ISPye49
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP025430 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei CCUG 32053 Paracoccus zhejiangensis strain J6 |
DNA section
IS Length : 841 bp
Ends
IR Length : 21/23
IRL : GGCTCCAGACCCATTGATTTCAGGCGTTTGACATGATTCAGGCTCCGCAA
IRR : GGGTCCAGACTCATTGAATTTCACAGCCAGAACAAGACGGTTGCGGCGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| agggattgtc | TA | agagagctcc | 2 |
DNA sequence
GGCTCCAGACCCATTGATTTCAGGCGTTTGACATGATTCAGGCTCCGCAAGGAGACCTGATCGATGAGCAATCTTTACTGGCTGAGCGATGCCCAGATGG
AACGCCTGAAGCCGTTCTTTCCCAAGAGTCATGGCAAGCCCCGCGTCGATGATCGCCGCGTTCTCAGCCGCATAATATTCATCAACTGCAATGGGTTGCG
GTGGTGTGATGCGCCGAAGGAATATGGGCCCGCCAAGACCCTCTACAACCGTTGGAAGCGCTGGAGTGACAACGGAGTCTTCGCCAGGATCATGGTCGGC
CTGGCCGCTGAGAGCGCCGATCACAAGACGATCATGATCGATGCGACCTATCTCAAGGCACACCGCACGGCGTCGAGCCTGTGCGTGAAAAAGGGGGGCG
CGCACGCCAGATCGGGCGGACAAAAGGCGGCATGAACACCAAACTGCACGCCGTTGCCGATGCCACGGGGCGGCCGATCGGGTTCTTCATGTCGGCCGGC
GAGGTCAGCGACTACACAGGCGCCGCGGCTCTTCTGGGCAGTCTGCCGAAGGCGGGTTGGCTGCTGGCTGACAGGGGCTACGACGCTGATTGGTTCAGAG
AAGCGTTGAAAGACAGCGGGATAAAGGTCTGCATCCCCGGTCGAAAGTCCCGCAGGAAGGCCGTCAAATACGACAAGCGGCGCTACAAACGGCGCAACCG
CATCGAGATCATGTTCGGCCGTCTGAAGGATTGGCGGCGCGTCGCCACCCGATACGACCGCTGCCCGGAAACCTTCTTCTCCGCGATCATGCTCGCCGCA
ACCGTCTTGTTCTGGCTGTGAAATTCAATGAGTCTGGACCC
AACGCCTGAAGCCGTTCTTTCCCAAGAGTCATGGCAAGCCCCGCGTCGATGATCGCCGCGTTCTCAGCCGCATAATATTCATCAACTGCAATGGGTTGCG
GTGGTGTGATGCGCCGAAGGAATATGGGCCCGCCAAGACCCTCTACAACCGTTGGAAGCGCTGGAGTGACAACGGAGTCTTCGCCAGGATCATGGTCGGC
CTGGCCGCTGAGAGCGCCGATCACAAGACGATCATGATCGATGCGACCTATCTCAAGGCACACCGCACGGCGTCGAGCCTGTGCGTGAAAAAGGGGGGCG
CGCACGCCAGATCGGGCGGACAAAAGGCGGCATGAACACCAAACTGCACGCCGTTGCCGATGCCACGGGGCGGCCGATCGGGTTCTTCATGTCGGCCGGC
GAGGTCAGCGACTACACAGGCGCCGCGGCTCTTCTGGGCAGTCTGCCGAAGGCGGGTTGGCTGCTGGCTGACAGGGGCTACGACGCTGATTGGTTCAGAG
AAGCGTTGAAAGACAGCGGGATAAAGGTCTGCATCCCCGGTCGAAAGTCCCGCAGGAAGGCCGTCAAATACGACAAGCGGCGCTACAAACGGCGCAACCG
CATCGAGATCATGTTCGGCCGTCTGAAGGATTGGCGGCGCGTCGCCACCCGATACGACCGCTGCCCGGAAACCTTCTTCTCCGCGATCATGCTCGCCGCA
ACCGTCTTGTTCTGGCTGTGAAATTCAATGAGTCTGGACCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 372 bp | 123 aa | 64 | 435 | + | No |
Description : First part of the transposase
ORF sequence :
MSNLYWLSDAQMERLKPFFPKSHGKPRVDDRRVLSRIIFINCNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIMVGLAAESADHKTIMIDATYLKAH
RTASSLCVKKGGAHARSGGQKAA
RTASSLCVKKGGAHARSGGQKAA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 312 | 821 | + | No |
Description : Second part of the transposase
ORF sequence :
ERRSQDDHDRCDLSQGTPHGVEPVREKGGRARQIGRTKGGMNTKLHAVADATGRPIGFFMSAGEVSDYTGAAALLGSLPKAGWLLADRGYDADWFREALK
DSGIKVCIPGRKSRRKAVKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPETFFSAIMLAATVLFWL
DSGIKVCIPGRKSRRKAVKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPETFFSAIMLAATVLFWL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 758 bp | 252 aa | 64 | 821 | + | Yes |
Chemistry : DDE
ORF sequence :
MSNLYWLSDAQMERLKPFFPKSHGKPRVDDRRVLSRIIFINCNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIMVGLAAESADHKTIMIDATYLKAH
RTASSLCVKKGGRARQIGRTKGGMNTKLHAVADATGRPIGFFMSAGEVSDYTGAAALLGSLPKAGWLLADRGYDADWFREALKDSGIKVCIPGRKSRRKA
VKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPETFFSAIMLAATVLFWL
RTASSLCVKKGGRARQIGRTKGGMNTKLHAVADATGRPIGFFMSAGEVSDYTGAAALLGSLPKAGWLLADRGYDADWFREALKDSGIKVCIPGRKSRRKA
VKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPETFFSAIMLAATVLFWL
Blast result :
Comments
ISPye49 is 97% aa similar to ISPaes2.
ISPye49 was identified by in silico sequence analysis of Paracoccus yeei CCUG 32053 (1 copy, reconstructed - disrupted by insertion of ISPye48 into IRR) and Paracoccus zhejiangensis strain J6 (1 isoform).
The third ORF is the putative ORFAB transposase reconstructed by possible -1 frameshift.
ISPye49 was identified by in silico sequence analysis of Paracoccus yeei CCUG 32053 (1 copy, reconstructed - disrupted by insertion of ISPye48 into IRR) and Paracoccus zhejiangensis strain J6 (1 isoform).
The third ORF is the putative ORFAB transposase reconstructed by possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.