ISPye51

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Paracoccus yeei
Paracoccus yeei
Paracoccus yeei CCUG 32053
DNA section
IS Length : 841 bp

Ends


IR Length : 9/15

IRL : GGCTCCAGACCCATTGATTTCAATCTGTTAGCGTGATTTAGGCTCTGCAA
IRR : GCGTCAGGACTCGTTCTGGATCATAGCCAGAACAAGACGGTTGCCGCGAG

Comments : Ends based on alignment with other IS from this family.

Insertion site


Left flankDirect repeatRight flankDR Length
ggcccgctccTAtgtacgggag2

DNA sequence

GGCTCCAGACCCATTGATTTCAATCTGTTAGCGTGATTTAGGCTCTGCAAGGAGACCTGATCCATGAGCAATCTTTACTGGCTGACCGACGCCCAGATGG
AGCGCCTCAAGCCGTTCTTTCCTAAGAGCCATGGCAAGCCCCGCGTCGATGACCGTCGCGTTCTGAGCGGTATAATCTTCATCAATCGCAATGGGTTGCG
CTGGTGTGACGCGCCGAAGGAATACGGCCCGGCCAAGACCCTCTACAACCGTTGGAAGCGCTGGAGCGACAATGGCGTCTTCGCCCGGATCATGGTGGGC
CTTGCCGCGGAGAGCGCCGAGCACAAGACGATCATGATCGATGCAACCTACCTCAAGGCATACCGGACGGCATCAAGCCTGTGCGTGAAAAAGGGGGGCG
TGGGCGCCAGATCGGGCGAACCAAAGGCGGCATGAACACCAAGCTGCACGCCGTTGCCGATGCGAAGGGGCGGCCGATCGGGTTTTTCATGTCGGCCGGT
CAGGTCAGCGATTACACCGGCGCAGCGGCGCTGCTGGGCAGCCTTCCGAAGGCGGGCTGGCTTCTGGCCGATCGGGGCTATGACGCCGACTGGTTCAGAG
AAGCATTGAAAGACATCGGGATAAAGGTTTGCATCCCGGGCAGGAAGTCCCGCAAGAAGGCCGTCAAATACGACAAGCGGCGTTACAAACAACGCAACCG
CATCGAGATCATGTTCGGGCGGCTGAAGGACTGGAGGCGTGTCGCGACGCGTTACGACAGATGCCCACAGACGTTCTTCTCTGCCATCTTGCTCGCGGCA
ACCGTCTTGTTCTGGCTATGATCCAGAACGAGTCCTGACGC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
372 bp123 aa64435+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSNLYWLTDAQMERLKPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIMVGLAAESAEHKTIMIDATYLKAY
RTASSLCVKKGGVGARSGEPKAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
612 bp203 aa210821+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RAEGIRPGQDPLQPLEALERQWRLRPDHGGPCRGERRAQDDHDRCNLPQGIPDGIKPVREKGGRGRQIGRTKGGMNTKLHAVADAKGRPIGFFMSAGQVS
DYTGAAALLGSLPKAGWLLADRGYDADWFREALKDIGIKVCIPGRKSRKKAVKYDKRRYKQRNRIEIMFGRLKDWRRVATRYDRCPQTFFSAILLAATVL
FWL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
758 bp252 aa64821+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSNLYWLTDAQMERLKPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIMVGLAAESAEHKTIMIDATYLKAY
RTASSLCVKKGGRGRQIGRTKGGMNTKLHAVADAKGRPIGFFMSAGQVSDYTGAAALLGSLPKAGWLLADRGYDADWFREALKDIGIKVCIPGRKSRKKA
VKYDKRRYKQRNRIEIMFGRLKDWRRVATRYDRCPQTFFSAILLAATVLFWL

 

Blast result :
Comments
ISPye51 is 98% aa similar to ISPaes2.
ISPye51 was identified by in silico sequence analysis of Paracoccus yeei CCUG 32053 (1 copy).The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.