ISPye57
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP024422 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 Paracoccus yeei TT13 |
DNA section
IS Length : 1271 bp
Ends
IR Length : 32/44
IRL : TGACCTGCCACGGGATTTTTCCTCCATCTACGATTAGAGTCCGGCCTATG
IRR : TGACCTGCCCCTGAGCTTTCATCCAGCCGCGATCGGAGCCGGTAGCTGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tcttgtgcaa | GCCA | tcatcgagga | 4 |
| gcaggccaga | aaagctcgga | 0 | |
| atcaggttgt | CAGG | ctctctgttg | 4 |
| ggggctaagg | ctgaaggccc | 0 | |
| gtggcgtgat | atcgctgcaa | 0 | |
| tttcgtgtcg | aagccggtca | 0 |
DNA sequence
TGACCTGCCACGGGATTTTTCCTCCATCTACGATTAGAGTCCGGCCTATGGAGAGGACGGACAATGAAGAAGGCACGACTGACGGAAGAGCAGATCATTG
GCATCCTGCAGGAGCATGATGCCGGTGCGAAGTGCGCCGATCTCTGCCGCAAGCACGGGATGTCGGAAGGCACGTTCTATGCCTGGAAGGCGAAGTATTC
CGGGATGACGGTGTCCGAGGCCAAGCGGCTGAAGGCGCTGGAGGACGAGAACGCCAAGCTGAAGCGGCTCTTGGCCGAGCAGATGCTCGACATGGCCGCG
ATGAAGGAACTGCTGTCAAAAAAATGGTGACGCCCGCCGTGAAGCGCGAAGCTGTCGCGCATCTGAAGGCCCATCTCGGGCTGTCGGAACGGCGGGCGTG
CCAGATTGCCGGAGCGGACCGCAAGATGGTGCGCTACCAGTCGCAGCGCGCACCGGACACGGCGCTGCGCGGTCGGTTGCGGGATCTGGCCAACGAGCGG
CGCCGGTTCGGTTACCGGCGGCTGTTCGTCCTGCTCAGGCGCGAAGGCGAGTCGTCGGGGATCAACCGCATCTACCGTCTCTACCGAGAAGAAGGGTTAA
CGGTGCGTAAACGCAAAGCACGCCGCAAGGCCATCGGCACAAGGGCCCCGATCCTGATGGAGGCGCGGGCCAATGCGCGCTGGTCGCTCGACTTCGTGCA
TGACCAGTTCGCCTGCGGGCGGCGCTTCCGGGTGCTGAACGTGGTGGACGATGTGACGCGCGAGTGCCTTGCAGCGATCCCCGACACCTCGATCTCAGGC
CGCCGTGTGGCGCGGGAGCTGACGGCCTTGATCGAACGCCGCGGGAAGCCCGGGATGATCGTGTCTGACAACGGGACCGAACTGACCTCGAACGCGATCC
TGAAGTGGTGCGCCGAGCACAAGGTCGAATGGCACTACATCGCGCCAGGCAAGCCGATGCAGAATGGTTTCGTGGAAAGCTTCAACGGCAGGATGCGGGA
CGAATTTCTGAACGAGACCCTGTTCCGCAACCTCGCCCATGCCCGCGATCTGATCGCAGCTTGGGTCACCGACTACAACACCGAAAGGCCCCATTCTGCC
CTCGGCTACCAGACCCCCGCAGGCTTCGCCCTGCACCTGACCACCGCAATCGCCCGACCCGCTGCACGCGATGAGAGTTCCGCGCGCCGGGCGATTGCTC
AACCCACGCCGAAAGGCGTAAATCAGCTACCGGCTCCGATCGCGGCTGGATGAAAGCTCAGGGGCAGGTCA
GCATCCTGCAGGAGCATGATGCCGGTGCGAAGTGCGCCGATCTCTGCCGCAAGCACGGGATGTCGGAAGGCACGTTCTATGCCTGGAAGGCGAAGTATTC
CGGGATGACGGTGTCCGAGGCCAAGCGGCTGAAGGCGCTGGAGGACGAGAACGCCAAGCTGAAGCGGCTCTTGGCCGAGCAGATGCTCGACATGGCCGCG
ATGAAGGAACTGCTGTCAAAAAAATGGTGACGCCCGCCGTGAAGCGCGAAGCTGTCGCGCATCTGAAGGCCCATCTCGGGCTGTCGGAACGGCGGGCGTG
CCAGATTGCCGGAGCGGACCGCAAGATGGTGCGCTACCAGTCGCAGCGCGCACCGGACACGGCGCTGCGCGGTCGGTTGCGGGATCTGGCCAACGAGCGG
CGCCGGTTCGGTTACCGGCGGCTGTTCGTCCTGCTCAGGCGCGAAGGCGAGTCGTCGGGGATCAACCGCATCTACCGTCTCTACCGAGAAGAAGGGTTAA
CGGTGCGTAAACGCAAAGCACGCCGCAAGGCCATCGGCACAAGGGCCCCGATCCTGATGGAGGCGCGGGCCAATGCGCGCTGGTCGCTCGACTTCGTGCA
TGACCAGTTCGCCTGCGGGCGGCGCTTCCGGGTGCTGAACGTGGTGGACGATGTGACGCGCGAGTGCCTTGCAGCGATCCCCGACACCTCGATCTCAGGC
CGCCGTGTGGCGCGGGAGCTGACGGCCTTGATCGAACGCCGCGGGAAGCCCGGGATGATCGTGTCTGACAACGGGACCGAACTGACCTCGAACGCGATCC
TGAAGTGGTGCGCCGAGCACAAGGTCGAATGGCACTACATCGCGCCAGGCAAGCCGATGCAGAATGGTTTCGTGGAAAGCTTCAACGGCAGGATGCGGGA
