ISPye45

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Paracoccus yeei
Paracoccus yeei
Paracoccus yeei CCUG 32053
DNA section
IS Length : 2462 bp

Ends


IR Length : 14/20

IRL : GTAAGCGTTTCCTGGCCTCCACCTTCCCGTCTTTGACCTGATCATCGCAT
IRR : GTAAGCGGTCGCTGGAAACCGCGGTCGGCTTAGCTTGACGGCTTGAAGTT

Comments : Empty insertion sites in other P. yeei strains enabled precisely defining ends and DRs.

Insertion site


Left flankDirect repeatRight flankDR Length
ttccggccgaAGACAGCCtgcctgaagg8
cacggcaggaGTATGTATgaagaagata8
ctgagctactCCCCAATCcttggaactt8
gccctcttgtgtcttggcgg0

DNA sequence

GTAAGCGTTTCCTGGCCTCCACCTTCCCGTCTTTGACCTGATCATCGCATTGGATGTTTGTGATGATCAGGACGGAGTTTCTCCATGGAGGCTGCATTGG
AGGTTCTCCCGGTTGGTGGCCGAAGCCGCCGGAACCGGAAGTGGCCAGATGAGGTGAAGGCGCGGGTTGTGGCGGAAACGCTGCTGCCGGGCGCAACGGT
GAACGAAGTGGCGCGCCGGCACGGGGTTTGCGCGAACCATGTTTCGTCGTGGCGGACGCTGGCGCGGAAAGGGCGGTTGGTCCTGCCCGCGCCGGAGGAT
CCGGTGGAATTTGTGGCGCTGATGGTCGGCTCCACTGAGGATGCGCCGCGCGCTGATGTTGGCGGCGAGGCCCGGCCGGAGATCATCGCCGGCACCGTTG
TGATCCGCCTGGAAACCGGGGCCACGGCGGAGCGGATTGCCTCGGTCGCGCATGCGTTGGCAGCGCGGCCATGATGTTTCCGTCGAACCGGGTGCGGATC
ATGGTCGCGACAAAGCCCGTCGACTTCAGGAAGGGACATGACGGCTTGTGTGCGCTGGTTGCCTCGATGTTGCGCAAGGATCCGTTCACCGGCACGGTGT
TCGTGTTCCGCTCGCGGCGGCTGGACAGGCTGAAGCTGCTTTACTGGGACGGCACCGGTCTGGTGATGGCCTATAAGCGGCTGGAGGCCACGAGTTTCAC
CTGGCCCGCGATCAAGGACGGGGTGATTGCGCTGAACCATGCGCAGTTCGAGGCGCTGTTTGCCGGGCTGGACTGGCGCAAGGTCAAGGCATTGGATGTG
CGCCCGCCTGCTGCGACAGAATGAATCAACCGGTTGTTTTTTCGTGTTTTTGCTGAGGTTTGATGATAGTGTCAGGCCATGCAGGCCGTCTCCTCTCGAT
CTGTCCGCCATCCCGACTGCGCAACGCGCGGCGGTTCAGGCATTGATGGAGCAGGTGGCGGCACTCACGGAAATCACCCGGCGGCAAGAGCACCTGATCG
CTGAGTTGAATCATGCCCTGCATGGCAAGCGGTCAGAAAAGCTGACCGAGGACGAGCGGCAGCTGGCCTTCGAGGACTTATCCATCGCGCTGGCCGAGGT
CGAGGCCGAGAAAGAAACCCGCTCTGCCAAAGGGGGCGGTGGGGCAGCCAGGCCCGCGCCAAAGCGCACCATCGGCAACCTCCCGGCCGCGCTGCCCCGC
ATCGAGGAAGTCATCGAGCCTGACAGCCTGATCTGCCCCTGTGGCTGCGGCATCATGCACAAGATCGGCGAAGATCGCTCGGAGCGGCTGGACATCGTGC
CGGCTCAGTTGCGGGTCATCGTCACCGTGCGGCCGAAGTATGCTTGCCGGACTTGCACCGACGGTGTGACCCAGGCGCCCGCGCCGTCCCACCTGATCAC
GGGCGGCCTGCCGACCGAGGCAACCATCGCCCATGTGCTGGTCAGCAAATATGCGGACCATCTGCCTCTGTATCGCCAGAGCCAGATCCTGGCGCGGGCA
GGCCTTGATCTGCACCGCGCCGTGCTGGCTGATTGGGTCGGCAAGGCTGCATTCCATCTGAAGCCCGTCGTCGACCGGCTGGCCGAGCATCTGAAACGAT
CCGGCAAGCTGTTCATGGACGAAACTACCGCCCCGGTGCTGGATCCGGGGCGCGGCACGACCAAGACTGGATATCTCTGGGCCTTTGGCCGCGATGACCG
ACCATGGGGCGGGGACGATCCGCCCGGCGTGGTCTACTTCTACGCGCCCGGTCGCGCCGGCGAGAATGCCGAAACCTTCCTGACCGGCTTCGACGGTATC
CTGCAAGTCGATGGGTATCCCGGCTACAACCGGCTGACTAAAGCCTCGCGCAAGGGCGGCGATCCCATTCGGGTGGCCCATTGCTGGGCGCATGCACGGC
GCAAGCTGAAAGAGGTCTTCGACCGCGACGGCTCCGAGATCGCCGCCGAGGGCCTGCGCCGCATCGCCGAATTCTATGCCGTCGAGGCCGATATCCGCGG
CGTCTCGCCCGGCCAACGCCTGTCTGCCCGCCAGGCCCGCACCGCGCCGCTGGTGGCAGCCTTCGGCGGCTGGCTTCAGGCGCAGCGCCGCAAGATATCC
GCCAAATCCCGCCTGGGCGAAAAGCTGACCTACATCCATAACCAATGGGACGGGCTGCAAACCTTCCTGACCGACGGCCGCGTCGAGATCGACTCCAACA
GGGTCGAGAATCTCGTCCGCCCGATCGCCCTCAATCGCAAGAATGCGCTCTTCGCCGGTCACGACGAAGGCGGTATCGCCTGGGGGCGCATCGCCTCGCT
GATCGAAACCTGCAAGATCAATGGCGTCGAGCCCTTCGCCTACCTCAACGCCACGCTCACCGCCATCGCCAGCGGTCATCCGCAAAGCCGCATCGACGAC
CTGCTGCCATGGAACTTCAAGCCGTCAAGCTAAGCCGACCGCGGTTTCCAGCGACCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
390 bp129 aa85474+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MEAALEVLPVGGRSRRNRKWPDEVKARVVAETLLPGATVNEVARRHGVCANHVSSWRTLARKGRLVLPAPEDPVEFVALMVGSTEDAPRADVGGEARPEI
IAGTVVIRLETGATAERIASVAHALAARP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
354 bp117 aa471824+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MMFPSNRVRIMVATKPVDFRKGHDGLCALVASMLRKDPFTGTVFVFRSRRLDRLKLLYWDGTGLVMAYKRLEATSFTWPAIKDGVIALNHAQFEALFAGL
DWRKVKALDVRPPAATE

