ISPye52

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Paracoccus yeei
Paracoccus yeei
Paracoccus yeei CCUG 32053
DNA section
IS Length : 1323 bp

Ends


IR Length : 27

IRL : TGAAGCGCCCCGGGTTTACCGGAGAGTAAAACACTCGAAGGATGAGCCCC
IRR : TGAAGCGCCCCGGGTTTACCGGAGAGTTTTTTGTTCAAAACTTACGCGGC

Insertion site


Left flankDirect repeatRight flankDR Length
atcacgggaccctggagctt0

DNA sequence

TGAAGCGCCCCGGGTTTACCGGAGAGTAAAACACTCGAAGGATGAGCCCCATGAGCCAGAAGAGATATACCCCCGCCTATCCTGCCGAACTTCGCGATCG
CGGCGTTCGGCTTTTCCGCGACCATCGTGGCGAATATGCCAGTGACAATGCTGCTTACCGGGCGATTGCGCCGAAGCTGGGCTGCTCTCCGGACAGTCTT
CGCGTGTGGTGCCAGCAGGCCGAACGTGACGCGGGACAGCGGGCCGGACTGACAAGCGCAGAGAAGGATCGGATCAAGGAATTGGAGCGCGAGGTCCGTG
AACTTCGGACGGCCAACGAGATTCTGAAGAAGGCGAGCGCGTATTTCGCGCAGGCGGAGCTCGACCGCCCGTTCCGGAAATGATCGCGTTCATTGCAGAT
CATCGAGAGGTCTTCGGGGTCGAGCCGATCTGTCGCGTGCTGCCGATCGCCCCCTCGACATACTACGCGCGCAGGGCGATCCTCCGCGACCCCGATCGAG
CCTCGGATCGGGCGAAGCGGGATGCACGGAACAGCGCGGAGATCAAGCGTGCTTTCGATGCCAGCCGCGGCCGCTACGGGGCCCGCAAGATCTGGCATCA
ACTGCGCCGCGAGGGAAAGGACATCGCCCGCTGCACGGTCGAACGGCTCATGAAAGCTCTCGATTTGCAGGGCGTGGTGCGCGGCAAGAAGCGCACGACC
CTTCCTGACCCGGCCCAGGCCTGCCCGGACGACAAGGTGAACCGGGAATTTACAGCCGCGACCCCGAACCAGCTTTGGGTCAGCGACTTCACCTATGTCT
CCAGCTGGGCCGGCATGGTCTATGTCGCCTTCGTCATCGATGTCTTCGCCCGCAGGATTGTCGGCTGGCGGGTGTCGACCTCCATGACCACCAGCTTCGT
GCTCGATGCACTGAACCAGGCGATCTGTCAGCGTTGTTCTGATAAAAGCGATGCGCTGATCCACCATTCGGATCGCGGCAGCCAATACCTGTCGATCCGC
TACACCGAGCGGCTGGAGGAAGCCGGGATCGATCCTTCGGTGGGCAGCGTCGGTGACAGCTATGATAACGCCCTGGCCGAAAGCACCATCGGATTGTTCA
AGACCGAGGTCATCAACTTTCTCAGCCCGTGGAAATCCGTCGGCCAGGTCGAATGGGAAACCCTCAAATGGGTCAGCTGGTATAACTCAGAGCGGCTGCA
CAGCGCCATCGGCTATGTCACGCCGCAAGAAGCGGAGGAGAAGTTCTATGCGAATGTGAACACCCTTGAAAAAGCCGCGTAAGTTTTGAACAAAAAACTC
TCCGGTAAACCCGGGGCGCTTCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
333 bp110 aa51383+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSQKRYTPAYPAELRDRGVRLFRDHRGEYASDNAAYRAIAPKLGCSPDSLRVWCQQAERDAGQRAGLTSAEKDRIKELEREVRELRTANEILKKASAYFA
QAELDRPFRK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
981 bp326 aa3021282+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TSDGQRDSEEGERVFRAGGARPPVPEMIAFIADHREVFGVEPICRVLPIAPSTYYARRAILRDPDRASDRAKRDARNSAEIKRAFDASRGRYGARKIWHQ
LRREGKDIARCTVERLMKALDLQGVVRGKKRTTLPDPAQACPDDKVNREFTAATPNQLWVSDFTYVSSWAGMVYVAFVIDVFARRIVGWRVSTSMTTSFV
LDALNQAICQRCSDKSDALIHHSDRGSQYLSIRYTERLEEAGIDPSVGSVGDSYDNALAESTIGLFKTEVINFLSPWKSVGQVEWETLKWVSWYNSERLH
SAIGYVTPQEAEEKFYANVNTLEKAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1232 bp410 aa511282+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQKRYTPAYPAELRDRGVRLFRDHRGEYASDNAAYRAIAPKLGCSPDSLRVWCQQAERDAGQRAGLTSAEKDRIKELEREVRELRTANEILKKASAYFR
AGGARPPVPEMIAFIADHREVFGVEPICRVLPIAPSTYYARRAILRDPDRASDRAKRDARNSAEIKRAFDASRGRYGARKIWHQLRREGKDIARCTVERL
MKALDLQGVVRGKKRTTLPDPAQACPDDKVNREFTAATPNQLWVSDFTYVSSWAGMVYVAFVIDVFARRIVGWRVSTSMTTSFVLDALNQAICQRCSDKS
DALIHHSDRGSQYLSIRYTERLEEAGIDPSVGSVGDSYDNALAESTIGLFKTEVINFLSPWKSVGQVEWETLKWVSWYNSERLHSAIGYVTPQEAEEKFY
ANVNTLEKAA

 

Blast result :
Comments
ISPye52 is 75% aa similar to ISMex33.
ISPye52 was identified by in silico sequence analysis of Paracoccus yeei CCUG 32053 (1 copy). The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.