ISPye41
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei CCUG 32053 |
DNA section
IS Length : 1055 bp
Ends
IR Length : 20/23
IRL : GGCGTTGTTGCAAAACTCTGCGTGCCGCGGGATTCACGCGGCGGGGCTGG
IRR : GGCATTGTTGCAGAACTCGGCGTTGAAGCGTGACGTCCCCTTACCCCACC
Comments : Insertion in an entrapment vector pMEC1 enabled precisely defining ends and DRs. Additionally, for many copies we identified empty insertion sites in other P. yeei strains.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ggcgcataca | TCCAGAG | cgctccattc | 7 |
| ggtccagacc | cctccgccgg | 0 | |
| ccatccatcc | cctccgccgg | 0 | |
| cctgcgcttg | atcaggcgca | 0 | |
| gcgaagatgg | TTTCCGAAC | tcgcaacctc | 9 |
| gaaaatggca | AGGTGAATG | tcacctcgct | 9 |
| gataccttgc | gagtagttcg | 0 | |
| agcacgaatg | gagatgttta | 0 | |
| gggtcctgac | ggctctgtca | 0 | |
| tcgtcaactg | gctgtaatcc | 0 | |
| ggtgaaacct | tttccgatcc | 0 | |
| gcagcttagt | ggtaccgtcc | 0 | |
| AGAATTTAGC | CCTTCAATC | GCCAGAGAAA | 9 |
DNA sequence
GGCGTTGTTGCAAAACTCTGCGTGCCGCGGGATTCACGCGGCGGGGCTGGTAGAGTAAGCGTTTCGGCATGAGCAAGCCCGAAGCTGTCCGCTACCGCAC
GACGAACTGGAAGTCCTACAACAAGGCCCTGAAGCGGCGCGGGTCACTGCTGATCTGGCTGGACAAGGACATGGACTGGCTGCCCCCCAAGGCGGGGCGT
CCTGGTCGTCCGCCGGTGTTCTCTGATGCAGCGATCCAGTTCTGCCTGATGGTGAAGGTGCTCTTTGGCCTGCCACTCCGGCAAACAACCGGGATGGTGG
CCAGCATTCTGAAGATGGCAGGGCTCGACTGGCCGGTGCCGGACTTCTCCACCCTGAGCCGCAGACAAGGATCTTTGGTGGTCGACATACCCCACCGCCG
TGCAACGGGGCCGCTGAACCTGCTGGTCGACAGCACCGGGATCAAGTTCCTGGGTGACGGGGAATGGCTGGCGCGCAAGCACGGCACGCACCGCAGGCGC
CAATACCGCAAGGTTCATCTGGCGATGGACACGGCCACCGGCGACATCCGAGCGGTGGAGTTCACCTCGAGCCGCGAAGGCGACAGCCCTGTGCTGCCGG
ATCTGCTGGATCAGATCCCGGCCGATGAACAGGTCGGCACCGTGACCGGCGACGGCGCCTTCGACACCCGGCGGTGTCACACCGCAATCGTCGATCGCGG
CGGCACGGCCATCATCCCGATCCGCAAGAACGGACGCCTCTGGAAGGAGAACTGCCCTGCCGCCCGTGCCCGCAACGACATCCTTCGGGCGAGCAAACGC
TTTGGGCGAGCCAACTGGAAGCACTGGTCCGGCTATCACGTCCGAAGTCGGATCGAGGCAAAAATGCGCTGCCTGAAGTCCTTCGGCGAGCGCATCGCCT
CACGAGACCCCGACCGCCAGACTGCTGAGATCCACATCCGCATCGCCCTCATGAACCGCTTCAACACCCTCGGCACTGCCGAGACCGAACGCGTGGCCTA
AGGCTGGTGGGGTAAGGGGACGTCACGCTTCAACGCCGAGTTCTGCAACAATGCC
GACGAACTGGAAGTCCTACAACAAGGCCCTGAAGCGGCGCGGGTCACTGCTGATCTGGCTGGACAAGGACATGGACTGGCTGCCCCCCAAGGCGGGGCGT
