ISWci2
- Family IS3
- Group IS3
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP012873 | ND | Weissella cibaria | Weissella cibaria CH2 chromosome Weissella cibaria CH2 plasmid pMKC01 Weissella cibaria BM2 chromosome |
DNA section
IS Length : 1486 bp
Ends
IR Length : 23/33
IRL : tgctcttatgctagatttacggacagatttttctctgccctgtaaactaa
IRR : taggcgctgatgtcaagattatggacaggttttttcggcaccgagagggg
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
ATTGAGCGCTGATAATGTAC | TAACGTGGGCTAATTAAATG | 0 | |
GGCAGATAAGCTTCAAGAAG | ACGATCCACAAAATTACATT | 0 | |
TCAGGCTAAAACTGAAAATG | GGCAATGCTTGATGGTGCCA | 0 |
DNA sequence
tgctcttatgctagatttacggacagatttttctctgccctgtaaactaatagcagaggagcattttttatatgattcgatattcaccagaatttaagca
atctcttgtcgagatgcacaaccaagggcgttcttacactgaactagccgctgaatacgggccttcggccgattcaatccgtaactgggtcaaattgtac
acagtccacgaagtggacggcgaaaaatggacgcaagctgatgtaaacgcattacaaaaggaaaacgccaaacttcgcgaagaacttgagattttaaaac
gagccgcggtgttgctttcgaagtacaattaacgcaaaatcgcgagttaggactggaaatcattgaccggtcactcgcaatgggacaccgcataacaagc
gttttatcggctttgaactttgctcgaagcacgtattatcaatggaaaaactggcaacctagccaacgagaaacacggcgtaatgctctgaaaacggcaa
ttaaaggcgtttataacaccaatcgtggcatctatggctaccgtcgtatcgcagcgttcttgcgcttcattttgaagactgatgttggcgatcgtttggt
ttgggaactcatgaatgagctcaatctcaaatctcgcatggtcaagaagtcatacaagaagccaacaacaaccactgacacacctcaaaagcccaacctc
atgaagaagttggatgatttgagtggcgttctggcaactgacattacgtacatccagttgatgaatcgtgagtgggtttatctggcaaccgtctatgacc
cagaaaagcgtcgtgtagtctcatacggactaagcccagagatgaccaaggaattcgcaacatccgtcgtagttaaggctctaagagccaacggtaagcc
aaagatgattcacagcgacatgggaagccaatacacttctagcctgttcgaaggaacattacaaaactctggaataaatcactcttattcccgtaagggg
catccttacgataacgctcgtatcgagagcttccattcgctcatcaagcgcgaaatgatttatcacgaagagtacaggaccattgatgatgttcgtgtgt
ctgtcgagtggtatgtgaactggtacaacaacagtcgcattaactcacgcactgattggcttcaggccgcctagaccaatgtctggtttacttaatcacg
gttatcggcactagccggcacgacgccgtttataagccgtcacttatgaatttcaaaaattcgccttgtgaaggcttatttgttgcacgatacaacttgt
gaaatccggatggttcacaatctataccacctgttttctctcaccggcaggtctcggccctgttctacctacaaaaacaccgagaaatgacgccttttta
agctgaaatccgggttttgaaaaattttcggagtccggcccctctcggtgccgaaaaaacctgtccataatcttgacatcagcgcc
atctcttgtcgagatgcacaaccaagggcgttcttacactgaactagccgctgaatacgggccttcggccgattcaatccgtaactgggtcaaattgtac
acagtccacgaagtggacggcgaaaaatggacgcaagctgatgtaaacgcattacaaaaggaaaacgccaaacttcgcgaagaacttgagattttaaaac
gagccgcggtgttgctttcgaagtacaattaacgcaaaatcgcgagttaggactggaaatcattgaccggtcactcgcaatgggacaccgcataacaagc
gttttatcggctttgaactttgctcgaagcacgtattatcaatggaaaaactggcaacctagccaacgagaaacacggcgtaatgctctgaaaacggcaa
ttaaaggcgtttataacaccaatcgtggcatctatggctaccgtcgtatcgcagcgttcttgcgcttcattttgaagactgatgttggcgatcgtttggt
ttgggaactcatgaatgagctcaatctcaaatctcgcatggtcaagaagtcatacaagaagccaacaacaaccactgacacacctcaaaagcccaacctc
