ISPye21
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020444 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 |
DNA section
IS Length : 1356 bp
Ends
IR Length : 12/21
IRL : TGTGACCGCCCCCCGACGGCATCTCTGTGCCAAGGTGGGTCTGGGACGAT
IRR : TGTGTAACCGCCCCAAGCGCAAGGGGGCTTTTTGGAGCTGGTGTTGGCGC
Comments : Empty insertion site in Paracoccus yeei CCUG 32053 enabled precisely defining ends and DR's.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| agatcggcgt | TGA/TTGC | agaactcccg | 6 |
DNA sequence
TGTGACCGCCCCCCGACGGCATCTCTGTGCCAAGGTGGGTCTGGGACGATCCACAAGAGGGGAGCGGTCATGTCGGAGATTAGCACGGTCGGGCTCGACC
TGGCGAAGACTGTATTTCAGGTGCACGGGGCTGACGGCTCAGGCCAGGCGGTCCTGCGAAGGAAGTTGCGGCGGCATCAGGTGCTGGCCTTCTTCGCCCA
GCTGCCATCCTGTGTCGTGGCGATGGAAGCCTGTGGTGGCGCCCATTTCTGGGGCCGAGAGATCGGGAAGCTGGGCCATGAGGTGCGGCTGATCCCTCCC
GCCTACGTCAAGCCCTTCGTCAAGCGCCAGAAGAACGATGTTGCGGATGCGGAGGCGATCTGCGAAGCCGCGCTGCGCCCGACGATGCGCTTCGTGCCGG
TAAAGAGCGAGAAGACACAGGGGGCGGCGATGGTCTTCCGCGTGCGCGAGCTTCTGATCCGGCAGCGGACGCAGGCGATCAATGCCCTGCGCGGTCACTT
GGCCGAGTTCGGGCAGATCGTGCCCCAGGGTGCGGCCAACGCCGCGCGGCTGATCGCGATCGTCGAGAACCCGGACAGCGGCCTGCCTGCCGATGCCATC
GCCACGCTGAAGGTGCTGGTCGCGGCGCTCACCCATCTCGAGACGGAGATCGGCAGGCTCGATGCCGAGATCGCCCGTCGCGCCAAGGAGAACGAGGTGG
CTCGACGCCTGATGACGGTGCCGGGCATCGGTCCGCTGATCGCCACGGCCCTCGCGGTCCTGGCCCCACCGCCCGGGACGTTCCGCAAGGCTCGGGACTT
TGCAGCCTGGCTCGGCCTCACGCCCCGGCAGCATTCGACCGGCGGCAAGCAGCGGCTCGGGGCAACGACGAAGATGGGCGAGCGATCCTTGAGACGGCTG
CTGATCCTCGGGGCGAACAGCGTCATCATCAAGCGTCACGTCCATGCCACGGCAAGGCCGGGCACCTGGCTGGCAGGAATGCTGATGCGCAAGCCACCGC
TGCTGGTGCGGGTGGCGCTGGCCAACAAGATGGCGCGGATCGTCTGGGCCTTGATGGCCCGCGGTGGCGTCTACCAGTCTCCGGCCGCGGCGGCGTAAGC
CACCGGGGTCGCGAGGGCGTCGGAGCGGAAGAGGGCAAGGAGCAGTTTGGCGCAACGGTCGTGAGACGGGGTCAGAGGAACCAGCCTGCAACAGAGTGCC
TCCGAGCACGCGGCGTTGATTTGGGACCTGATCCGCGAACACCATACGGGCCCGCGGCATGACAATGGCCGCACCAGAGGCCGGACACATGTCAGCACCC
GACACCGCGCCAACACCAGCTCCAAAAAGCCCCCTTGCGCTTGGGGCGGTTACACA
TGGCGAAGACTGTATTTCAGGTGCACGGGGCTGACGGCTCAGGCCAGGCGGTCCTGCGAAGGAAGTTGCGGCGGCATCAGGTGCTGGCCTTCTTCGCCCA
GCTGCCATCCTGTGTCGTGGCGATGGAAGCCTGTGGTGGCGCCCATTTCTGGGGCCGAGAGATCGGGAAGCTGGGCCATGAGGTGCGGCTGATCCCTCCC
GCCTACGTCAAGCCCTTCGTCAAGCGCCAGAAGAACGATGTTGCGGATGCGGAGGCGATCTGCGAAGCCGCGCTGCGCCCGACGATGCGCTTCGTGCCGG
