ISCg2
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF189147 | Y | Corynebacterium glutamicum | Corynebacterium glutamicum |
DNA section
IS Length : 1636 bp
Ends
IR Length : 20/26
IRL : GGCGGGTTCAGGGGGTGAATGCACCACGAGCAACCCGAAGGGTGCGAAGT
IRR : GGCGAATTCAATCGGTGGATGCACCAACTACGATTTTTTCATATACCTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGCGGGTTCAGGGGGTGAATGCACCACGAGCAACCCGAAGGGTGCGAAGTGGGCATTCGTAGAACAATCCCAGAGGAAAGCCGTACGGCTTTCCTCGACA
TGATCAATCAAGGTATGTCAGGTCTTGCTGCGTCTACAGCGGTCGGGGTCAGTGAATTCACCGGGCGAAAGTGGGCGAAGGCCGCCGGGGTGAAACTGAC
CCGCGGCCCGCGAGGTGGCAATGCTTTTGACACCGCCGAGAAACTTGAGATTGCAGCCAGCATGCTAGAGAAAGGATGCCTACCCCGAGAAATCGGCGAG
TATGTCGGCATGACTCGGGCCAATATATCCCTATGGCGCAAACAAGGCCCAGACAAGCTTCGCCAACGCGCAGCCACCTTGCGCACCGGCAAGCGAGCAG
CTGAATTCATCCACGCCCCGGTGATGGGCCCTTATTATGGGCCACGCACACTCCATCAAGTGTTGCGTGAGGACTACACAACACTGTTTGACGAGTTATC
TGCGTTGGGGTTGCCAGCACAGGTGTGTGGGGCCTTACTTCATCTTGCTCCACCACCATCATTACGCTTTTCTTATATGTCGTGTGTAGTGCCGTTATTT
GCTGATGAAATCAAAGTCGTAGGACAAGGCACACGATTATCGTTAGAAGAGAAAATGATGATCCAACGTTTCCATGACACCGGGGTCAGTGCAGCAGAAA
TCGGTCGACGCCTGGGTCGGTGTCGGCAAACAATTTCCAGGGAACTTCGACGTGGTCAAGATGATGATGGACGTTATCGTGCACGCGACTCCTATGAAGG
TGCGATCAGGAAACTAGCGCGTCCGAAAACACCGAAACTTGATGCCAATCGTAGGCTTCGGGCTGTGGTGGTCGAGGCGTTGAATAATAAATTATCTCCG
GAGCAGATTTCTGGTCTTTTAGCCACCGAGCATGCTAACGATAGCTCTATGCAGATTAGTCATGAAACTATTTACCAGGCGTTATATGTTCAAGGTAAAG
GGGCGTTGCGTGATGAATTGAAGGTGGAGAAATTTCTTCGTACCGGTCGGAAGGGACGTAAACCGCAGTCGAAGTTGCCATCGAGAGGTAAGCCGTGGGT
GGAGGGTGCGTTGATTAGTCAACGCCCAGCAGAAGTTGCTGATCGTGCTGTGCCTGGGCACTGGGAGGGCGATTTAGTAATTGGTGGTGAAAACCAAGCG
ACAGCGTTGGTGACGTTGGTGGAGCGCACGAGCCGGTTGACGTTGATTAAGCGGTTGGGGGTTAATCATGAGGCGTCGACTGTGACGGATGCGTTGGTGG
AGATGATGGGTGATTTGCCGCAGGCGTTGCGTCGGAGTTTGACGTGGGATCAGGGTGTGGAGATGGCAGAGCATGCGCGGTTTAGCGTGGTGACCAAGTG
TCCGGTGTTTTTCTGTGATCCTCATTCGCCGTGGCAGCGTGGGTCGAATGAGAATACGAATGGATTGGTCAGGGATTTTTTCCCGAAGGGCACTAATTTT
GCTAAAGTAAGTGACGAAGAAGTTCAGCGGGCACAGGATCTGCTGAATTACCGGCCGCGGAAAATGCATGGTTTTAAAAGCGCGACGCAGGTATATGAAA
AAATCGTAGTTGGTGCATCCACCGATTGAATTCGCC
TGATCAATCAAGGTATGTCAGGTCTTGCTGCGTCTACAGCGGTCGGGGTCAGTGAATTCACCGGGCGAAAGTGGGCGAAGGCCGCCGGGGTGAAACTGAC
CCGCGGCCCGCGAGGTGGCAATGCTTTTGACACCGCCGAGAAACTTGAGATTGCAGCCAGCATGCTAGAGAAAGGATGCCTACCCCGAGAAATCGGCGAG
TATGTCGGCATGACTCGGGCCAATATATCCCTATGGCGCAAACAAGGCCCAGACAAGCTTCGCCAACGCGCAGCCACCTTGCGCACCGGCAAGCGAGCAG
CTGAATTCATCCACGCCCCGGTGATGGGCCCTTATTATGGGCCACGCACACTCCATCAAGTGTTGCGTGAGGACTACACAACACTGTTTGACGAGTTATC
TGCGTTGGGGTTGCCAGCACAGGTGTGTGGGGCCTTACTTCATCTTGCTCCACCACCATCATTACGCTTTTCTTATATGTCGTGTGTAGTGCCGTTATTT
GCTGATGAAATCAAAGTCGTAGGACAAGGCACACGATTATCGTTAGAAGAGAAAATGATGATCCAACGTTTCCATGACACCGGGGTCAGTGCAGCAGAAA
TCGGTCGACGCCTGGGTCGGTGTCGGCAAACAATTTCCAGGGAACTTCGACGTGGTCAAGATGATGATGGACGTTATCGTGCACGCGACTCCTATGAAGG
TGCGATCAGGAAACTAGCGCGTCCGAAAACACCGAAACTTGATGCCAATCGTAGGCTTCGGGCTGTGGTGGTCGAGGCGTTGAATAATAAATTATCTCCG
GAGCAGATTTCTGGTCTTTTAGCCACCGAGCATGCTAACGATAGCTCTATGCAGATTAGTCATGAAACTATTTACCAGGCGTTATATGTTCAAGGTAAAG
