ISfinder Mirror Site

ISPye33

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP020442	ND	Paracoccus yeei	Paracoccus yeei Paracoccus yeei FDAARGOS_252

DNA section

IS Length : 2366 bp

Ends

IR Length : 19/22

IRL : GTAACCGTCCGGTCTAACCGCTCTGCCGCGGTGTGAGAGTGCGGGATAGT
IRR : GTAACCGTCCGGCGTGACCGCTATCCGTTCCAGCGCCACGGCATCAGGTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
cgtcctcgat	GATGAGGG	ggatgagatc	8

DNA sequence

GTAACCGTCCGGTCTAACCGCTCTGCCGCGGTGTGAGAGTGCGGGATAGTGTCCACCATTGATAGTGGACACTGTGGTGATGACGTGGCGCGTCGGACGA
AGCGGCTTTGGACGGATGAGGAGAAGCGGTCGATCTGCTTCCAGACGGCGGCGCCGGGGGTATCGGTCGCCCAGGTGGCGCGGCGCTACGCGGTGAATGC
GAACTTGATCTTCAAGTGGCTGCGCGATCGCCGTTACGCGCCGGACCCCGCCTCGGCTCTTCCCCCGTCAGAGGAGGCGCGCTTTCTGCCTGTGGAGATT
GTCGCGGAGACCAGGTCTGCCCGGGCAACACCTGCCGCCCACAACCACATCGAGATCGAATTGGCGGGCGGGCACCGGATGCGCATCAGCGGGAGCTATG
ATCCCGAGGCTCTGGCGCGGCTGATCCGGGGCGTGACGGCGTGATCCCGGTTCCGGCGAACACGCGGGTCTGGCTGGCGGCGGGCGCCACCGACATGCGC
AAGGGGTTCACGGCACTGGCGGCGCAGGCCGAGGCGGTGCTGAAGCAGGACCCGTTCGCCGGGCATCTGTTCGTCTTCCGTGGCCGTCGGGGCGATCTGG
TGAAGGTGATCTGGTGGGATGGCCAAGGCGCGTGCATGTTCATGAAGCGGCTGGAGAAGGGTCGGTTCGTCTGGCCCTCGGCCAAGGAGGGCAAGGTGGC
TTTGTCGCCTGCGCAACTGTCGATGCTGCTGGAAGGCATCGACTGGCGGGCACCGGAACGGACGTGGCGGCCTCTGGCGGCGGGATAGTCCACGGCGCCT
GCAATCAGTGATTCCCACAAGTAATACAGTGGGATAAACTTTCCGCATGCTCACCCAGGCCCTGACATTGCCGGAAGACCCCGAGGAGCTGCGCAGCTTC
ACCGCGCGGCTTCTGGCCGAGGTGAAGGCCCAGGCGATCCTGATCGAGAAGCTCCGGCATCAGCTGGCCGGGCACCGGGCGCACCGGTTTGGTGCGTCGT
CCGAGACGGCGGAACAGTTGCAGCTGGCCCTTGAGACCAGCGAGATCGCCGCCGCCGCGATGACCGCGCGGATGAAGCTGCCGGACATCGAGGAGAAGGA
CAAACCCAAGCGTCGTCCGATCCCGGACCACATCCCGCGAATGGAGGTGGAGCTGACACCGAGCACAGATGCCTGTGCCGATTGCGGCGGGCGCCTGCGC
CGGATCGGGGAAGATGTGACGGAAGAGCTGGAATACGTTCCCGGGCGGTTCATCGTGAACCGGCTTGTCCGCCCTCGGCTGACCTGCTCGTGCTGTGAAC
GCTTCGTGCAGTCCCCACTGCCCTCGCGCCCGATCGAGCGCGGTCGCCCGGGGCCGGGCCTCCTCGCCCATGTCTTGGTGAGCAAGTATGCCGACCACCT
TCCGCTCTACCGCCAGAGCCAGATCTTCGAGCGCGAAGGTCTCGACCTGGACCGGTCCACCCTGGCCGACTGGGTGGGCAAGAGCACGGCCCTGTTGGAG
CCGCTGGCCGACGCCATCGGGCGCCATGTCTTCTCAGCCGAGGCGATCTTCGCCGACGACACGCCGATCAGCATGCTGGCGCCCGGCACCGGCAAGACCC
AGACCGCCCGGCTCTGGACCTATGCGCGCGACGAACGCCCTTGGGGCGGGCAGGCCCCACCGGCGGCATGGTATCGCTTCTCCGGTGACCGCAAGGGCCA
GCATCCCAAGGACCACCTCGCCCGCTTCCGCGGCTGGATGCACGCCGACGGTTATGCCGGGTTCGAGGATCTCTACCGCTCCGGCACCATCCGCGAGGTC
GCCTGCATGGCCCATGTCCGGCGAAAGTTCGTCGATATCCATCGGTCGCAGGGCTCCCCGATCGCCGGAGAGGCCATCGGCCGGATCGCACAGCTCTACG
CCGTCGAGAAAGAAGCCCGAGGGTCGCCGCCAGACCGCCGCACGGAACTCCGCAAAGCTCACGCCGCCCCGGTCTTCGACGATCTGCAGCGATGGCTGGC
CATGAGACTGACGGAAATCTCGGGCAAATCCCCGCTCGCGGCCGCCATCCGCTATGCCCTGACCCGAATGGACCGCCTGCGCCCCTACCTCGACCACGGC
ATCCTGGAGTTGGACAACAACACCGCCGAACGCGGCATGCGCGCCATCGCCCTCGGGCGGAAGAACTACCTCTTCGTCGGCTCCGAGGCGGGCGGCAACG
CCGCTGCCATCGCCTACACCCTGATCGAAACGGCCAAGCTCAACGCCGTCGATCCCCACGCCTGGCTCGCTGACACTCTCGCCCGCATTCCCGACTACAA
GATCACCAAGGTCGATGACCTGATGCCGTGGCGCTGGAACGGATAGCGGTCACGCCGGACGGTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
396 bp	131 aa	49	444	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

