ISLmo8

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP006611 ND Listeria monocytogenes
Listeria monocytogenes
Listeria monocytogenes N1-011A
Listeria monocytogenes R2-502
Listeria monocytogenes CFSAN010068
DNA section
IS Length : 1257 bp

Ends


IR Length : 26/38

IRL : TGGATGTAGGATAGAAGTTGAGACAACTTTTAAGAGAGGGTAAAATTAAT
IRR : TGGATCAGAGTCAATAGTTGAAACAGTTTTTACGAGAGACTTTTAAGATT

Insertion site


Left flankDirect repeatRight flankDR Length
atatttaaagctcctagtaatcc3

DNA sequence

TGGATGTAGGATAGAAGTTGAGACAACTTTTAAGAGAGGGTAAAATTAATCTCAAAGGAAGGTGTCTTACATGCCCAAACGCTATACAGAAGATTTTAAA
CAAACGATACTTGATCTTCATGAACAAGGACAAACACCCCGGCAACTGGCTGCTGATTACGAAGTTGGTTACTCCACCATTTTGAAGTGGATTCAAGGGA
ATACACCTATCAGTCCCGGAGGGCCTACTTCTAACGAAGTCAAACGATTAAAAAAACAATTGAAAGAAAAGGATGAAGAACTCCTCATCTTAAAAAAAGC
ACTCGGTCTGCTCGCAAAGAAATAGCCATCCTAGAATGGATGAACCAAGAACAAGAAGCAGGCCGCCATAGCATTTTAAAAATGTGTACGAGTCTTGGTG
TTTCCAGAAGTTCCTACTATAGGCATTTAAATCTGATTGTTTCCAAAAGAAAGCGAGCCGATCAGTTGTTGGATCAACGTGTTTTACGATTGTATCAAGA
AAATCAAGGAATCTATGGAGCTGGGAAACTTCGTTATTTGTTAGCAAGTTCGTACCACGAATATCCGGACATCAGTATCAAACGTGTCCAAAAATCAATG
AGAAGACAAAACCTGCGGTCTATCACGGTAAAGAAATTCAAAGCAGGAAGACCGACGAAAAGGATCTTGAAAACTTATAAAAATCTATTGAATCAAGATT
TTTCAACGACTGGACTCAATCAAAAATGGGTAGCTGATATTACTTATATCTATACTCTCGCAGACGGCTGGTGCTATTTATCCACCATCATGGATTTGCA
TTCCCGAAAAATTATTGGGTATCACTTTGATCGAACAATGCAGACTTCTATCGTCCTTAAAGCCCTAGATGATGCATTTTCTAAACGAAAGATAGAAAAA
GGACTGATCCTTCATACCGATTTAGGTTCGCAATATACAAGTATCGACTATGAAACAAAGTTAAAAGCAATGAATCTCTGCCATTCATACAGCCAAAAAG
GCTGTCCGTATGATAACGCTGGAATCGAGTCTTTCCATGCGCTGCTAAAGAAAGAGCATGTTTACCAACGATCTGCTTATTCCAATTTTGAAGAAGCAAA
GCTTCAAGTTTTCAGCTATGTGCAAGGCTTCTATAACAATCGCCGAATTCATAGCTCATTAGCTTATTTATCCCCAAACAAGTTTGAACAGAAAATTTCA
GCTGCTTAATCTTAAAAGTCTCTCGTAAAAACTGTTTCAACTATTGACTCTGATCCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
255 bp84 aa71325+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPKRYTEDFKQTILDLHEQGQTPRQLAADYEVGYSTILKWIQGNTPISPGGPTSNEVKRLKKQLKEKDEELLILKKALGLLAKK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
933 bp310 aa2771209+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RTPHLKKSTRSARKEIAILEWMNQEQEAGRHSILKMCTSLGVSRSSYYRHLNLIVSKRKRADQLLDQRVLRLYQENQGIYGAGKLRYLLASSYHEYPDIS
IKRVQKSMRRQNLRSITVKKFKAGRPTKRILKTYKNLLNQDFSTTGLNQKWVADITYIYTLADGWCYLSTIMDLHSRKIIGYHFDRTMQTSIVLKALDDA
FSKRKIEKGLILHTDLGSQYTSIDYETKLKAMNLCHSYSQKGCPYDNAGIESFHALLKKEHVYQRSAYSNFEEAKLQVFSYVQGFYNNRRIHSSLAYLSP
NKFEQKISAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1139 bp379 aa711209+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKRYTEDFKQTILDLHEQGQTPRQLAADYEVGYSTILKWIQGNTPISPGGPTSNEVKRLKKQLKEKDEELLILKKSTRSARKEIAILEWMNQEQEAGRH
SILKMCTSLGVSRSSYYRHLNLIVSKRKRADQLLDQRVLRLYQENQGIYGAGKLRYLLASSYHEYPDISIKRVQKSMRRQNLRSITVKKFKAGRPTKRIL
KTYKNLLNQDFSTTGLNQKWVADITYIYTLADGWCYLSTIMDLHSRKIIGYHFDRTMQTSIVLKALDDAFSKRKIEKGLILHTDLGSQYTSIDYETKLKA
MNLCHSYSQKGCPYDNAGIESFHALLKKEHVYQRSAYSNFEEAKLQVFSYVQGFYNNRRIHSSLAYLSPNKFEQKISAA

 

Blast result :
Comments
ISLmo8 is 70% aa similar to ISBce13.
ISLmo8 was identified by in silico sequence analysis of Listeria monocytogenes strain N1-011A plasmid. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska, C. and Bartosik, D.(2018) Direct submission.