ISfinder Mirror Site

ISBcen27

Family ISNCY
Group IS1202

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CH482377	ND	Burkholderia cenocepacia	Burkholderia cenocepacia PC184

DNA section

IS Length : 1690 bp

Ends

IR Length : 33/50

IRL : TGTAGTAGCTAAGTTCAAATGTCCGCTTCTGGCTAAGTTCAGATGTCCGT
IRR : TGTCATACCCGGATTCAGATGTCCACCTTCCAGCTATTTAAAAATGTCCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATCGACATTT	TTGAT	GTAAGAGCAA	5

DNA sequence

TGTAGTAGCTAAGTTCAAATGTCCGCTTCTGGCTAAGTTCAGATGTCCGTTTTTCGACCCATCGTAAGCTGACCGGCTGCCGGTTGTCCGGCGCCGGAGG
CTGCGATGGCTGCAACGGAACGGATCACGATGACGATGCGCGAACTGGACAGGTTCAAGGTCATTCAGGACGTGGCGGACGGCAAGCTCAAGCCATGGCG
TGCGGCAGAACGGCTGGAGCTGACGACCCGGCAGGTTCGCCGGCTGGTCGCCAGGCTGCGGGAGCACGGGCCTCAAGGTCTCGTGTCGGGACGGTATGCG
AAGCCAAGCAACAACCGGCTGGACGCGGTAATTGTGGACCGGGCGCTGTCGATCATCCGCGATCGCTACGCCGATTTCGGGCCGACGTTGGCCTGCGAGA
AGCTCTGGGAGTGTCACGGCATTGGGCTGGCCAAGGAGACGGTCAGGAAGCTGATGACGGACGCTGGGCTCTGGATTCCGCGCCGGCAACGACCGCCGAA
GGTCTACCAGCCGCGAGCGCGTCGGGCGTGTCTGGGCGAACTGATCCAGATCGACGGCAGCGATCATCGGTGGTTCGAGGAACGGGCACCGGCCTGCACG
CTGCTGGTGTATGTGGACGATGCGACGAGCCGATTGATGATGCTGCACTTCACGCAGACCGAATCGACGTTCAGCTACTTCGAGGCCACGCGGACGTATA
TCGAACGCCATGGCAAGCCGGGAGCGTTCTACAGCGACAAGGCCAGCGTGTTCCGCAACGTGAAGCCGGGCAAGACTGGCAATCGCGTGACGCCGTTTGG
CCGGGCGATGTACGAGCTGAACATCGACACGTTCTGCGCGAACAGCAGCTCGGCCAAGGGGCGCGTCGAGCGCGCACACCTGACGTTGCAGGACCGATTG
GTCAAGGAGATGCGATTGCGCGGAATTAACACGGTGGCCGACGCCAACGCTTACGCGCCCTCCTTCATGGCCGCCTACAACACACGCTTCGCGAAGCCGC
CGAAGAGCGACTTCAATGCGCACCGGCCGCTGCGGGCCGACGAGAGTCTGGACTTGGCGCTGACGTGGCGCGAGCCGCGGAAAGTAACGAAGTCGCTGAC
AGTGCAGTATGACCGGGTGCTGTACTTGCTGGAGGACACGCCGGAGAATCGGAAGTTGATTGACCGGCAGATCGAGGTGTGGGAGTACCCGGATGGACGG
ATTGAACTACGGGCTGACGACCGGGTGTTGGACTACAGACAGTACGACCGCCTAACCGAAATCGATCAAGGCGCCGTCGTTGAGCACAAGCGCTTGAGTC
ATGTGCTGCAGGTCGCGCAAGCGCTTCAGGCGCAACGCGATAACAGCCGAATCGGTAAAGCGCCGTCACGGACACATCGCGGCGACTCAACCCGGACCAA
TCGAAACGACCCGCAAGCGGGAAAGAAGAAGCAACGCGAGATCACGCAGGCCGACGTCGAGCATGTGATCGTGGAGCTGGCGCAGCGTCGGCAAACCGCC
AAGCCAGCAGGCAAATCTGGCCGACGGTCTGCAAAAGCTGGAGAAACAAGCGTGAGCGCCCTGCCCGTTCAGGCGCCGACCTTCGACACTGCGTAACGGT
CAAGACGAGAAAGCAGAAGCAGCAAAACCAACCTAAAAACCGGACATTTTTAAATAGCTGGAAGGTGGACATCTGAATCCGGGTATGACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1491 bp	496 aa	106	1596	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAATERITMTMRELDRFKVIQDVADGKLKPWRAAERLELTTRQVRRLVARLREHGPQGLVSGRYAKPSNNRLDAVIVDRALSIIRDRYADFGPTLACEKL
WECHGIGLAKETVRKLMTDAGLWIPRRQRPPKVYQPRARRACLGELIQIDGSDHRWFEERAPACTLLVYVDDATSRLMMLHFTQTESTFSYFEATRTYIE
RHGKPGAFYSDKASVFRNVKPGKTGNRVTPFGRAMYELNIDTFCANSSSAKGRVERAHLTLQDRLVKEMRLRGINTVADANAYAPSFMAAYNTRFAKPPK
SDFNAHRPLRADESLDLALTWREPRKVTKSLTVQYDRVLYLLEDTPENRKLIDRQIEVWEYPDGRIELRADDRVLDYRQYDRLTEIDQGAVVEHKRLSHV
LQVAQALQAQRDNSRIGKAPSRTHRGDSTRTNRNDPQAGKKKQREITQADVEHVIVELAQRRQTAKPAGKSGRRSAKAGETSVSALPVQAPTFDTA

Blast result :

Comments

ISBcen27 is 70% aa similar to ISKpn21.

References

1] Isfinder submission (2018)
2] Birren,B., Lander,E., Galagan,J., Devon,K., Nusbaum,C., Borowsky,M.L., Jaffe,D., Butler,J., Alvarez,P., Gnerre,S., Grabherr,M., Kleber,M., Mauceli,E., Brockman,W., Young,S., LaButti,K., DeCaprio,D., Crawford,M., Koehrsen,M., Engels,R., Montgomery,P., Pearson,M., Howarth,C., White,J., Larson,L., Alvarado,L., Kodira,C., Zeng,Q., Yandava,C., Oleary,S., LiPuma,J. and Lory,S. (2005) Direct GenBank submission.