TAAAGAGCGAGAAGACACAGGGGGCGGCGATGGTCTTCCGCGTGCGCGAGCTTCTGATCCGGCAGCGGACGCAGGCGATCAATGCCCTGCGCGGTCACTT
GGCCGAGTTCGGGCAGATCGTGCCCCAGGGTGCGGCCAACGCCGCGCGGCTGATCGCGATCGTCGAGAACCCGGACAGCGGCCTGCCTGCCGATGCCATC
GCCACGCTGAAGGTGCTGGTCGCGGCGCTCACCCATCTCGAGACGGAGATCGGCAGGCTCGATGCCGAGATCGCCCGTCGCGCCAAGGAGAACGAGGTGG
CTCGACGCCTGATGACGGTGCCGGGCATCGGTCCGCTGATCGCCACGGCCCTCGCGGTCCTGGCCCCACCGCCCGGGACGTTCCGCAAGGCTCGGGACTT
TGCAGCCTGGCTCGGCCTCACGCCCCGGCAGCATTCGACCGGCGGCAAGCAGCGGCTCGGGGCAACGACGAAGATGGGCGAGCGATCCTTGAGACGGCTG
CTGATCCTCGGGGCGAACAGCGTCATCATCAAGCGTCACGTCCATGCCACGGCAAGGCCGGGCACCTGGCTGGCAGGAATGCTGATGCGCAAGCCACCGC
TGCTGGTGCGGGTGGCGCTGGCCAACAAGATGGCGCGGATCGTCTGGGCCTTGATGGCCCGCGGTGGCGTCTACCAGTCTCCGGCCGCGGCGGCGTAAGC
CACCGGGGTCGCGAGGGCGTCGGAGCGGAAGAGGGCAAGGAGCAGTTTGGCGCAACGGTCGTGAGACGGGGTCAGAGGAACCAGCCTGCAACAGAGTGCC
TCCGAGCACGCGGCGTTGATTTGGGACCTGATCCGCGAACACCATACGGGCCCGCGGCATGACAATGGCCGCACCAGAGGCCGGACACATGTCAGCACCC
GACACCGCGCCAACACCAGCTCCAAAAAGCCCCCTTGCGCTTGGGGCGGTTACACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 70 | 1098 | + | No |
Chemistry : DEDD
ORF sequence :
MSEISTVGLDLAKTVFQVHGADGSGQAVLRRKLRRHQVLAFFAQLPSCVVAMEACGGAHFWGREIGKLGHEVRLIPPAYVKPFVKRQKNDVADAEAICEA
ALRPTMRFVPVKSEKTQGAAMVFRVRELLIRQRTQAINALRGHLAEFGQIVPQGAANAARLIAIVENPDSGLPADAIATLKVLVAALTHLETEIGRLDAE
IARRAKENEVARRLMTVPGIGPLIATALAVLAPPPGTFRKARDFAAWLGLTPRQHSTGGKQRLGATTKMGERSLRRLLILGANSVIIKRHVHATARPGTW
LAGMLMRKPPLLVRVALANKMARIVWALMARGGVYQSPAAAA
ALRPTMRFVPVKSEKTQGAAMVFRVRELLIRQRTQAINALRGHLAEFGQIVPQGAANAARLIAIVENPDSGLPADAIATLKVLVAALTHLETEIGRLDAE
IARRAKENEVARRLMTVPGIGPLIATALAVLAPPPGTFRKARDFAAWLGLTPRQHSTGGKQRLGATTKMGERSLRRLLILGANSVIIKRHVHATARPGTW
LAGMLMRKPPLLVRVALANKMARIVWALMARGGVYQSPAAAA
Blast result :
Comments
ISPye21 is 94% aa similar to ISPko7.
ISPye21 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. Reconstructed sequence - disrupted by insertion of ISPye22 (IS110 family). 2 copies of this IS are present in the genome, both inserted within the IRs of another IS (IS5 family).
ISPye21 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. Reconstructed sequence - disrupted by insertion of ISPye22 (IS110 family). 2 copies of this IS are present in the genome, both inserted within the IRs of another IS (IS5 family).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.