ISBt3
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF447491 | ND | Burkholderia thailandensis | Burkholderia thailandensis E125 Burkholderia virus phiE125 Bacteriophage phiE125 |
DNA section
IS Length : 1318 bp
Ends
IR Length : 22/27
IRL : TGAACCGCCCCGAGTTTGTGGAGGCTCCAACTCTGAGAGAATGGAGCCAT
IRR : TGAACCGCACCGGGAATCGTGGAGGCTGGTTGGTTTAAGTTAATGCGGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTCGCTGCT | GTC | CGCCTACCAA | 3 |
DNA sequence
TGAACCGCCCCGAGTTTGTGGAGGCTCCAACTCTGAGAGAATGGAGCCATGAACAGAAGCCAAGCAGTTTTCCCCGGAAGTCCGAGAGCGCGCAGTGCGC
CTGGTACGCGAGCAGCGTAGCGAGCACCCGTCGATGTGGGCGGCCGTCGAATCGATTGCGCCGATGATCGGCTGCACGCCGCAGACGCTGTTGGATTGGG
TTAAGCGCAACGAGGTCGACCGTGGAGAGCGCGATGGCGTGAGTACGGCCGAGCGTGAACGCATCAAAGCCTTGGAGCGCGAGGTCAAGGAACTGCGCCG
GACCAACGAGATTCTCAAACTGGCGAGCGCGTTTTTCGCTCAGGCGGAGCTCGACCGCCGTTTCAAGTCCTGAAGGCCTTCATTGATCAGCATCGCGACA
CCTTCGGGGTCGAGCCGATCTGCAAGGTCTTGCGGATTGCCCCGTCGGGCTACCGACGCCATGCAGCACAACTTCGCGATCCGTCGAAACGCTGCGCCCG
CGCGAAACGCGATGAGCTTTTGCAACCGGAGATCAAGCGTGTCTGGCAGGCCAACATGCAGGTCTACGGCGTGCCGAAGGTCTGGAAGCAGATGAACCGG
GAAGGCATTACGGTGGCACGCTGCACGGTCGGACGGTTGATGAAGCTGCAGGGCTTGCGTGGCGCAGTTCGCGGTAAGCGTGTTCGCACGACGATTACCG
ATGTGACCGCGCCGCGCCCGCTGGACCGAGTCAACCGGCAGTTCAAGGCTGACCGACCGAATCAGCTCTGGGTGTCGGATTTTACGTATGTCTCGACATG
GCAAGGCTGGCTGTACGTGGCATTCGTGATCGACGTGTTCGCCCGCCGTATTGTTGGCTGGCGGGTCAGCTCGTCGATGACCACGGACTTTGTTCTGGAT
GCACTTGAACAAGCGCTGTACGCCCGCCAACCGGGCGAGGACGGGACTTTGATTCATCATTCCGACAGGGGGTCTCAATACGTCAGCATTCGCTACAGCG
AACGGCTGGCTGAGGCCGGCATCGAGCCCTCAGTCGGCAGCCGGGGCGACAGCTACGACAATGCGCTGGCCGAAACGATCAACGGCCTGTACAAGACGGA
ACTGATTCATCGGCGCGCCCCTTGGAAAACGAGGGAATCCGTCGAACTGGCAACGCTGGAATGGGTCGCCTGGTACAACCATCATCGGCTGATGGAACCG
CTCGGCTATATCCCGCCCGCTGAAGCTGAGGCAAACTACTACAGACAACTCAGAAATGCCGCTGACGTGTCCGCATTAACTTAAACCAACCAGCCTCCAC
GATTCCCGGTGCGGTTCA
CTGGTACGCGAGCAGCGTAGCGAGCACCCGTCGATGTGGGCGGCCGTCGAATCGATTGCGCCGATGATCGGCTGCACGCCGCAGACGCTGTTGGATTGGG
TTAAGCGCAACGAGGTCGACCGTGGAGAGCGCGATGGCGTGAGTACGGCCGAGCGTGAACGCATCAAAGCCTTGGAGCGCGAGGTCAAGGAACTGCGCCG
GACCAACGAGATTCTCAAACTGGCGAGCGCGTTTTTCGCTCAGGCGGAGCTCGACCGCCGTTTCAAGTCCTGAAGGCCTTCATTGATCAGCATCGCGACA
CCTTCGGGGTCGAGCCGATCTGCAAGGTCTTGCGGATTGCCCCGTCGGGCTACCGACGCCATGCAGCACAACTTCGCGATCCGTCGAAACGCTGCGCCCG
CGCGAAACGCGATGAGCTTTTGCAACCGGAGATCAAGCGTGTCTGGCAGGCCAACATGCAGGTCTACGGCGTGCCGAAGGTCTGGAAGCAGATGAACCGG
GAAGGCATTACGGTGGCACGCTGCACGGTCGGACGGTTGATGAAGCTGCAGGGCTTGCGTGGCGCAGTTCGCGGTAAGCGTGTTCGCACGACGATTACCG
ATGTGACCGCGCCGCGCCCGCTGGACCGAGTCAACCGGCAGTTCAAGGCTGACCGACCGAATCAGCTCTGGGTGTCGGATTTTACGTATGTCTCGACATG
GCAAGGCTGGCTGTACGTGGCATTCGTGATCGACGTGTTCGCCCGCCGTATTGTTGGCTGGCGGGTCAGCTCGTCGATGACCACGGACTTTGTTCTGGAT