CCTGGTCGTCCGCCGGTGTTCTCTGATGCAGCGATCCAGTTCTGCCTGATGGTGAAGGTGCTCTTTGGCCTGCCACTCCGGCAAACAACCGGGATGGTGG
CCAGCATTCTGAAGATGGCAGGGCTCGACTGGCCGGTGCCGGACTTCTCCACCCTGAGCCGCAGACAAGGATCTTTGGTGGTCGACATACCCCACCGCCG
TGCAACGGGGCCGCTGAACCTGCTGGTCGACAGCACCGGGATCAAGTTCCTGGGTGACGGGGAATGGCTGGCGCGCAAGCACGGCACGCACCGCAGGCGC
CAATACCGCAAGGTTCATCTGGCGATGGACACGGCCACCGGCGACATCCGAGCGGTGGAGTTCACCTCGAGCCGCGAAGGCGACAGCCCTGTGCTGCCGG
ATCTGCTGGATCAGATCCCGGCCGATGAACAGGTCGGCACCGTGACCGGCGACGGCGCCTTCGACACCCGGCGGTGTCACACCGCAATCGTCGATCGCGG
CGGCACGGCCATCATCCCGATCCGCAAGAACGGACGCCTCTGGAAGGAGAACTGCCCTGCCGCCCGTGCCCGCAACGACATCCTTCGGGCGAGCAAACGC
TTTGGGCGAGCCAACTGGAAGCACTGGTCCGGCTATCACGTCCGAAGTCGGATCGAGGCAAAAATGCGCTGCCTGAAGTCCTTCGGCGAGCGCATCGCCT
CACGAGACCCCGACCGCCAGACTGCTGAGATCCACATCCGCATCGCCCTCATGAACCGCTTCAACACCCTCGGCACTGCCGAGACCGAACGCGTGGCCTA
AGGCTGGTGGGGTAAGGGGACGTCACGCTTCAACGCCGAGTTCTGCAACAATGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 69 | 1001 | + | No |
Chemistry : DDE
ORF sequence :
MSKPEAVRYRTTNWKSYNKALKRRGSLLIWLDKDMDWLPPKAGRPGRPPVFSDAAIQFCLMVKVLFGLPLRQTTGMVASILKMAGLDWPVPDFSTLSRRQ
GSLVVDIPHRRATGPLNLLVDSTGIKFLGDGEWLARKHGTHRRRQYRKVHLAMDTATGDIRAVEFTSSREGDSPVLPDLLDQIPADEQVGTVTGDGAFDT
RRCHTAIVDRGGTAIIPIRKNGRLWKENCPAARARNDILRASKRFGRANWKHWSGYHVRSRIEAKMRCLKSFGERIASRDPDRQTAEIHIRIALMNRFNT
LGTAETERVA
GSLVVDIPHRRATGPLNLLVDSTGIKFLGDGEWLARKHGTHRRRQYRKVHLAMDTATGDIRAVEFTSSREGDSPVLPDLLDQIPADEQVGTVTGDGAFDT
RRCHTAIVDRGGTAIIPIRKNGRLWKENCPAARARNDILRASKRFGRANWKHWSGYHVRSRIEAKMRCLKSFGERIASRDPDRQTAEIHIRIALMNRFNT
LGTAETERVA
Blast result :
Comments
ISPye41 is 94% aa similar to ISPth1.
ISPye41 was identified by in silico sequence analysis of Paracoccus yeei strain CCUG 32053 (12 copies of ISPye41 and isoforms). Additionally, ISPye41 has been identified by its transposition into an entrapment vector pMEC1 (cI-tetA) in P. yeei CCUG 32053.
ISPye41 was identified by in silico sequence analysis of Paracoccus yeei strain CCUG 32053 (12 copies of ISPye41 and isoforms). Additionally, ISPye41 has been identified by its transposition into an entrapment vector pMEC1 (cI-tetA) in P. yeei CCUG 32053.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.