCCTGTTCACCGGTATAAACGTGGCGGTCATGAACACATTGCTATCCCGAACCA
CCTTGAGCGACAGTTCGCAGTGACAGAGCCCAATCAGGTATGGTGCGGCGACGTAACGTATATCTGGACCGGTAAACGCTGGGCATACCTGGCCGTTGTA
CTCGACCTGTTCGCAAGGAAACCCGTGGGCTGGGCAATGTCATTTTCTCCGGACAGCAAACTGACAACCAAAGCGCTGAAAATGGCATGGGAAATACGAA
ATAAGCCATCCGGGCTCATGTTCCACAGTGATCAGGGTAGCCACTATACAAGCAGGCAGTTCCGACAGTTACTGTGGCGATACCGGATAAAACAAAGTAT
GAGTAGGCGTGGTAACTGCTGGGATAACAGCCCGATGGAGCGCTTCTTCAGAAGTCTGAAAAATGAGTGGGTGCCGGTGACCGGCTACATCAGCTTCAGT
GAAGCAGCCCATGCAATAACGGATTATATCGTCGGGTATTACAGCGAAGTCAGGCCGCATGAATATAACGGTGGATTACCACCAAACGAATCAGAAAACC
AATACCGGAAAAACTCTAAAACCGTGGCCAATTTTAGTTGACCACTACA
TCAGACGTGAATCAGCCCAGCTGGTTGTGGATCAGAACTATACAGTTGCAGATGCCGCGAAAGCCATGAATGTCGGGCTTTCCACCTTGACGCGGTGGGT
AAAGCAATTACGGGACGAACGGGCAGGCAAAACACCGAAAGCATCCCCTATCACGCCGGAACAAATTGAGATACGTGAGCTGAAGAAAAAAATTCAACGT
ATTGAAATGGAAAACGAAATATTAAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGGTCTCGGTGATCGGGAAACTCAGAGCGCATTATCC
TGTGGCCACTCTTTGCTGCGTGTTCGGAGTTCACCGCAGCAGCTATAGATACCGGGAAAACCGGCCTGACAATCCGGACGGCAGGAGAGCCGTATTACGT
AGTCAGGTTCAGGAGCTGCACGGCCTCAGTCATAGCTCAGCAGGTGCAAGAAGTATCGCTGTAATGGCAACACACAGGGGCTTCCGGATGGGACGATGGC
TTGCCGGACGGCTAATGAAGGAGATGGGGCTGGTGAGCTGTCAGCAGCCTGTTCACCGGTATAAACGTGGCGGTCATGAACACATTGCTATCCCGAACCA
CCTTGAGCGACAGTTCGCAGTGACAGAGCCCAATCAGGTATGGTGCGGCGACGTAACGTATATCTGGACCGGTAAACGCTGGGCATACCTGGCCGTTGTA
CTCGACCTGTTCGCAAGGAAACCCGTGGGCTGGGCAATGTCATTTTCTCCGGACAGCAAACTGACAACCAAAGCGCTGAAAATGGCATGGGAAATACGAA
ATAAGCCATCCGGGCTCATGTTCCACAGTGATCAGGGTAGCCACTATACAAGCAGGCAGTTCCGACAGTTACTGTGGCGATACCGGATAAAACAAAGTAT
GAGTAGGCGTGGTAACTGCTGGGATAACAGCCCGATGGAGCGCTTCTTCAGAAGTCTGAAAAATGAGTGGGTGCCGGTGACCGGCTACATCAGCTTCAGT
GAAGCAGCCCATGCAATAACGGATTATATCGTCGGGTATTACAGCGAAGTCAGGCCGCATGAATATAACGGTGGATTACCACCAAACGAATCAGAAAACC
AATACCGGAAAAACTCTAAAACCGTGGCCAATTTTAGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 73 | 375 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRNFSAEFRRESAQLVVDQNYTVADAAKAMNVGLSTLTRWVKQLRDERAGKTPKASPITPEQIEIRELKKKIQRIEMENEILKKATALLMSDSLNRSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 936 bp | 311 aa | 306 | 1241 | + | No |
Description : Second part of the transposase
ORF sequence :
NGKRNIKKGYRALDVRLPEQVSVIGKLRAHYPVATLCCVFGVHRSSYRYRENRPDNPDGRRAVLRSQVQELHGLSHSSAGARSIAVMATHRGFRMGRWLA
GRLMKEMGLVSCQQPVHRYKRGGHEHIAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSKLTTKALKMAWEIRNK
PSGLMFHSDQGSHYTSRQFRQLLWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYISFSEAAHAITDYIVGYYSEVRPHEYNGGLPPNESENQY
RKNSKTVANFS
GRLMKEMGLVSCQQPVHRYKRGGHEHIAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSKLTTKALKMAWEIRNK
PSGLMFHSDQGSHYTSRQFRQLLWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYISFSEAAHAITDYIVGYYSEVRPHEYNGGLPPNESENQY
RKNSKTVANFS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1169 bp | 389 aa | 73 | 1241 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRNFSAEFRRESAQLVVDQNYTVADAAKAMNVGLSTLTRWVKQLRDERAGKTPKASPITPEQIEIRELKKKIQRIEMENEILKKGYRALDVRLPEQVS
VIGKLRAHYPVATLCCVFGVHRSSYRYRENRPDNPDGRRAVLRSQVQELHGLSHSSAGARSIAVMATHRGFRMGRWLAGRLMKEMGLVSCQQPVHRYKRG
GHEHIAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSKLTTKALKMAWEIRNKPSGLMFHSDQGSHYTSRQFRQL
LWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYISFSEAAHAITDYIVGYYSEVRPHEYNGGLPPNESENQYRKNSKTVANFS
VIGKLRAHYPVATLCCVFGVHRSSYRYRENRPDNPDGRRAVLRSQVQELHGLSHSSAGARSIAVMATHRGFRMGRWLAGRLMKEMGLVSCQQPVHRYKRG
GHEHIAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSKLTTKALKMAWEIRNKPSGLMFHSDQGSHYTSRQFRQL
LWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYISFSEAAHAITDYIVGYYSEVRPHEYNGGLPPNESENQYRKNSKTVANFS
Blast result :
Comments
References
1] Dongsheng Zhou (2018) Direct submission.
2] Boltner,D., MacMahon,C., Pembroke,J.T., Strike,P. and Osborn,A.M. (2002) J. Bacteriol. 184 (18), 5158-5169.
3] Pembroke,J.T., MacMahon,C. and McGrath,B. (2002) Cell. Mol. Life Sci. 59 (12), 2055-2064.
2] Boltner,D., MacMahon,C., Pembroke,J.T., Strike,P. and Osborn,A.M. (2002) J. Bacteriol. 184 (18), 5158-5169.
3] Pembroke,J.T., MacMahon,C. and McGrath,B. (2002) Cell. Mol. Life Sci. 59 (12), 2055-2064.