ISPa195
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MF770250 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa |
DNA section
IS Length : 1190 bp
Ends
IR Length : 34/41
IRL : GGGTAATCCCCCCGTAAAACCAGCGTCTTGAGAAGTAGAGACCTCGCAAG
IRR : GGGTAATCCCCCCTTAAACCATTGCTCTGAGAAGTAGAGCTATGCGGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTTCGCCCCACTTCAGCAT | TGTCTTCGATGAACCCCTTC | 0 |
DNA sequence
GGGTAATCCCCCCGTAAAACCAGCGTCTTGAGAAGTAGAGACCTCGCAAGGAGAATCTCTACTATGAAGAAGTCACGTTTTACAGACAGCCAAATCATCG
ATGCGCTCAAGCGTGCAGAGGCTGGCTTGGCTGTGCCCGAGCTGTGCCGTGAACTTGGCATCAGTGCAGCTACGTTCTACAAGTGGCGTGCCAAGTTCGG
CGGAATGGATGCCTCGTTGATGGCTCGTATGAAGGAGCTCGAAGAAGAGAACCGCCGGCTGCGCAAGATGTACATCGAAGAAAAGCTCAAGGCAGAGGTG
GTGGCGGAGGCGCTTGCAAAAAAGTGGTGAAGCCATCTCGCAGACGCGAGATGGCAGAACTTGCAGTGATACGCCGAGGGATGAGCATTCGCTTGGCTTG
CCAAGCGTTCCAAATTAGTCAGGCCTGCTACAGGTACAAGGCACAGCGCTGTATCGAGGACGATGAGATTGCGCAGTGGCTGCTGCGCCTGACGGATAAC
AACCGCAATTGGGGCTTTGGTTTGTGCTTCCTGTATTTGCGCAACGTCAGAGGCTTCAAATGGAACCACAAGCGGGTTTACCGGATTTACCGGGAGTTGG
AGCTCAATTTGCGCATTCGGCCACGCAAGCGCTTGGAGCGTGCCAAACCTGAGCCACTGACAGTGCCAGCAAACCCAGATGTTGTTTGGTCAATGGACTT
TATGCATGACCAGCTTGAAGATGGCCGCAGTATCCGGCTGCTCAACGTCATTGATGACTTCAACCGTGAAGCACTGGGTATTGAGGTGGACTTCTCATTG
CCCGCGCAGCGTGTGATTCGGGTCTTGGAGCAAATCATGCAGTGGCGTGGCAAGCCGCAAGTGATTCGCTGTGACAACGGGCCCGAATACGTCAGCGCAA
CCCTGCAAAACTGGGCGAGCGCCAAAGGTATCCGTTTGGAATACATCCAGCCAGGCAAGCCTCAGCAAAATGCCTATGTGGAGCGCTTCAACAGGACCGT
TCGCTATGAATGGCTGTCGCAGTACCATTGGCGCAATCTTGATGAAGTTCGAGATTACGCCACGCAATGGATGTGGCGGTACAACCATGAACGCCCAAAT
ATGGCATTGGGCGGCATCACCCCCAAACAGCGGCTTGCCATGGCCGCATAGCTCTACTTCTCAGAGCAATGGTTTAAGGGGGGATTACCC
ATGCGCTCAAGCGTGCAGAGGCTGGCTTGGCTGTGCCCGAGCTGTGCCGTGAACTTGGCATCAGTGCAGCTACGTTCTACAAGTGGCGTGCCAAGTTCGG
CGGAATGGATGCCTCGTTGATGGCTCGTATGAAGGAGCTCGAAGAAGAGAACCGCCGGCTGCGCAAGATGTACATCGAAGAAAAGCTCAAGGCAGAGGTG
GTGGCGGAGGCGCTTGCAAAAAAGTGGTGAAGCCATCTCGCAGACGCGAGATGGCAGAACTTGCAGTGATACGCCGAGGGATGAGCATTCGCTTGGCTTG
CCAAGCGTTCCAAATTAGTCAGGCCTGCTACAGGTACAAGGCACAGCGCTGTATCGAGGACGATGAGATTGCGCAGTGGCTGCTGCGCCTGACGGATAAC
AACCGCAATTGGGGCTTTGGTTTGTGCTTCCTGTATTTGCGCAACGTCAGAGGCTTCAAATGGAACCACAAGCGGGTTTACCGGATTTACCGGGAGTTGG
AGCTCAATTTGCGCATTCGGCCACGCAAGCGCTTGGAGCGTGCCAAACCTGAGCCACTGACAGTGCCAGCAAACCCAGATGTTGTTTGGTCAATGGACTT
TATGCATGACCAGCTTGAAGATGGCCGCAGTATCCGGCTGCTCAACGTCATTGATGACTTCAACCGTGAAGCACTGGGTATTGAGGTGGACTTCTCATTG
CCCGCGCAGCGTGTGATTCGGGTCTTGGAGCAAATCATGCAGTGGCGTGGCAAGCCGCAAGTGATTCGCTGTGACAACGGGCCCGAATACGTCAGCGCAA