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1488 bp495 aa9462433+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEQVAALTEITRRQEHLIAELNHALHGKRSEKLTEDERQLAFEDLSIALAEVEAEKETRSAKGGGGAARPAPKRTIGNLPAALPRIEEVIEPDSLICPCG
CGIMHKIGEDRSERLDIVPAQLRVIVTVRPKYACRTCTDGVTQAPAPSHLITGGLPTEATIAHVLVSKYADHLPLYRQSQILARAGLDLHRAVLADWVGK
AAFHLKPVVDRLAEHLKRSGKLFMDETTAPVLDPGRGTTKTGYLWAFGRDDRPWGGDDPPGVVYFYAPGRAGENAETFLTGFDGILQVDGYPGYNRLTKA
SRKGGDPIRVAHCWAHARRKLKEVFDRDGSEIAAEGLRRIAEFYAVEADIRGVSPGQRLSARQARTAPLVAAFGGWLQAQRRKISAKSRLGEKLTYIHNQ
WDGLQTFLTDGRVEIDSNRVENLVRPIALNRKNALFAGHDEGGIAWGRIASLIETCKINGVEPFAYLNATLTAIASGHPQSRIDDLLPWNFKPSS

 

Blast result :
Comments
ISPye45 is 72% aa similar to ISRel8.
ISPye45 was identified by in silico sequence analysis of Paracoccus yeei strain CCUG 32053 (4 copies of ISPye45 and isoforms present in the genome).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission