ISPye20
- Family IS5
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020444 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 |
DNA section
IS Length : 1366 bp
Ends
IR Length : 27/36
IRL : CAGGCTGCTGAAGAACTCTGCCCTCGACGCGGTTTTCGGGACGTGATTCA
IRR : CAGGTCGCTGAAGAAGTCAGGCATCGGGACGGTTTTTCCGATCTGCCGGG
Comments : Ends based on sequence alignment with related elements (ISRosp6, ISPha2)
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| catgagagaa | ctctcatgat | 0 |
DNA sequence
CAGGCTGCTGAAGAACTCTGCCCTCGACGCGGTTTTCGGGACGTGATTCATCCTCACCGCTAGGTTTTGGGGGTGAGGGATGCGCGGGACGGACGAGGTG
ACGGGGTCGCTGTTCAGCTATGTCAACCTTGAGGAGCGGATTCCGGCCGGGCACCCGCTGCGCAAGGTGCGCGAGATCGTCAACGAAGCGCTGGCCAGCC
TCGATGCCGAGTTCGACCGGCTCTATTCGGTCGAGGGGCGCCCTTCCATCGCGCCGGAAAGGCTGCTGCGCGCGAGCCTGATCCAGATCCTGTTCTCGGT
GCGCTCCGAGCGGCAGCTGATGGAGCAGATGCGATACAACCTGCTGTTCCGGTGGTTCGTGGGCCTCGGCATCGACGACCCTGTCTGGGTGCCGACGGTG
TTCTCGAAGAACCGTGACCGCCTGCTCACGACCGACATGGCCCGGAAGTTCCTTGCGGCGATCCTCGCGCACCGCGAGGTCGCGCCGCTGCTGTCGGACG
AGCACTTCTCGGTCGACGGCACGCTGGTGAAGGCCTGGGCCTCGATGAAGAGCTTCCAGCCGATGGCCGCGGAGGCGCCGCCAGATGAGGACCGCGGCCC
GGGCGATCCGCCCAGCCCCCGCTCCGCAGACACCCCCAACCAGCCACAGCCCGAGACCGAGACCGAGACCACCCCGATGACCCGCCCCGTCCGCCAGGAT
CGTAATGCCGAAGTCGACTTCCGGGGCGAGAAGCGCTCGAACGCCACGCATGCCTCGACCACCGACCCCGAGGCGCGGCTTTACAAGAAGTCGCCTGGCG
CAGGCGCGATGCTCTGCTTTATGGGGCACACGCTGATGGAGAACCGCAGCGGTCTGATCGTCCAGGCCGACCTCACGCGTGCCGATGGCCATGCGGAGCG
CCGCGCCGCGCTCGACATGATCCACCGCCACTCGCCGGGCTCGACGCGCCGCCTGACCCTCGGCGCCGACAAGGGCTACGACAGCGCCGACTTTGTCGCC
GATCTCCGCCAAGCCTGCGTGACCCCGCATGTCGCCCGAAAGGCACGGCACTCCGCCATCGACGGACGCACCACCCGTCATCCCGGCTATTCCGCCTCGC
AGAAGCGCCGCAAGAAGATCGAGGAGCCCTTCGGCTGGGCCAAGACCGTGGGTGGCATGGCCCAGACGGTCTATCGCGGCGTCGAGCGTGTTCGCGCCAG
GTTCACCCTCACCATGGCCGCCTGCAACCTCGCCCGACTGCCGCGCCTGCTTGCCGGGTGATCGGGGGGACGAGCACGAGTGCGCCGTCCCCCCGGCCGC
AGCAGCAGTCGTCAAACCCGGCAGATCGGAAAAACCGTCCCGATGCCTGACTTCTTCAGCGACCTG
ACGGGGTCGCTGTTCAGCTATGTCAACCTTGAGGAGCGGATTCCGGCCGGGCACCCGCTGCGCAAGGTGCGCGAGATCGTCAACGAAGCGCTGGCCAGCC
TCGATGCCGAGTTCGACCGGCTCTATTCGGTCGAGGGGCGCCCTTCCATCGCGCCGGAAAGGCTGCTGCGCGCGAGCCTGATCCAGATCCTGTTCTCGGT
GCGCTCCGAGCGGCAGCTGATGGAGCAGATGCGATACAACCTGCTGTTCCGGTGGTTCGTGGGCCTCGGCATCGACGACCCTGTCTGGGTGCCGACGGTG
TTCTCGAAGAACCGTGACCGCCTGCTCACGACCGACATGGCCCGGAAGTTCCTTGCGGCGATCCTCGCGCACCGCGAGGTCGCGCCGCTGCTGTCGGACG
AGCACTTCTCGGTCGACGGCACGCTGGTGAAGGCCTGGGCCTCGATGAAGAGCTTCCAGCCGATGGCCGCGGAGGCGCCGCCAGATGAGGACCGCGGCCC
GGGCGATCCGCCCAGCCCCCGCTCCGCAGACACCCCCAACCAGCCACAGCCCGAGACCGAGACCGAGACCACCCCGATGACCCGCCCCGTCCGCCAGGAT
CGTAATGCCGAAGTCGACTTCCGGGGCGAGAAGCGCTCGAACGCCACGCATGCCTCGACCACCGACCCCGAGGCGCGGCTTTACAAGAAGTCGCCTGGCG
CAGGCGCGATGCTCTGCTTTATGGGGCACACGCTGATGGAGAACCGCAGCGGTCTGATCGTCCAGGCCGACCTCACGCGTGCCGATGGCCATGCGGAGCG
CCGCGCCGCGCTCGACATGATCCACCGCCACTCGCCGGGCTCGACGCGCCGCCTGACCCTCGGCGCCGACAAGGGCTACGACAGCGCCGACTTTGTCGCC
GATCTCCGCCAAGCCTGCGTGACCCCGCATGTCGCCCGAAAGGCACGGCACTCCGCCATCGACGGACGCACCACCCGTCATCCCGGCTATTCCGCCTCGC
AGAAGCGCCGCAAGAAGATCGAGGAGCCCTTCGGCTGGGCCAAGACCGTGGGTGGCATGGCCCAGACGGTCTATCGCGGCGTCGAGCGTGTTCGCGCCAG
GTTCACCCTCACCATGGCCGCCTGCAACCTCGCCCGACTGCCGCGCCTGCTTGCCGGGTGATCGGGGGGACGAGCACGAGTGCGCCGTCCCCCCGGCCGC
AGCAGCAGTCGTCAAACCCGGCAGATCGGAAAAACCGTCCCGATGCCTGACTTCTTCAGCGACCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1182 bp | 393 aa | 80 | 1261 | + | No |
Chemistry : DDE
ORF sequence :
MRGTDEVTGSLFSYVNLEERIPAGHPLRKVREIVNEALASLDAEFDRLYSVEGRPSIAPERLLRASLIQILFSVRSERQLMEQMRYNLLFRWFVGLGIDD
PVWVPTVFSKNRDRLLTTDMARKFLAAILAHREVAPLLSDEHFSVDGTLVKAWASMKSFQPMAAEAPPDEDRGPGDPPSPRSADTPNQPQPETETETTPM
TRPVRQDRNAEVDFRGEKRSNATHASTTDPEARLYKKSPGAGAMLCFMGHTLMENRSGLIVQADLTRADGHAERRAALDMIHRHSPGSTRRLTLGADKGY
DSADFVADLRQACVTPHVARKARHSAIDGRTTRHPGYSASQKRRKKIEEPFGWAKTVGGMAQTVYRGVERVRARFTLTMAACNLARLPRLLAG
PVWVPTVFSKNRDRLLTTDMARKFLAAILAHREVAPLLSDEHFSVDGTLVKAWASMKSFQPMAAEAPPDEDRGPGDPPSPRSADTPNQPQPETETETTPM
TRPVRQDRNAEVDFRGEKRSNATHASTTDPEARLYKKSPGAGAMLCFMGHTLMENRSGLIVQADLTRADGHAERRAALDMIHRHSPGSTRRLTLGADKGY
DSADFVADLRQACVTPHVARKARHSAIDGRTTRHPGYSASQKRRKKIEEPFGWAKTVGGMAQTVYRGVERVRARFTLTMAACNLARLPRLLAG
Blast result :
Comments
ISPye20 is 89% aa similar to ISRsp13.
ISPye20 was identified by in silico sequence analysis of Paracoccus yeei strain FDAARGOS_252.
ISPye20 was identified by in silico sequence analysis of Paracoccus yeei strain FDAARGOS_252.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.