ISPye19
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020442 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 Paracoccus yeei CCUG 32053 |
DNA section
IS Length : 1522 bp
Ends
IR Length : 9
IRL : ACCCCATGATCGCTGGGTGAGCTGACTGGGTAGGCGGCCTCTCCCGCGGC
IRR : CTCATGATCGCAGTCAACCCCTTATTCCACGACAGATATTCCGGCTCGGC
Comments : For one of the copies we identified an empty insertion sites in P. yeei TT13, which enabled precisely defining ends and DR's. The IR of this IS are not at its termini. IRL is separated 4 nt from the left-hand end of the element and 2 nt separate IRR from
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gccaaaggtt | AG | caggccgacc | 2 |
| tccggctctt | AG | caggaccccg | 2 |
| ggcggcgact | AG/A | caggctgctg | 2 |
| cgagccttag | caggtcgctg | 0 |
DNA sequence
ACCCCATGATCGCTGGGTGAGCTGACTGGGTAGGCGGCCTCTCCCGCGGCATTCTCCGTTCGAGGGTTGCACCAGATTGGCTGGTGCCGAGCCTCAAGAA
TGGGGGAGAGACCACATGGAGAGTAACACGTTTATCGGGCTGGACGTCCATAAGGCGTCGATCTCTGTCGCCGTTGCGCGTGGCGAGCGAGGCGGTGAGG
TTCGGCACTGGGGCTCCGTGGCGAACCGGCCTGATCACATCCGCAAGCTGGTGGAAAAGCTGGCCGCGAGCGGGGCGCAGCTGCGGTTTTGCTATGAGGC
TGGCCCCTGCGGCTATGGCCTTCACCGCCAGCTGGTCGAGATGGGGCATGACTGCATCGTGGTCGCGCCCTCGCTGATCCCGCTGAAGGCCGGGGACCGG
GTGAAGACGGACCGCCGCGATGCGCTGATGCTGGCCAAGCTTCACCGGGCGGGCGAGCTGACGGCGGTCTGGGTGCCGGATGCAGCCCATGAGGCGATGC
GCGATCTGATCCGGGCGCGGGCCGTGGCGATGCGGGTGACCGGCAAGGCGCGCCAGCATCTACAGGGCTTTCTGCTCCGCCACGGCCGGATCTATCCTGG
CAAGAAGGGCTGGACCGGACCCTATCGGCGCTGGCTCGCGCTGGTGCGCTTCACGCAGCCTGCGCAACAGATCGTTCTGCAGGATTACATCGACGCTGTC
GCTGATGCCGAGGCGCGGGTGGAGCGGCTGACCGGACAGATTGCGGATCTGCTGCCGCAGTGGAGCCTCGCGCCGGTGGTAGCGGCGGTTCAGGCGATGC
GCGGGGTCGGGTTCATCGTCGCGGTGACGGTCGTGGCGGAGGTCGGGGACTTCCACCGCTTCGACAGTCCGCGCCAGCTGATGGCCTATCTTGGGCTGAC
ACCGTCGGAGCATTCCACCGGGGCCAGTGTGCGCCGGGGCGGGATCACAAAGGCGGGCAGCGGCCTCGCCAGACGCGCGCTTATCGAGGGGGCCTGGAGT
TATCGGATGCAGGCGCGGATCAGCGTGAAGCTTCTGGCCCGGCTCGAGGCGCTGCCCCAGGTGGTGCGCGACATCGCCTGGAAGGGGCAACTCAGGATGT
GCCAGCGATACCGCCATCTGGTTGCAGCGGGAAAGGCGAAGGTCGTCGTCGTGACGGCGGTTGCGCGCGAGATGCTGGGCTTTGTCTGGGCGATCGCCAG
AGCGGTGACGGCGCCGCCTGTGTCGCCGGTCGCCACGGCCGTCTGAAACGGAAAGACCGCCTCGATCAGGCCGATGCGCAGAGTTGGGGGCGGAACGCGG
TGGGGAACCCGTGCCCTGTTATGGGCCGACACTGTCGATGCCCGATCCTTAGACCGAGGCAGCCCCAAGACGAAACCACGGTCATGCGGTAGCCAACCCG
CGCATGAGAGCTTGCTCAACCGTCGTCTCAGTTCCGCCTCCTGCCCTGCGCATCTATACCACAACGCACCCGGCCGAGCCGGAATATCTGTCGTGGAATA
AGGGGTTGACTGCGATCATGAG
TGGGGGAGAGACCACATGGAGAGTAACACGTTTATCGGGCTGGACGTCCATAAGGCGTCGATCTCTGTCGCCGTTGCGCGTGGCGAGCGAGGCGGTGAGG
