ISPye34
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020442 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 |
DNA section
IS Length : 1271 bp
Ends
IR Length : 16/19
IRL : TGACCTGCCCCGGGATTTTTCCTCCAGATGCGATTAGAGTCCGGCCCAAC
IRR : TGACCTGCCCCTGAACTTTCATCCAGCCGCGACCGGAGCCCGGTTAGTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tagtgcgggg | GCGG | taccataacc | 4 |
| acaagagtag | aacc | agcaccgaag | 4 |
| tgcaaagtaa | TCGC | gtgaagactg | 4 |
DNA sequence
TGACCTGCCCCGGGATTTTTCCTCCAGATGCGATTAGAGTCCGGCCCAACTTGAGGACGGACAATGAAGCGAACGAGATTCACGGACGAACAGATCATCG
GCATCCTGGCAGAGCATGAGGCAGGCGCGAAGTGCGCTGACCTGTGCCGGAAGCACGGCATGTCGGAAGGCACCTTCTACAACTGGAAGGCCAAGTTCGG
CGGCATGACGGTGTCGGAGGCGAAGCGGCTGAAGGGGCTCGAGGATGAGAACGCCAAGCTGAAGAAGCTGCTGGCCGAGCAGATGCTGGATCTGGCGGCG
ATGAAGGACCTGGTTGCAAAAAAGTGGTAGGGCCCGCCGTGAAGCGTGAAGCCGTTGCGTATCTTCGGGCCGAGCATGGCCTCTCGGAACGGCGGGCCTG
TCACATCGTCGGCGCGGATCGAACGATGATCCGCTATCGATCCCGACGCGCGCCGGATACGGCGTTACGCGGTCGGTTGCGGGACTTGGCCAACGAGCGC
CGGCGGTTCGGTTACCGGCGCTTGTTCGTATTGCTGCGCCGCGAGGGCGAGCCCTCGGGGATCAATCGGATCTATCGGCTCTACCGCGAAGAAGGGCTGA
CGGTGCGCAAGCGGAAGGCGCGGCGCAAGGCGGTCGGAACACGGGCCCCTATCCTGGTCGAGGCGCGGCCCAATGCACGCTGGTCGCTGGATTTCGTCCA
TGACCAGTTCGATTGCGGGCGAAGATTCCGGGTGCTGAACATCGTCGATGACGTGACCCGGGAATGTTTGGCGGCGATCCCGGACACCTCAATCTCGGGG
CGGCGCGTGGCACGAGAACTGGCCGCCCTGATCGAACGCCGCGGAAAGCCCGGGATGATCGTCTCTGACAACGGCACCGAACTGACCAGTAACGCGATCT
TAACCTTTGCCGCCGATCGCGACATTGAGTGGCATTACATCGCCCCGGGCAAGCCGATGCAGAACGGATTTGTGGAAAGTTTCAACGGGCGGATGCGGGA
CGAGCTGCTCAACGAGACCATGTTCCGTAACCTGGCCCATGCCCGTATCGTGATCGCCGCCTGGGCTGCCGACTACAACACCGAACGCCCGCACTCGGCC
TTGGACTACCAGACCCCGGCTGACTACGCGCGGACCCTGACCGCCGCAATCGCCCGCCCCGCTGCGCGAGATGAAAGCTCCGCGCGTCGGGCGATTGCTC
AACCTGCGCCAATTGGCGTAAACACTAACCGGGCTCCGGTCGCGGCTGGATGAAAGTTCAGGGGCAGGTCA
GCATCCTGGCAGAGCATGAGGCAGGCGCGAAGTGCGCTGACCTGTGCCGGAAGCACGGCATGTCGGAAGGCACCTTCTACAACTGGAAGGCCAAGTTCGG
CGGCATGACGGTGTCGGAGGCGAAGCGGCTGAAGGGGCTCGAGGATGAGAACGCCAAGCTGAAGAAGCTGCTGGCCGAGCAGATGCTGGATCTGGCGGCG
ATGAAGGACCTGGTTGCAAAAAAGTGGTAGGGCCCGCCGTGAAGCGTGAAGCCGTTGCGTATCTTCGGGCCGAGCATGGCCTCTCGGAACGGCGGGCCTG
TCACATCGTCGGCGCGGATCGAACGATGATCCGCTATCGATCCCGACGCGCGCCGGATACGGCGTTACGCGGTCGGTTGCGGGACTTGGCCAACGAGCGC
CGGCGGTTCGGTTACCGGCGCTTGTTCGTATTGCTGCGCCGCGAGGGCGAGCCCTCGGGGATCAATCGGATCTATCGGCTCTACCGCGAAGAAGGGCTGA
CGGTGCGCAAGCGGAAGGCGCGGCGCAAGGCGGTCGGAACACGGGCCCCTATCCTGGTCGAGGCGCGGCCCAATGCACGCTGGTCGCTGGATTTCGTCCA
TGACCAGTTCGATTGCGGGCGAAGATTCCGGGTGCTGAACATCGTCGATGACGTGACCCGGGAATGTTTGGCGGCGATCCCGGACACCTCAATCTCGGGG
CGGCGCGTGGCACGAGAACTGGCCGCCCTGATCGAACGCCGCGGAAAGCCCGGGATGATCGTCTCTGACAACGGCACCGAACTGACCAGTAACGCGATCT
TAACCTTTGCCGCCGATCGCGACATTGAGTGGCATTACATCGCCCCGGGCAAGCCGATGCAGAACGGATTTGTGGAAAGTTTCAACGGGCGGATGCGGGA
CGAGCTGCTCAACGAGACCATGTTCCGTAACCTGGCCCATGCCCGTATCGTGATCGCCGCCTGGGCTGCCGACTACAACACCGAACGCCCGCACTCGGCC
