ISPye25

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP020444 ND Paracoccus yeei
Paracoccus yeei
Paracoccus yeei FDAARGOS_252
DNA section
IS Length : 1452 bp

Ends


IR Length : 9

IRL : ATGCCGTAACGGGGCCTGCGTCTCGACCCGCCCGTTGCTGTTGCAAGATC
IRR : TCGGTAACGGGGTCATCCCAGTCAAGCGATTTTGCGCATACGGGATGTGC

Comments : Insertion within another IS (isoform of ISPye24) enabled precisely defining ends and DR's. IRs are not at the termini : 5 bp between IRL and left end and 3 bp for the IRR.

Insertion site


Left flankDirect repeatRight flankDR Length
agcgagaacgAGtcctgaccct2

DNA sequence

ATGCCGTAACGGGGCCTGCGTCTCGACCCGCCCGTTGCTGTTGCAAGATCGAGGGCAAGCTGACGCCATTCCCGTATTGGATCCACATGGAGGAGTGCGG
GATGAAGCTGTTTGTCGGGCTGGATGTGTCGCTGGAGAAGACCGCGATCTGCGTGATCAGCGCGCATGGGAAGATCGTGAAGGAGGCGCAGGTGGCCAGC
GAGCCCGAAGCGCTTTCGTGCTGGATCTGCGATCAGAACGGCGCAATTGCCGCCGTCGGGCTTGAGGCTGGCCCCTTGTCGCAGTGGTTGCATCGCGGGT
TGTCAGATGCGGGACTACCTGTCGTTCTGATGGAAACCAGACAGGTAAAAGGCGCCCTGAAGGCCATGCCGATCAAGACGGACCGGCGGGATGCAGAGGG
TATTGCACGTCTGCTTCATCTTGGTTGGTTCCGACCCGTTCATTGCAAATCGGTGTCTGCACAGGAGGTTAGGGCAGTGCTCAGCGCTCGTAAGGCTGTG
CAACAAGGGTTTATCACGCTGGAAATGTCACTGCGCGGTCTGCTGCGGAACTTCGGGCTCAAGGTTGGCACCATATCCCGTGGTAGGTTCGAACAGCGTA
TCCGCGAACTGGCGGCGGGCAATCTGATGCTGGAAGCGGCAACCGAACCCATGCTTCGCGCGCGCTCGGCGCTGCGGCGAGAGTTGGCGGGTCTTGAACG
CAATGTCCGTCAACTCGCCCAGGAGGATCCGGTCTGTCGTCGGCTGATGTCGATGCCCGGGATCGGCGCCGTCGTGGCGTTGACCTATCGATCTGCTGTC
GATGATCCCGCTCGCTTCACATCCTCGAAGAAGGTCGGACCGTGGGTCGGCCTGACGCCGTCTCGCAACCAGTCGGGCGAGCGCGACGTCTCGGGCGGGA
TCACCAAGGCAGGCGACGTCAACCTTCGACGCGCGCTGTGCCAGGCGGCAACCGTCATGATGCATCGTGGACGGGCGAGCTGGCTGCGAACCTGGGCGGC
AAAACTTGCGCGCCGTCGTGGTGCCAAGCGCGCGATGGTTGCGCTGGCCCGCCGCATCGCCGTGATCCTGCACCGGATGTGGAAGGATAACACCGATTTC
CGCTTTGACATTCCGGTGCTTCATGCCGGCTGAAAATACAGGTTCCAGATTGCTGCCCGCCTGATCGCGGGCCTTTGAGGTCCCCACGGGACGCGGTTCC
GGTGATGCCGTGCTCAGGACTGATGCCGATCGTATCGAGCACGCCAATGAGATGGGCACACCGGAATTGCATTGAACCAGCATCATGTTGGCGGCCACAG
CGCTGACCACGGACCGAAGCATGCACCCGGGTCGATCTCAGGCGAAGGCTGCCGAACAGGGAAGCAGATCAATTGCTCGAAGAACAAGACCGCGCTCCGG
CGGCACATCCCGTATGCGCAAAATCGCTTGACTGGGATGACCCCGTTACCGA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1032 bp343 aa1021133+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MKLFVGLDVSLEKTAICVISAHGKIVKEAQVASEPEALSCWICDQNGAIAAVGLEAGPLSQWLHRGLSDAGLPVVLMETRQVKGALKAMPIKTDRRDAEG
IARLLHLGWFRPVHCKSVSAQEVRAVLSARKAVQQGFITLEMSLRGLLRNFGLKVGTISRGRFEQRIRELAAGNLMLEAATEPMLRARSALRRELAGLER
NVRQLAQEDPVCRRLMSMPGIGAVVALTYRSAVDDPARFTSSKKVGPWVGLTPSRNQSGERDVSGGITKAGDVNLRRALCQAATVMMHRGRASWLRTWAA
KLARRRGAKRAMVALARRIAVILHRMWKDNTDFRFDIPVLHAG

 

Blast result :
Comments
ISPye25 is 73% aa similar to ISRhru3.
ISPye25 was identified by in silico sequence analysis of Paracoccus yeei strain FDAARGOS_252 (there are 3 copies od ISPye25 present in the genome, all inserted in the same position within the IR of ISPye24).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.