TTCGGCACTGGGGCTCCGTGGCGAACCGGCCTGATCACATCCGCAAGCTGGTGGAAAAGCTGGCCGCGAGCGGGGCGCAGCTGCGGTTTTGCTATGAGGC
TGGCCCCTGCGGCTATGGCCTTCACCGCCAGCTGGTCGAGATGGGGCATGACTGCATCGTGGTCGCGCCCTCGCTGATCCCGCTGAAGGCCGGGGACCGG
GTGAAGACGGACCGCCGCGATGCGCTGATGCTGGCCAAGCTTCACCGGGCGGGCGAGCTGACGGCGGTCTGGGTGCCGGATGCAGCCCATGAGGCGATGC
GCGATCTGATCCGGGCGCGGGCCGTGGCGATGCGGGTGACCGGCAAGGCGCGCCAGCATCTACAGGGCTTTCTGCTCCGCCACGGCCGGATCTATCCTGG
CAAGAAGGGCTGGACCGGACCCTATCGGCGCTGGCTCGCGCTGGTGCGCTTCACGCAGCCTGCGCAACAGATCGTTCTGCAGGATTACATCGACGCTGTC
GCTGATGCCGAGGCGCGGGTGGAGCGGCTGACCGGACAGATTGCGGATCTGCTGCCGCAGTGGAGCCTCGCGCCGGTGGTAGCGGCGGTTCAGGCGATGC
GCGGGGTCGGGTTCATCGTCGCGGTGACGGTCGTGGCGGAGGTCGGGGACTTCCACCGCTTCGACAGTCCGCGCCAGCTGATGGCCTATCTTGGGCTGAC
ACCGTCGGAGCATTCCACCGGGGCCAGTGTGCGCCGGGGCGGGATCACAAAGGCGGGCAGCGGCCTCGCCAGACGCGCGCTTATCGAGGGGGCCTGGAGT
TATCGGATGCAGGCGCGGATCAGCGTGAAGCTTCTGGCCCGGCTCGAGGCGCTGCCCCAGGTGGTGCGCGACATCGCCTGGAAGGGGCAACTCAGGATGT
GCCAGCGATACCGCCATCTGGTTGCAGCGGGAAAGGCGAAGGTCGTCGTCGTGACGGCGGTTGCGCGCGAGATGCTGGGCTTTGTCTGGGCGATCGCCAG
AGCGGTGACGGCGCCGCCTGTGTCGCCGGTCGCCACGGCCGTCTGAAACGGAAAGACCGCCTCGATCAGGCCGATGCGCAGAGTTGGGGGCGGAACGCGG
TGGGGAACCCGTGCCCTGTTATGGGCCGACACTGTCGATGCCCGATCCTTAGACCGAGGCAGCCCCAAGACGAAACCACGGTCATGCGGTAGCCAACCCG
CGCATGAGAGCTTGCTCAACCGTCGTCTCAGTTCCGCCTCCTGCCCTGCGCATCTATACCACAACGCACCCGGCCGAGCCGGAATATCTGTCGTGGAATA
AGGGGTTGACTGCGATCATGAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1131 bp | 376 aa | 116 | 1246 | + | No |
Chemistry : DEDD
ORF sequence :
MESNTFIGLDVHKASISVAVARGERGGEVRHWGSVANRPDHIRKLVEKLAASGAQLRFCYEAGPCGYGLHRQLVEMGHDCIVVAPSLIPLKAGDRVKTDR
RDALMLAKLHRAGELTAVWVPDAAHEAMRDLIRARAVAMRVTGKARQHLQGFLLRHGRIYPGKKGWTGPYRRWLALVRFTQPAQQIVLQDYIDAVADAEA
RVERLTGQIADLLPQWSLAPVVAAVQAMRGVGFIVAVTVVAEVGDFHRFDSPRQLMAYLGLTPSEHSTGASVRRGGITKAGSGLARRALIEGAWSYRMQA
RISVKLLARLEALPQVVRDIAWKGQLRMCQRYRHLVAAGKAKVVVVTAVAREMLGFVWAIARAVTAPPVSPVATAV
RDALMLAKLHRAGELTAVWVPDAAHEAMRDLIRARAVAMRVTGKARQHLQGFLLRHGRIYPGKKGWTGPYRRWLALVRFTQPAQQIVLQDYIDAVADAEA
RVERLTGQIADLLPQWSLAPVVAAVQAMRGVGFIVAVTVVAEVGDFHRFDSPRQLMAYLGLTPSEHSTGASVRRGGITKAGSGLARRALIEGAWSYRMQA
RISVKLLARLEALPQVVRDIAWKGQLRMCQRYRHLVAAGKAKVVVVTAVAREMLGFVWAIARAVTAPPVSPVATAV
Blast result :
Comments
ISPye19 is 94% aa similar to ISPye17.
ISPye19 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strains: CCUG 32053 (2 copies) and FDAARGOS_252 (2 isoforms).
ISPye19 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strains: CCUG 32053 (2 copies) and FDAARGOS_252 (2 isoforms).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.