ISPye36
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei CCUG 32053 |
DNA section
IS Length : 1217 bp
Ends
IR Length : 19/25
IRL : TGGTCCTCCCCCACTGAAGTGGTCCGCCGTTATGTTTAGGAAACGGAGGA
IRR : TGATCAGCCCCACCTGAGTGGTCCATTTTGAAAGTTAGTGCATGACCAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gtggtccacc | ccgtgctggt | 0 |
DNA sequence
TGGTCCTCCCCCACTGAAGTGGTCCGCCGTTATGTTTAGGAAACGGAGGATCGAGAATGGCAAACAAACGACCGAAGCCGGAAGAGATTGTCTCGAAGTT
GCGGCAGGTTGAGGTTCTGATGGGGCAAGGCTTGTCCCGTCTGGACGCGATCAGGCGTATCGGCGTTGTCGAACAGACCTATTACCGCTGGCGCAAGCAA
TACGGCGGAATGGGCGTAGACCAGTTGAAAGAGCTGAAGCGCCTTCAGCAGGAGAATGAGCGGCTCAGGCGTGCGGTGTCTGACCTGACGTTGGACAAGC
TAATCCTGACGGAGGCGGCCCGGGGAAACTTCTGAGCCCCGCTCGCCGCCGGGCCTGTATTGACCATGTCCGCCGTGAGCTGGGCGTTTCCGAGCGTCGC
GCCTGCCAAGTGCTTCGCCAACATCGTTCGACACAACGCAAGTTCCCGAGAGGTCGAGCGGACGAGGTCCGCCTGGCCGCCGACATGATTGAGCTGGCCC
GCCAATATGGCCGTTATGGCTATCGCCGGATTGCGGCTCTGTTGCGGGATGCCGGGTGGCAGGTGAATGACAAGCGGGTTGAGCGCTTGTGGCGGCGAGA
GGGGCTGAAAGTGCCGATGAAACAGCCGAAGAAAGGGCGATTGTGGCTGAACGACGGATCCTGCGTCCGGTTGCGGCCGCAATACCGGGATCATGTCTGG
TCGTATGATTTTGTTCATTGCCGCACCGATGATGGCAAGGTGTTCCGGACGTTGAACATCCTTGATGAGTTCAGTCGCGAATGTCTGACGATCAAGGTGC
GGCGCAGGCTGAATTCGACAGACGTGATTGATGCGCTGACCGATCTGTTCATCTTGCGCGGCGTGCCCGCCTTCATCCGTTCCGACAACGGTCCGGAGTT
CATTGCCCTGGCCGTTCGTGACTGGATCGGGGCGGTCGGGGCAAAAACGGCTTACATCGAACCGGGATCGCCTTGGGAAAATGGCTATTGTGAGAGCTTC
AACGCCCGCTTCCGCGATGAAATGTTGAATGGGGAAGTTTTCTACAGCCTCCGTGAGGCACAGATCCTGATCGAACAGTGGAGGAAGCATTACAACACGA
AACGACCGCACAGCGCATTGGGCTATCGCCCACCAGCCCCAGAAGCCTTTGTGCCGGTGGACCAAAGGCTGGTCATGCACTAACTTTCAAAATGGACCAC
TCAGGTGGGGCTGATCA
GCGGCAGGTTGAGGTTCTGATGGGGCAAGGCTTGTCCCGTCTGGACGCGATCAGGCGTATCGGCGTTGTCGAACAGACCTATTACCGCTGGCGCAAGCAA
TACGGCGGAATGGGCGTAGACCAGTTGAAAGAGCTGAAGCGCCTTCAGCAGGAGAATGAGCGGCTCAGGCGTGCGGTGTCTGACCTGACGTTGGACAAGC
TAATCCTGACGGAGGCGGCCCGGGGAAACTTCTGAGCCCCGCTCGCCGCCGGGCCTGTATTGACCATGTCCGCCGTGAGCTGGGCGTTTCCGAGCGTCGC
GCCTGCCAAGTGCTTCGCCAACATCGTTCGACACAACGCAAGTTCCCGAGAGGTCGAGCGGACGAGGTCCGCCTGGCCGCCGACATGATTGAGCTGGCCC
GCCAATATGGCCGTTATGGCTATCGCCGGATTGCGGCTCTGTTGCGGGATGCCGGGTGGCAGGTGAATGACAAGCGGGTTGAGCGCTTGTGGCGGCGAGA
GGGGCTGAAAGTGCCGATGAAACAGCCGAAGAAAGGGCGATTGTGGCTGAACGACGGATCCTGCGTCCGGTTGCGGCCGCAATACCGGGATCATGTCTGG
TCGTATGATTTTGTTCATTGCCGCACCGATGATGGCAAGGTGTTCCGGACGTTGAACATCCTTGATGAGTTCAGTCGCGAATGTCTGACGATCAAGGTGC
GGCGCAGGCTGAATTCGACAGACGTGATTGATGCGCTGACCGATCTGTTCATCTTGCGCGGCGTGCCCGCCTTCATCCGTTCCGACAACGGTCCGGAGTT