GCACTTGAACAAGCGCTGTACGCCCGCCAACCGGGCGAGGACGGGACTTTGATTCATCATTCCGACAGGGGGTCTCAATACGTCAGCATTCGCTACAGCG
AACGGCTGGCTGAGGCCGGCATCGAGCCCTCAGTCGGCAGCCGGGGCGACAGCTACGACAATGCGCTGGCCGAAACGATCAACGGCCTGTACAAGACGGA
ACTGATTCATCGGCGCGCCCCTTGGAAAACGAGGGAATCCGTCGAACTGGCAACGCTGGAATGGGTCGCCTGGTACAACCATCATCGGCTGATGGAACCG
CTCGGCTATATCCCGCCCGCTGAAGCTGAGGCAAACTACTACAGACAACTCAGAAATGCCGCTGACGTGTCCGCATTAACTTAAACCAACCAGCCTCCAC
GATTCCCGGTGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 339 bp | 92 aa | 35 | 373 | + | No |
Description : First part of the transposase
ORF sequence :
MRLVREQRSEHPSMWAAVESIAPMIGCTPQTLLDWVKRNEVDRGERDGVSTAERERIKALEREVKELRRTNEILKLASAFFAQAELDRRFKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1026 bp | 341 aa | 259 | 1284 | + | No |
Description : Second part of the transposase
ORF sequence :
THQSLGARGQGTAPDQRDSQTGERVFRSGGARPPFQVLKAFIDQHRDTFGVEPICKVLRIAPSGYRRHAAQLRDPSKRCARAKRDELLQPEIKRVWQANM
QVYGVPKVWKQMNREGITVARCTVGRLMKLQGLRGAVRGKRVRTTITDVTAPRPLDRVNRQFKADRPNQLWVSDFTYVSTWQGWLYVAFVIDVFARRIVG
WRVSSSMTTDFVLDALEQALYARQPGEDGTLIHHSDRGSQYVSIRYSERLAEAGIEPSVGSRGDSYDNALAETINGLYKTELIHRRAPWKTRESVELATL
EWVAWYNHHRLMEPLGYIPPAEAEANYYRQLRNAADVSALT
QVYGVPKVWKQMNREGITVARCTVGRLMKLQGLRGAVRGKRVRTTITDVTAPRPLDRVNRQFKADRPNQLWVSDFTYVSTWQGWLYVAFVIDVFARRIVG
WRVSSSMTTDFVLDALEQALYARQPGEDGTLIHHSDRGSQYVSIRYSERLAEAGIEPSVGSRGDSYDNALAETINGLYKTELIHRRAPWKTRESVELATL
EWVAWYNHHRLMEPLGYIPPAEAEANYYRQLRNAADVSALT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1250 bp | 396 aa | 35 | 1284 | + | Yes |
Chemistry : DDE
ORF sequence :
MRLVREQRSEHPSMWAAVESIAPMIGCTPQTLLDWVKRNEVDRGERDGVSTAERERIKALEREVKELRRTNEILKLASAFFRSGGARPPFQVLKAFIDQH
RDTFGVEPICKVLRIAPSGYRRHAAQLRDPSKRCARAKRDELLQPEIKRVWQANMQVYGVPKVWKQMNREGITVARCTVGRLMKLQGLRGAVRGKRVRTT
ITDVTAPRPLDRVNRQFKADRPNQLWVSDFTYVSTWQGWLYVAFVIDVFARRIVGWRVSSSMTTDFVLDALEQALYARQPGEDGTLIHHSDRGSQYVSIR
YSERLAEAGIEPSVGSRGDSYDNALAETINGLYKTELIHRRAPWKTRESVELATLEWVAWYNHHRLMEPLGYIPPAEAEANYYRQLRNAADVSALT
RDTFGVEPICKVLRIAPSGYRRHAAQLRDPSKRCARAKRDELLQPEIKRVWQANMQVYGVPKVWKQMNREGITVARCTVGRLMKLQGLRGAVRGKRVRTT
ITDVTAPRPLDRVNRQFKADRPNQLWVSDFTYVSTWQGWLYVAFVIDVFARRIVGWRVSSSMTTDFVLDALEQALYARQPGEDGTLIHHSDRGSQYVSIR
YSERLAEAGIEPSVGSRGDSYDNALAETINGLYKTELIHRRAPWKTRESVELATLEWVAWYNHHRLMEPLGYIPPAEAEANYYRQLRNAADVSALT
Blast result :
Comments
ISBt3 is 97% aa similar to IS1416.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2018)
2] Woods,D.E., Jeddeloh,J.A., Fritz,D.L. and DeShazer,D. (2002) J. Bacteriol. 184 (14), 4003-4017.
2] Woods,D.E., Jeddeloh,J.A., Fritz,D.L. and DeShazer,D. (2002) J. Bacteriol. 184 (14), 4003-4017.