TTGGACTACCAGACCCCGGCTGACTACGCGCGGACCCTGACCGCCGCAATCGCCCGCCCCGCTGCGCGAGATGAAAGCTCCGCGCGTCGGGCGATTGCTC
AACCTGCGCCAATTGGCGTAAACACTAACCGGGCTCCGGTCGCGGCTGGATGAAAGTTCAGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 64 | 330 | + | No |
Description : First part of the transposase
ORF sequence :
MKRTRFTDEQIIGILAEHEAGAKCADLCRKHGMSEGTFYNWKAKFGGMTVSEAKRLKGLEDENAKLKKLLAEQMLDLAAMKDLVAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1005 bp | 334 aa | 249 | 1253 | + | No |
Description : Second part of the transposase
ORF sequence :
ERQAEEAAGRADAGSGGDEGPGCKKVVGPAVKREAVAYLRAEHGLSERRACHIVGADRTMIRYRSRRAPDTALRGRLRDLANERRRFGYRRLFVLLRREG
EPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVEARPNARWSLDFVHDQFDCGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELAALIERRGK
PGMIVSDNGTELTSNAILTFAADRDIEWHYIAPGKPMQNGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARTLTAA
IARPAARDESSARRAIAQPAPIGVNTNRAPVAAG
EPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVEARPNARWSLDFVHDQFDCGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELAALIERRGK
PGMIVSDNGTELTSNAILTFAADRDIEWHYIAPGKPMQNGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARTLTAA
IARPAARDESSARRAIAQPAPIGVNTNRAPVAAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 396 aa | 64 | 1253 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRTRFTDEQIIGILAEHEAGAKCADLCRKHGMSEGTFYNWKAKFGGMTVSEAKRLKGLEDENAKLKKLLAEQMLDLAAMKDLVAKKVVGPAVKREAVAY
LRAEHGLSERRACHIVGADRTMIRYRSRRAPDTALRGRLRDLANERRRFGYRRLFVLLRREGEPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVE
ARPNARWSLDFVHDQFDCGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELAALIERRGKPGMIVSDNGTELTSNAILTFAADRDIEWHYIAPGKPMQ
NGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARTLTAAIARPAARDESSARRAIAQPAPIGVNTNRAPVAAG
LRAEHGLSERRACHIVGADRTMIRYRSRRAPDTALRGRLRDLANERRRFGYRRLFVLLRREGEPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVE
ARPNARWSLDFVHDQFDCGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELAALIERRGKPGMIVSDNGTELTSNAILTFAADRDIEWHYIAPGKPMQ
NGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARTLTAAIARPAARDESSARRAIAQPAPIGVNTNRAPVAAG
Blast result :
Comments
ISPye34 is 97% aa similar to ISOba1.
ISPye34 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. There are 3 reconstructed copies disrupted by insertion of ISPye16 (IS110 family) in the same position - possibly a mosaic transposable element. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPye34 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. There are 3 reconstructed copies disrupted by insertion of ISPye16 (IS110 family) in the same position - possibly a mosaic transposable element. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.