ISFsi3
- Family ISNCY
- Group ISFsi2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_015672 | ND | Flexistipes sinusarabici | Flexistipes sinusarabici DSM 4947 |
DNA section
IS Length : 1473 bp
Ends
IR Length : 17/18
IRL : CTGGCGTCACCCCATTTTATTATAAAAGATATTGTATGACATGTATTTAG
IRR : CTAGCGTCACCCCATTTTTCGCAGACTTATGCCACGAGTAAACTTTTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGTGATGCC | AGCTAAAATA | TGATTGATTA | 10 |
| ATGTTAACCT | ATATTAAAAAA | TGGTTGACAA | 11 |
| GGAGGGTGCC | AATAATAAAA | GCATTGCAAT | 10 |
| AAAGAGGAAT | AATATTTA | CTATTGCAAA | 8 |
| AATATGAAAA | AATTGTTTCT | CTATTGAACT | 10 |
| CCAAATGGGG | TAATTTTA | TCATTGCTTC | 8 |
DNA sequence
CTGGCGTCACCCCATTTTATTATAAAAGATATTGTATGACATGTATTTAGAGTTGAGAAGTCCTCCAGGATCGTGTATAGTTATTTTCGAGAAAAAAGCA
ACAAATACAACGACCTGGAGGAATTTTATGATAAGTGAAACCGTGGAAAATGTGAAGGGAAAAATTAGAAAAATTGTGCACGATCTCAACGAACATATTT
CAAAGCCTCAGCAAAAATATTTACTGGAAATGATTCCGGGATGTTTTTCAACAGGCAGCTTAAATCTTACATCCATATCGGGCTATCTGAGTGAAAAGAC
CAAAGTAAAACATACGCTAAAAAGGCTGCAAAGGAATACAGAGAACTATTCGTCCCTGCTTAAAATTTCAAATTTATATAATATTCACAGCAGTTATGAA
GAAACGATAAAAGATGAGAGAGTACTAATATCAGTGGATGAGGGAGATCTGGTTCATGATTATGGCAAGTCATTTGAACTCATATCGAAGGTTCGTGACG
GCAGCAGCAAAAAGAAACGGATAAATAATGGTTATTTTCTGAATCATGCAGTTTGTTACAGTCTTTCCAGCAAAAGAGTGTTACCGCTTTATCTTGATAT
TCACAGTAGCATTTCCCCTGATTTCAAGAGTGCCAACAATGAGACAATAAAATTATTGGATACAATAGAGAAAAAATTCAAAGATAAGGGTATTTTTGTA
ATGGACAGGGGCTATGATGCTGGAGTTATACTGGAATATCTTTATAAAAAGGGTCTGAGTTTTATAATAAGAAGCGTTGGTAACCGTCATGTAACTCACA
GAGGCAAGAACGTACCGGTTTCCAAGTTGTGCAAATCTGTCATAAACAAACGTTACAAAAAGAATAGTTTTTCTTATGGTTATGCGAAATGTTATTATAA
GGGGCGTCCTATGACGGTAATAAGTGCTAAGGGGGCAGAAAAGGATAATTATGTCTATCTTCTTTGCGAGGGTCATATAAGAAAGAGCAAGGAAGCCTTT
TTCCGGGTGAAGAGTTATTTCAAAAGATGGAAGGTAGAAGAGAGTTTCAGATTTATGAAGCAGCAGTTGGGCATAGAGAGATGTCTTGTGAGGAAATTTG
ATTCTATAAAGACAATGCTTGGTATAGCTTCTTTTTGCTGGAATTTATTATCCCGGATAGAATCAGACAGATTGTTAGCGGTGGAACTTGAGAGAATGTC
GAGACGTGAGAAATATAACACAAAAAATAGGACAGTGTGCACATTTATGCATTACAGGATATCAGACGGTATCAGGAATATGTTATTGTCTTATAATAAG
AGGCTTTTTAGATTTAGAGATAAAAAATATAAATCAGATATAGTGTATTATATGAAGATACCGTATTATTTAGAAAAACATAGGGAAAGAGAAATTATAG
ATGGTATTCCTGTTGTCAGAAGGAAAAAAAGTTTACTCGTGGCATAAGTCTGCGAAAAATGGGGTGACGCTAG
ACAAATACAACGACCTGGAGGAATTTTATGATAAGTGAAACCGTGGAAAATGTGAAGGGAAAAATTAGAAAAATTGTGCACGATCTCAACGAACATATTT
CAAAGCCTCAGCAAAAATATTTACTGGAAATGATTCCGGGATGTTTTTCAACAGGCAGCTTAAATCTTACATCCATATCGGGCTATCTGAGTGAAAAGAC
CAAAGTAAAACATACGCTAAAAAGGCTGCAAAGGAATACAGAGAACTATTCGTCCCTGCTTAAAATTTCAAATTTATATAATATTCACAGCAGTTATGAA
GAAACGATAAAAGATGAGAGAGTACTAATATCAGTGGATGAGGGAGATCTGGTTCATGATTATGGCAAGTCATTTGAACTCATATCGAAGGTTCGTGACG
