ISPye48
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei CCUG 32053 |
DNA section
IS Length : 1457 bp
Ends
IR Length : 10
IRL : ATGCCGTAACGGGGCCTGCGTCGCGACCCACGTGTTACCGTTGCCAAGCC
IRR : TCGGTAACGGGGCTGTCTGGGTCAAGGAGTTTTCGACAGACGGTCCCGGA
Comments : Insertion within another IS enables precisely defining the ends and DRs. IRs are not at the ends.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| aaattcaatg | AG | tctggaccct | 2 |
DNA sequence
ATGCCGTAACGGGGCCTGCGTCGCGACCCACGTGTTACCGTTGCCAAGCCGAGGGCAAGCTGGCGCCATTCCCGTATCAGATGCATTGGGAGGACTTCGG
GATGAAGCTGTTTGTCGGACTGGACGTGTCGCTTGAGAAGACCGCAATCTGCGTTGTCAGCGAACATGGGAAAATCGAGAGGGAGGCGCAGGCGGCCAGC
GAGCCGGAAGCGCTGACGCGTTGGATGGGGCACCTCGACGGCACTATCGCCGCCATCGGCCTTGAGGCTGGCCCCCTGTCGCAATGGCTGTATCGCGGGC
TGACCGAGGCGGGTCTCGACGTCGTGCTGATGGAAACCCGGCAGGTGAAAGGCGCTCTGAAGGCCATGCCGATCAAGACCGATCGGCGGGATGCTGAGGG
GATAGCACGCCTCCTGCACCTCGGCTGGTTCCGGCCGGTCCACTGTAAATCCGTTTCGGCACAGGAGGTTCGTGCTGTGCTGGGCGCCCGCAAGGCGGTT
CAGCAGGGCATGATCGCCTTGGAGATGTCGCTGCGAGGGCTCCTGCGAAACTTCGGCCTGAAGGTCGGTGCGATCTCCCGCGGCCGGTTCGAGGCCCGTA
TCCGGGAACTCGCGACGGGCAATCCCATGCTCGTGGCCGCCACTGAGCCTTTGCTTCGGGCGCGTGCCGCCTTGCGACAGGAACTGGCCGGCCTAGAAAG
GCGGGTCCGTCAACTCGCGCAAGACGACCCTGTCTGTCATCGGTTGATGTCAATGCCAGGGATCGGCGCGGTCGTGGCACTGACATTCCGCTCGGCGGTG
GATGACCCCGCCCGGTTCACTTCCTCGAAGAAGGTCGGGCCATGGGTCGGCCTGACACCCTCGCGCAATCAGTCCGGTGAGCGGGACGTGTCGGGCGGGA
TCACCAAGGCGGGCGACGTGAACCTGCGCCGGGCCTTGTGCCAGGCAGCGACCGTTATGATGCACCGCGGGCGGGCGACTTGGCTGAGGGCATGGGCGGC
ACAGGTTGCTCGTCGTCGTGGCCGGAGGCGTGCGATGGTTGCGCTGGCGCGGCGGATCGGCGTGATCCTGCATCGGATGTGGCGTGATGGCACCGTATTC
TGGATTGAGCCATCGGTCACCTCCCATGCGGCGTAAACCTCTGAAGCTTTATCTGTTGCCTGCCTGACCGCAGGCCTTCGAGGTCCCCCAGGGACGCGGT
TCCGACGATGTCGTGGTCTAGACTGTTGCCGGCCGACAAGCTCGAGCACGCCAATGAGATGGGCACGTCGGAATTGCATCGAACCAGCATCATGTTGGCA
GCCACGCGCTGACCACGGACCGAAGCATGCACCCGGGGTGATCACAACCAAAGGCCGCATCCGCAGAATGGCGACGAAAGTCATCTTGCTATCGCCGCCC
TTGGGCGTCCGGGACCGTCTGTCGAAAACTCCTTGACCCAGACAGCCCCGTTACCGA
GATGAAGCTGTTTGTCGGACTGGACGTGTCGCTTGAGAAGACCGCAATCTGCGTTGTCAGCGAACATGGGAAAATCGAGAGGGAGGCGCAGGCGGCCAGC
GAGCCGGAAGCGCTGACGCGTTGGATGGGGCACCTCGACGGCACTATCGCCGCCATCGGCCTTGAGGCTGGCCCCCTGTCGCAATGGCTGTATCGCGGGC
TGACCGAGGCGGGTCTCGACGTCGTGCTGATGGAAACCCGGCAGGTGAAAGGCGCTCTGAAGGCCATGCCGATCAAGACCGATCGGCGGGATGCTGAGGG