atgaagaagttggatgatttgagtggcgttctggcaactgacattacgtacatccagttgatgaatcgtgagtgggtttatctggcaaccgtctatgacc
cagaaaagcgtcgtgtagtctcatacggactaagcccagagatgaccaaggaattcgcaacatccgtcgtagttaaggctctaagagccaacggtaagcc
aaagatgattcacagcgacatgggaagccaatacacttctagcctgttcgaaggaacattacaaaactctggaataaatcactcttattcccgtaagggg
catccttacgataacgctcgtatcgagagcttccattcgctcatcaagcgcgaaatgatttatcacgaagagtacaggaccattgatgatgttcgtgtgt
ctgtcgagtggtatgtgaactggtacaacaacagtcgcattaactcacgcactgattggcttcaggccgcctagaccaatgtctggtttacttaatcacg
gttatcggcactagccggcacgacgccgtttataagccgtcacttatgaatttcaaaaattcgccttgtgaaggcttatttgttgcacgatacaacttgt
gaaatccggatggttcacaatctataccacctgttttctctcaccggcaggtctcggccctgttctacctacaaaaacaccgagaaatgacgccttttta
agctgaaatccgggttttgaaaaattttcggagtccggcccctctcggtgccgaaaaaacctgtccataatcttgacatcagcgcc
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
261 bp | 86 aa | 72 | 332 | + | No |
Description : First part of the transposase
ORF sequence :
MIRYSPEFKQSLVEMHNQGRSYTELAAEYGPSADSIRNWVKLYTVHEVDGEKWTQADVNALQKENAKLREELEILKRAAVLLSKYN
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
885 bp | 294 aa | 290 | 1174 | + | No |
Description : Second part of the transposase
ORF sequence :
DFKTSRGVAFEVQLTQNRELGLEIIDRSLAMGHRITSVLSALNFARSTYYQWKNWQPSQRETRRNALKTAIKGVYNTNRGIYGYRRIAAFLRFILKTDVG
DRLVWELMNELNLKSRMVKKSYKKPTTTTDTPQKPNLMKKLDDLSGVLATDITYIQLMNREWVYLATVYDPEKRRVVSYGLSPEMTKEFATSVVVKALRA
NGKPKMIHSDMGSQYTSSLFEGTLQNSGINHSYSRKGHPYDNARIESFHSLIKREMIYHEEYRTIDDVRVSVEWYVNWYNNSRINSRTDWLQAA
DRLVWELMNELNLKSRMVKKSYKKPTTTTDTPQKPNLMKKLDDLSGVLATDITYIQLMNREWVYLATVYDPEKRRVVSYGLSPEMTKEFATSVVVKALRA
NGKPKMIHSDMGSQYTSSLFEGTLQNSGINHSYSRKGHPYDNARIESFHSLIKREMIYHEEYRTIDDVRVSVEWYVNWYNNSRINSRTDWLQAA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1103 bp | 367 aa | 72 | 1174 | + | Yes |
Chemistry : DDE
ORF sequence :
MIRYSPEFKQSLVEMHNQGRSYTELAAEYGPSADSIRNWVKLYTVHEVDGEKWTQADVNALQKENAKLREELEILKTSRGVAFEVQLTQNRELGLEIIDR
SLAMGHRITSVLSALNFARSTYYQWKNWQPSQRETRRNALKTAIKGVYNTNRGIYGYRRIAAFLRFILKTDVGDRLVWELMNELNLKSRMVKKSYKKPTT
TTDTPQKPNLMKKLDDLSGVLATDITYIQLMNREWVYLATVYDPEKRRVVSYGLSPEMTKEFATSVVVKALRANGKPKMIHSDMGSQYTSSLFEGTLQNS
GINHSYSRKGHPYDNARIESFHSLIKREMIYHEEYRTIDDVRVSVEWYVNWYNNSRINSRTDWLQAA
SLAMGHRITSVLSALNFARSTYYQWKNWQPSQRETRRNALKTAIKGVYNTNRGIYGYRRIAAFLRFILKTDVGDRLVWELMNELNLKSRMVKKSYKKPTT
TTDTPQKPNLMKKLDDLSGVLATDITYIQLMNREWVYLATVYDPEKRRVVSYGLSPEMTKEFATSVVVKALRANGKPKMIHSDMGSQYTSSLFEGTLQNS
GINHSYSRKGHPYDNARIESFHSLIKREMIYHEEYRTIDDVRVSVEWYVNWYNNSRINSRTDWLQAA
Blast result :
Comments
ISWci2 is 66% aa similar to IS1520.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Korcari D. (2018) Direct submission to ISfinder
2] Fortina M.G., Ricci G., Quattrini M., Korcari D., paper in preparation
2] Fortina M.G., Ricci G., Quattrini M., Korcari D., paper in preparation