GGGCGTTGCGTGATGAATTGAAGGTGGAGAAATTTCTTCGTACCGGTCGGAAGGGACGTAAACCGCAGTCGAAGTTGCCATCGAGAGGTAAGCCGTGGGT
GGAGGGTGCGTTGATTAGTCAACGCCCAGCAGAAGTTGCTGATCGTGCTGTGCCTGGGCACTGGGAGGGCGATTTAGTAATTGGTGGTGAAAACCAAGCG
ACAGCGTTGGTGACGTTGGTGGAGCGCACGAGCCGGTTGACGTTGATTAAGCGGTTGGGGGTTAATCATGAGGCGTCGACTGTGACGGATGCGTTGGTGG
AGATGATGGGTGATTTGCCGCAGGCGTTGCGTCGGAGTTTGACGTGGGATCAGGGTGTGGAGATGGCAGAGCATGCGCGGTTTAGCGTGGTGACCAAGTG
TCCGGTGTTTTTCTGTGATCCTCATTCGCCGTGGCAGCGTGGGTCGAATGAGAATACGAATGGATTGGTCAGGGATTTTTTCCCGAAGGGCACTAATTTT
GCTAAAGTAAGTGACGAAGAAGTTCAGCGGGCACAGGATCTGCTGAATTACCGGCCGCGGAAAATGCATGGTTTTAAAAGCGCGACGCAGGTATATGAAA
AAATCGTAGTTGGTGCATCCACCGATTGAATTCGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1206 bp | 401 aa | 424 | 1629 | + | No |
Chemistry : DDE
ORF sequence :
MGPYYGPRTLHQVLREDYTTLFDELSALGLPAQVCGALLHLAPPPSLRFSYMSCVVPLFADEIKVVGQGTRLSLEEKMMIQRFHDTGVSAAEIGRRLGRC
RQTISRELRRGQDDDGRYRARDSYEGAIRKLARPKTPKLDANRRLRAVVVEALNNKLSPEQISGLLATEHANDSSMQISHETIYQALYVQGKGALRDELK
VEKFLRTGRKGRKPQSKLPSRGKPWVEGALISQRPAEVADRAVPGHWEGDLVIGGENQATALVTLVERTSRLTLIKRLGVNHEASTVTDALVEMMGDLPQ
ALRRSLTWDQGVEMAEHARFSVVTKCPVFFCDPHSPWQRGSNENTNGLVRDFFPKGTNFAKVSDEEVQRAQDLLNYRPRKMHGFKSATQVYEKIVVGAST
D
RQTISRELRRGQDDDGRYRARDSYEGAIRKLARPKTPKLDANRRLRAVVVEALNNKLSPEQISGLLATEHANDSSMQISHETIYQALYVQGKGALRDELK
VEKFLRTGRKGRKPQSKLPSRGKPWVEGALISQRPAEVADRAVPGHWEGDLVIGGENQATALVTLVERTSRLTLIKRLGVNHEASTVTDALVEMMGDLPQ
ALRRSLTWDQGVEMAEHARFSVVTKCPVFFCDPHSPWQRGSNENTNGLVRDFFPKGTNFAKVSDEEVQRAQDLLNYRPRKMHGFKSATQVYEKIVVGAST
D
Blast result :
Comments
ISCg2 is 59% aa simialr to IS1513.
There are six copies of ISCg2.The six copies of ISCg2 were identical at the nucleotide level and were located in intergenic, AT-rich regions of the chromosome. The regions in which the six copies of ISCg2 were inserted displayed significant similarities. This similarity extends over a region of 65 bp, which was assumed to be the target region for ISCg2. Interestingly, five of the six copies of ISCg2 were located adjacent to genes that may be involved in aspartate and glutamate metabolism or its regulation. Investigation of the distribution of ISCg2 showed that the IS element is restricted to certain C. glutamicum strains.
There are six copies of ISCg2.The six copies of ISCg2 were identical at the nucleotide level and were located in intergenic, AT-rich regions of the chromosome. The regions in which the six copies of ISCg2 were inserted displayed significant similarities. This similarity extends over a region of 65 bp, which was assumed to be the target region for ISCg2. Interestingly, five of the six copies of ISCg2 were located adjacent to genes that may be involved in aspartate and glutamate metabolism or its regulation. Investigation of the distribution of ISCg2 showed that the IS element is restricted to certain C. glutamicum strains.
References
1] Quast,K., Bathe,B., Puhler,A. and Kalinowski,J.(1999)Mol. Gen. Genet. 262 (3), 568-578