GCAGCAGCAAAAAGAAACGGATAAATAATGGTTATTTTCTGAATCATGCAGTTTGTTACAGTCTTTCCAGCAAAAGAGTGTTACCGCTTTATCTTGATAT
TCACAGTAGCATTTCCCCTGATTTCAAGAGTGCCAACAATGAGACAATAAAATTATTGGATACAATAGAGAAAAAATTCAAAGATAAGGGTATTTTTGTA
ATGGACAGGGGCTATGATGCTGGAGTTATACTGGAATATCTTTATAAAAAGGGTCTGAGTTTTATAATAAGAAGCGTTGGTAACCGTCATGTAACTCACA
GAGGCAAGAACGTACCGGTTTCCAAGTTGTGCAAATCTGTCATAAACAAACGTTACAAAAAGAATAGTTTTTCTTATGGTTATGCGAAATGTTATTATAA
GGGGCGTCCTATGACGGTAATAAGTGCTAAGGGGGCAGAAAAGGATAATTATGTCTATCTTCTTTGCGAGGGTCATATAAGAAAGAGCAAGGAAGCCTTT
TTCCGGGTGAAGAGTTATTTCAAAAGATGGAAGGTAGAAGAGAGTTTCAGATTTATGAAGCAGCAGTTGGGCATAGAGAGATGTCTTGTGAGGAAATTTG
ATTCTATAAAGACAATGCTTGGTATAGCTTCTTTTTGCTGGAATTTATTATCCCGGATAGAATCAGACAGATTGTTAGCGGTGGAACTTGAGAGAATGTC
GAGACGTGAGAAATATAACACAAAAAATAGGACAGTGTGCACATTTATGCATTACAGGATATCAGACGGTATCAGGAATATGTTATTGTCTTATAATAAG
AGGCTTTTTAGATTTAGAGATAAAAAATATAAATCAGATATAGTGTATTATATGAAGATACCGTATTATTTAGAAAAACATAGGGAAAGAGAAATTATAG
ATGGTATTCCTGTTGTCAGAAGGAAAAAAAGTTTACTCGTGGCATAAGTCTGCGAAAAATGGGGTGACGCTAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1320 bp | 439 aa | 128 | 1447 | + | No |
Chemistry : Unknow
ORF sequence :
MISETVENVKGKIRKIVHDLNEHISKPQQKYLLEMIPGCFSTGSLNLTSISGYLSEKTKVKHTLKRLQRNTENYSSLLKISNLYNIHSSYEETIKDERVL
ISVDEGDLVHDYGKSFELISKVRDGSSKKKRINNGYFLNHAVCYSLSSKRVLPLYLDIHSSISPDFKSANNETIKLLDTIEKKFKDKGIFVMDRGYDAGV
ILEYLYKKGLSFIIRSVGNRHVTHRGKNVPVSKLCKSVINKRYKKNSFSYGYAKCYYKGRPMTVISAKGAEKDNYVYLLCEGHIRKSKEAFFRVKSYFKR
WKVEESFRFMKQQLGIERCLVRKFDSIKTMLGIASFCWNLLSRIESDRLLAVELERMSRREKYNTKNRTVCTFMHYRISDGIRNMLLSYNKRLFRFRDKK
YKSDIVYYMKIPYYLEKHREREIIDGIPVVRRKKSLLVA
ISVDEGDLVHDYGKSFELISKVRDGSSKKKRINNGYFLNHAVCYSLSSKRVLPLYLDIHSSISPDFKSANNETIKLLDTIEKKFKDKGIFVMDRGYDAGV
ILEYLYKKGLSFIIRSVGNRHVTHRGKNVPVSKLCKSVINKRYKKNSFSYGYAKCYYKGRPMTVISAKGAEKDNYVYLLCEGHIRKSKEAFFRVKSYFKR
WKVEESFRFMKQQLGIERCLVRKFDSIKTMLGIASFCWNLLSRIESDRLLAVELERMSRREKYNTKNRTVCTFMHYRISDGIRNMLLSYNKRLFRFRDKK
YKSDIVYYMKIPYYLEKHREREIIDGIPVVRRKKSLLVA
Blast result :
Comments
ISFsi3 is 59% aa similar to ISFsi2.
References
1] ISfinder annotation (2013)
2] Lucas,S., Han,J., Lapidus,A., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Chertkov,O., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Schroeder,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. (2011) Direct submission GenBank.
2] Lucas,S., Han,J., Lapidus,A., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Chertkov,O., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Schroeder,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. (2011) Direct submission GenBank.