VSTIDSGHCGDDVARRTKRLWTDEEKRSICFQTAAPGVSVAQVARRYAVNANLIFKWLRDRRYAPDPASALPPSEEARFLPVEIVAETRSARATPAAHNH
IEIELAGGHRMRISGSYDPEALARLIRGVTA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
348 bp	115 aa	441	788	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

VIPVPANTRVWLAAGATDMRKGFTALAAQAEAVLKQDPFAGHLFVFRGRRGDLVKVIWWDGQGACMFMKRLEKGRFVWPSAKEGKVALSPAQLSMLLEGI
DWRAPERTWRPLAAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1500 bp	498 aa	847	2346	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTQALTLPEDPEELRSFTARLLAEVKAQAILIEKLRHQLAGHRAHRFGASSETAEQLQLALETSEIAAAAMTARMKLPDIEEKDKPKRRPIPDHIPRME
VELTPSTDACADCGGRLRRIGEDVTEELEYVPGRFIVNRLVRPRLTCSCCERFVQSPLPSRPIERGRPGPGLLAHVLVSKYADHLPLYRQSQIFEREGLD
LDRSTLADWVGKSTALLEPLADAIGRHVFSAEAIFADDTPISMLAPGTGKTQTARLWTYARDERPWGGQAPPAAWYRFSGDRKGQHPKDHLARFRGWMHA
DGYAGFEDLYRSGTIREVACMAHVRRKFVDIHRSQGSPIAGEAIGRIAQLYAVEKEARGSPPDRRTELRKAHAAPVFDDLQRWLAMRLTEISGKSPLAAA
IRYALTRMDRLRPYLDHGILELNNTAERGMRAIALGRKNYLFVGSEAGGNAAAIAYTLIETAKLNAVDPHAWLADTLARIPDYKITKVDDLMPWRWNG

Blast result :

Comments

ISPye33 is 67% aa similar to ISPre3.
ISPye33 was identified by in silico sequence analysis of Paracoccus yeei strain FDAARGOS_252 Reconstructed - disrupted by insertion of ISPye32 and ISPye31.

References

1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.