CGAATTTCTGAACGAGACCCTGTTCCGCAACCTCGCCCATGCCCGCGATCTGATCGCAGCTTGGGTCACCGACTACAACACCGAAAGGCCCCATTCTGCC
CTCGGCTACCAGACCCCCGCAGGCTTCGCCCTGCACCTGACCACCGCAATCGCCCGACCCGCTGCACGCGATGAGAGTTCCGCGCGCCGGGCGATTGCTC
AACCCACGCCGAAAGGCGTAAATCAGCTACCGGCTCCGATCGCGGCTGGATGAAAGCTCAGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 88 aa | 64 | 327 | + | No |
Description : First part of the transposase
ORF sequence :
MKKARLTEEQIIGILQEHDAGAKCADLCRKHGMSEGTFYAWKAKYSGMTVSEAKRLKALEDENAKLKRLLAEQMLDMAAMKELLSKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1134 bp | 377 aa | 120 | 1253 | + | No |
Description : Second part of the transposase
ORF sequence :
CRCEVRRSLPQARDVGRHVLCLEGEVFRDDGVRGQAAEGAGGRERQAEAALGRADARHGRDEGTAVKKMVTPAVKREAVAHLKAHLGLSERRACQIAGAD
RKMVRYQSQRAPDTALRGRLRDLANERRRFGYRRLFVLLRREGESSGINRIYRLYREEGLTVRKRKARRKAIGTRAPILMEARANARWSLDFVHDQFACG
RRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGKPGMIVSDNGTELTSNAILKWCAEHKVEWHYIAPGKPMQNGFVESFNGRMRDEFLNET
LFRNLAHARDLIAAWVTDYNTERPHSALGYQTPAGFALHLTTAIARPAARDESSARRAIAQPTPKGVNQLPAPIAAG
RKMVRYQSQRAPDTALRGRLRDLANERRRFGYRRLFVLLRREGESSGINRIYRLYREEGLTVRKRKARRKAIGTRAPILMEARANARWSLDFVHDQFACG
RRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGKPGMIVSDNGTELTSNAILKWCAEHKVEWHYIAPGKPMQNGFVESFNGRMRDEFLNET
LFRNLAHARDLIAAWVTDYNTERPHSALGYQTPAGFALHLTTAIARPAARDESSARRAIAQPTPKGVNQLPAPIAAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 396 aa | 64 | 1253 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKARLTEEQIIGILQEHDAGAKCADLCRKHGMSEGTFYAWKAKYSGMTVSEAKRLKALEDENAKLKRLLAEQMLDMAAMKELLSKKLVTPAVKREAVAH
LKAHLGLSERRACQIAGADRKMVRYQSQRAPDTALRGRLRDLANERRRFGYRRLFVLLRREGESSGINRIYRLYREEGLTVRKRKARRKAIGTRAPILME
ARANARWSLDFVHDQFACGRRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGKPGMIVSDNGTELTSNAILKWCAEHKVEWHYIAPGKPMQ
NGFVESFNGRMRDEFLNETLFRNLAHARDLIAAWVTDYNTERPHSALGYQTPAGFALHLTTAIARPAARDESSARRAIAQPTPKGVNQLPAPIAAG
LKAHLGLSERRACQIAGADRKMVRYQSQRAPDTALRGRLRDLANERRRFGYRRLFVLLRREGESSGINRIYRLYREEGLTVRKRKARRKAIGTRAPILME
ARANARWSLDFVHDQFACGRRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGKPGMIVSDNGTELTSNAILKWCAEHKVEWHYIAPGKPMQ
NGFVESFNGRMRDEFLNETLFRNLAHARDLIAAWVTDYNTERPHSALGYQTPAGFALHLTTAIARPAARDESSARRAIAQPTPKGVNQLPAPIAAG
Blast result :
Comments
ISPye57 is 93% aa similar to ISOba1.
ISPye57 was identified by in silico sequence analysis of Paracoccus yeei strains TT13 (6 copies present in genome) and FDAARGOS_252 (1 isoform, reocnstructed). The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPye57 was identified by in silico sequence analysis of Paracoccus yeei strains TT13 (6 copies present in genome) and FDAARGOS_252 (1 isoform, reocnstructed). The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References