CATTGCCCTGGCCGTTCGTGACTGGATCGGGGCGGTCGGGGCAAAAACGGCTTACATCGAACCGGGATCGCCTTGGGAAAATGGCTATTGTGAGAGCTTC
AACGCCCGCTTCCGCGATGAAATGTTGAATGGGGAAGTTTTCTACAGCCTCCGTGAGGCACAGATCCTGATCGAACAGTGGAGGAAGCATTACAACACGA
AACGACCGCACAGCGCATTGGGCTATCGCCCACCAGCCCCAGAAGCCTTTGTGCCGGTGGACCAAAGGCTGGTCATGCACTAACTTTCAAAATGGACCAC
TCAGGTGGGGCTGATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 57 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MANKRPKPEEIVSKLRQVEVLMGQGLSRLDAIRRIGVVEQTYYRWRKQYGGMGVDQLKELKRLQQENERLRRAVSDLTLDKLILTEAARGNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 897 bp | 299 aa | 284 | 1180 | + | No |
Description : Second part of the transposase
ORF sequence :
PDVGQANPDGGGPGKLLSPARRRACIDHVRRELGVSERRACQVLRQHRSTQRKFPRGRADEVRLAADMIELARQYGRYGYRRIAALLRDAGWQVNDKRVE
RLWRREGLKVPMKQPKKGRLWLNDGSCVRLRPQYRDHVWSYDFVHCRTDDGKVFRTLNILDEFSRECLTIKVRRRLNSTDVIDALTDLFILRGVPAFIRS
DNGPEFIALAVRDWIGAVGAKTAYIEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPEAFVPVDQRLVMH
RLWRREGLKVPMKQPKKGRLWLNDGSCVRLRPQYRDHVWSYDFVHCRTDDGKVFRTLNILDEFSRECLTIKVRRRLNSTDVIDALTDLFILRGVPAFIRS
DNGPEFIALAVRDWIGAVGAKTAYIEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPEAFVPVDQRLVMH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1124 bp | 375 aa | 57 | 1180 | + | Yes |
Chemistry : DDE
ORF sequence :
MANKRPKPEEIVSKLRQVEVLMGQGLSRLDAIRRIGVVEQTYYRWRKQYGGMGVDQLKELKRLQQENERLRRAVSDLTLDKLILTEAARGKLLSPARRRA
CIDHVRRELGVSERRACQVLRQHRSTQRKFPRGRADEVRLAADMIELARQYGRYGYRRIAALLRDAGWQVNDKRVERLWRREGLKVPMKQPKKGRLWLND
GSCVRLRPQYRDHVWSYDFVHCRTDDGKVFRTLNILDEFSRECLTIKVRRRLNSTDVIDALTDLFILRGVPAFIRSDNGPEFIALAVRDWIGAVGAKTAY
IEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPEAFVPVDQRLVMH
CIDHVRRELGVSERRACQVLRQHRSTQRKFPRGRADEVRLAADMIELARQYGRYGYRRIAALLRDAGWQVNDKRVERLWRREGLKVPMKQPKKGRLWLND
GSCVRLRPQYRDHVWSYDFVHCRTDDGKVFRTLNILDEFSRECLTIKVRRRLNSTDVIDALTDLFILRGVPAFIRSDNGPEFIALAVRDWIGAVGAKTAY
IEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPEAFVPVDQRLVMH
Blast result :
Comments
ISPye36 is 97% aa similar to ISPye6.
ISPye36 was identified by in silico sequence analysis of Paracoccus yeei strain CCUG 32053. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPye36 was identified by in silico sequence analysis of Paracoccus yeei strain CCUG 32053. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.