GATAGCACGCCTCCTGCACCTCGGCTGGTTCCGGCCGGTCCACTGTAAATCCGTTTCGGCACAGGAGGTTCGTGCTGTGCTGGGCGCCCGCAAGGCGGTT
CAGCAGGGCATGATCGCCTTGGAGATGTCGCTGCGAGGGCTCCTGCGAAACTTCGGCCTGAAGGTCGGTGCGATCTCCCGCGGCCGGTTCGAGGCCCGTA
TCCGGGAACTCGCGACGGGCAATCCCATGCTCGTGGCCGCCACTGAGCCTTTGCTTCGGGCGCGTGCCGCCTTGCGACAGGAACTGGCCGGCCTAGAAAG
GCGGGTCCGTCAACTCGCGCAAGACGACCCTGTCTGTCATCGGTTGATGTCAATGCCAGGGATCGGCGCGGTCGTGGCACTGACATTCCGCTCGGCGGTG
GATGACCCCGCCCGGTTCACTTCCTCGAAGAAGGTCGGGCCATGGGTCGGCCTGACACCCTCGCGCAATCAGTCCGGTGAGCGGGACGTGTCGGGCGGGA
TCACCAAGGCGGGCGACGTGAACCTGCGCCGGGCCTTGTGCCAGGCAGCGACCGTTATGATGCACCGCGGGCGGGCGACTTGGCTGAGGGCATGGGCGGC
ACAGGTTGCTCGTCGTCGTGGCCGGAGGCGTGCGATGGTTGCGCTGGCGCGGCGGATCGGCGTGATCCTGCATCGGATGTGGCGTGATGGCACCGTATTC
TGGATTGAGCCATCGGTCACCTCCCATGCGGCGTAAACCTCTGAAGCTTTATCTGTTGCCTGCCTGACCGCAGGCCTTCGAGGTCCCCCAGGGACGCGGT
TCCGACGATGTCGTGGTCTAGACTGTTGCCGGCCGACAAGCTCGAGCACGCCAATGAGATGGGCACGTCGGAATTGCATCGAACCAGCATCATGTTGGCA
GCCACGCGCTGACCACGGACCGAAGCATGCACCCGGGGTGATCACAACCAAAGGCCGCATCCGCAGAATGGCGACGAAAGTCATCTTGCTATCGCCGCCC
TTGGGCGTCCGGGACCGTCTGTCGAAAACTCCTTGACCCAGACAGCCCCGTTACCGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1035 bp | 344 aa | 102 | 1136 | + | No |
Chemistry : DEDD
ORF sequence :
MKLFVGLDVSLEKTAICVVSEHGKIEREAQAASEPEALTRWMGHLDGTIAAIGLEAGPLSQWLYRGLTEAGLDVVLMETRQVKGALKAMPIKTDRRDAEG
IARLLHLGWFRPVHCKSVSAQEVRAVLGARKAVQQGMIALEMSLRGLLRNFGLKVGAISRGRFEARIRELATGNPMLVAATEPLLRARAALRQELAGLER
RVRQLAQDDPVCHRLMSMPGIGAVVALTFRSAVDDPARFTSSKKVGPWVGLTPSRNQSGERDVSGGITKAGDVNLRRALCQAATVMMHRGRATWLRAWAA
QVARRRGRRRAMVALARRIGVILHRMWRDGTVFWIEPSVTSHAA
IARLLHLGWFRPVHCKSVSAQEVRAVLGARKAVQQGMIALEMSLRGLLRNFGLKVGAISRGRFEARIRELATGNPMLVAATEPLLRARAALRQELAGLER
RVRQLAQDDPVCHRLMSMPGIGAVVALTFRSAVDDPARFTSSKKVGPWVGLTPSRNQSGERDVSGGITKAGDVNLRRALCQAATVMMHRGRATWLRAWAA
QVARRRGRRRAMVALARRIGVILHRMWRDGTVFWIEPSVTSHAA
Blast result :
Comments
ISPye48 is 93% aa similar to ISPye25.
ISPye48 was identified by in silico sequence analysis of Paracoccus yeei CCUG 32053 (1 copy, inserted within the IRR of another IS).
ISPye48 was identified by in silico sequence analysis of Paracoccus yeei CCUG 32053 (1 copy, inserted within the IRR of another IS).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.