ISPa91

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MF168946 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa
Pseudomonas aeruginosa strain FFUP_PS_CB5
DNA section
IS Length : 1289 bp

Ends


IR Length : 27/33

IRL : TGAGGTTGCCCCTCAAAAGTGGAGTTCTTAGCTTTGTCCAAAATGTAGAC
IRR : TGATGTTGCCCCTGTTTTGTGGAGTTCTTAGCTCCCTGCTTACGCGGCTT

Insertion site


Left flankDirect repeatRight flankDR Length
TGGCCTTGCAGACCGACAAGGGC3

DNA sequence

TGAGGTTGCCCCTCAAAAGTGGAGTTCTTAGCTTTGTCCAAAATGTAGACAAGGAGCTAAGCAATGGTGGCAAAGCAAGTACGAGCGGCATACACGCAGG
AATTTAGGTTGGAGGCTGTACGCTTGGTGCAAAGCGGGCAATCTCAGATGCAGGTGTGCCAGACGCTGGGAATCCCCAAGGCCAGCTTGGGCAGTTGGGT
GCGACAGGCAAAAGTAGGCACCCTCAAGCTTGGGACTGCTGATGGTGCTGGCAGCAAGGTAACGCCGGAGCAAATGGAGATAAGCCGTCTGCGAGCCGAA
GTTGCACGTTTGCGAATGGAGCGTGACATCGCAAAAAAAGCGGCGGCGTACTTCGCGCAGGACGTGCTGCGAGGTATGCCTGGATTGAGCAAATGAAGAA
CGTCTATCCAATTGCTGTTTCTTGCCATGTTCTGCAAGTGAGCAGCAGTGGTTACTTCCGCTTCAGACGCAGCGATGGTGAAGCATTACACCTATGCAGT
AATGAATTGGCCCTGGCCCACATACGTGCCATTCATGCAGAGGTTAAAGGCGAATACGGCTGGCCACGCATACACAAAGAGCTGCAAGCTAGAGGTCTGC
GTATTGGCAAAGAGCGGGTGCGTAAGCTCATGGCCGAGCACGGCATACGGGCTAAAACCAAGCGCAAGTTTGTTGTGACCACAGACAGCAAGCACAGCCT
GCCGGTTGCTCCTGACTTGGTGCAGCGGCGTTTTACGCCGCAGGCACCGAACCAACTTCTGTGCGGCGACATCACATACATCCAGACCGACGAAGGCTGG
TTGTACTTGGCTGCAGTGATTGACATGTTCAGCCGTCAGGTGGTGGGATGGAGCCTGCAGCCCCACATGCAAAGCAGCTTGGTTAAAGATGCCATGGCAA
TGGCTTGGTGGCGAAGGCGGCCTGAACCGGGAGCCATATTCCACAGCGACAGGGGCAGCCAATATTGCAGCCAGAGCTTCCAGGCCACATTAGCAGGCTG
GGGCATACGCTCGTCCATGAGCCGCAAAGGCAATTGTTGGGACAACTCGCCCATCGAAAGTTTCTGGGGTCGATTGAAGACCGCTTGCGTCCACGGCCAA
CGCTTCGCTACCCGGGAGCACGCAAGGCAGGCCATCATGAACTGGATGGCCTTCTACAATTACCGCAGATTACATTCGAGCTTGGGCTACCTCAGCCCCA
TGCAATACGAGCAACGCTGGTACGAGGCACAGCGCAAAAAAGCCGCGTAAGCAGGGAGCTAAGAACTCCACAAAACAGGGGCAACATCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
333 bp110 aa64396+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MVAKQVRAAYTQEFRLEAVRLVQSGQSQMQVCQTLGIPKASLGSWVRQAKVGTLKLGTADGAGSKVTPEQMEISRLRAEVARLRMERDIAKKAAAYFAQD
VLRGMPGLSK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
924 bp307 aa3271250+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

HRKKSGGVLRAGRAARYAWIEQMKNVYPIAVSCHVLQVSSSGYFRFRRSDGEALHLCSNELALAHIRAIHAEVKGEYGWPRIHKELQARGLRIGKERVRK
LMAEHGIRAKTKRKFVVTTDSKHSLPVAPDLVQRRFTPQAPNQLLCGDITYIQTDEGWLYLAAVIDMFSRQVVGWSLQPHMQSSLVKDAMAMAWWRRRPE
PGAIFHSDRGSQYCSQSFQATLAGWGIRSSMSRKGNCWDNSPIESFWGRLKTACVHGQRFATREHARQAIMNWMAFYNYRRLHSSLGYLSPMQYEQRWYE
AQRKKAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1187 bp395 aa641250+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVAKQVRAAYTQEFRLEAVRLVQSGQSQMQVCQTLGIPKASLGSWVRQAKVGTLKLGTADGAGSKVTPEQMEISRLRAEVARLRMERDIAKKSGGVLRAG
RAARYAWIEQMKNVYPIAVSCHVLQVSSSGYFRFRRSDGEALHLCSNELALAHIRAIHAEVKGEYGWPRIHKELQARGLRIGKERVRKLMAEHGIRAKTK
RKFVVTTDSKHSLPVAPDLVQRRFTPQAPNQLLCGDITYIQTDEGWLYLAAVIDMFSRQVVGWSLQPHMQSSLVKDAMAMAWWRRRPEPGAIFHSDRGSQ
YCSQSFQATLAGWGIRSSMSRKGNCWDNSPIESFWGRLKTACVHGQRFATREHARQAIMNWMAFYNYRRLHSSLGYLSPMQYEQRWYEAQRKKAA

 

Blast result :
Comments
ISPa91 is 85% aa similar to the transposase of ISCte2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPa91 is included in a Tn3 transposon (TnPa38).
References
1] Botelho J (2018) Direct submission to ISfinder