CCCTGCAAAACTGGGCGAGCGCCAAAGGTATCCGTTTGGAATACATCCAGCCAGGCAAGCCTCAGCAAAATGCCTATGTGGAGCGCTTCAACAGGACCGT
TCGCTATGAATGGCTGTCGCAGTACCATTGGCGCAATCTTGATGAAGTTCGAGATTACGCCACGCAATGGATGTGGCGGTACAACCATGAACGCCCAAAT
ATGGCATTGGGCGGCATCACCCCCAAACAGCGGCTTGCCATGGCCGCATAGCTCTACTTCTCAGAGCAATGGTTTAAGGGGGGATTACCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
AAAAAAG
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 64 | 330 | + | No |
Description : First part of the transposase
ORF sequence :
MKKSRFTDSQIIDALKRAEAGLAVPELCRELGISAATFYKWRAKFGGMDASLMARMKELEEENRRLRKMYIEEKLKAEVVAEALAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 999 bp | 332 aa | 153 | 1151 | + | No |
Description : Second part of the transposase
ORF sequence :
TWHQCSYVLQVACQVRRNGCLVDGSYEGARRREPPAAQDVHRRKAQGRGGGGGACKKVVKPSRRREMAELAVIRRGMSIRLACQAFQISQACYRYKAQRC
IEDDEIAQWLLRLTDNNRNWGFGLCFLYLRNVRGFKWNHKRVYRIYRELELNLRIRPRKRLERAKPEPLTVPANPDVVWSMDFMHDQLEDGRSIRLLNVI
DDFNREALGIEVDFSLPAQRVIRVLEQIMQWRGKPQVIRCDNGPEYVSATLQNWASAKGIRLEYIQPGKPQQNAYVERFNRTVRYEWLSQYHWRNLDEVR
DYATQWMWRYNHERPNMALGGITPKQRLAMAA
IEDDEIAQWLLRLTDNNRNWGFGLCFLYLRNVRGFKWNHKRVYRIYRELELNLRIRPRKRLERAKPEPLTVPANPDVVWSMDFMHDQLEDGRSIRLLNVI
DDFNREALGIEVDFSLPAQRVIRVLEQIMQWRGKPQVIRCDNGPEYVSATLQNWASAKGIRLEYIQPGKPQQNAYVERFNRTVRYEWLSQYHWRNLDEVR
DYATQWMWRYNHERPNMALGGITPKQRLAMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 64 | 1151 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKSRFTDSQIIDALKRAEAGLAVPELCRELGISAATFYKWRAKFGGMDASLMARMKELEEENRRLRKMYIEEKLKAEVVAEALAKKVVKPSRRREMAEL
AVIRRGMSIRLACQAFQISQACYRYKAQRCIEDDEIAQWLLRLTDNNRNWGFGLCFLYLRNVRGFKWNHKRVYRIYRELELNLRIRPRKRLERAKPEPLT
VPANPDVVWSMDFMHDQLEDGRSIRLLNVIDDFNREALGIEVDFSLPAQRVIRVLEQIMQWRGKPQVIRCDNGPEYVSATLQNWASAKGIRLEYIQPGKP
QQNAYVERFNRTVRYEWLSQYHWRNLDEVRDYATQWMWRYNHERPNMALGGITPKQRLAMAA
AVIRRGMSIRLACQAFQISQACYRYKAQRCIEDDEIAQWLLRLTDNNRNWGFGLCFLYLRNVRGFKWNHKRVYRIYRELELNLRIRPRKRLERAKPEPLT
VPANPDVVWSMDFMHDQLEDGRSIRLLNVIDDFNREALGIEVDFSLPAQRVIRVLEQIMQWRGKPQVIRCDNGPEYVSATLQNWASAKGIRLEYIQPGKP
QQNAYVERFNRTVRYEWLSQYHWRNLDEVRDYATQWMWRYNHERPNMALGGITPKQRLAMAA
Blast result :
Comments
ISPa195 is 84% aa similar to ISAs22.
The sequence was found in the oprD gene of Pseudomonas aeruginosa at position 195.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The sequence was found in the oprD gene of Pseudomonas aeruginosa at position 195.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Yuliya Bocharova (2018) Direct submission.