ISFsi2
- Family ISNCY
- Group ISFsi2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_015672 | ND | Flexistipes sinusarabici | Flexistipes sinusarabici DSM 4947 |
DNA section
IS Length : 1435 bp
Ends
IR Length : 18
IRL : TTGGCACCCTCCCATTTTCTCAAAAAGATATTTGATAAGTATTTGTAAAG
IRR : TTGGCACCCTCCCATTTTTTTCACAGTATCATACCAAGTGAACGTTTATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTCAGCTTT | TTGTTTTTTG | 0 | |
| TCATATTAAT | TTTTACAAAA | AACTTTTTAT | 10 |
| TATTTTTCCA | TATTTTTCAG | 0 | |
| CAGTAATATT | TTGTTTTTTG | 0 | |
| ATTACTTACT | TAATAAAAC | TGGCGTCACC | 9 |
| TCCGGCTGAT | AACGATATT | TTGTTGACAA | 9 |
| TTTGATATAG | AAAAGCAAC | ATATTGTTTT | 9 |
DNA sequence
TTGGCACCCTCCCATTTTCTCAAAAAGATATTTGATAAGTATTTGTAAAGAAAGGTAAAAGATGAAGATAAGTGATTGAATAAGCCTCCTTTTTCCTGTA
TAGTGAATTTACCATAAAGCACTAACAAAAAAGAAAAAGGAGGCAGTTTATGATAGGTGAAATTAGGAAAAATGTGAAGAGAAAAATTGTGAGTGTAGCA
TCATCAATTTCAGAGGATAATGTAAGTGTAAAAAAGCCCCTTCATAAACATATTTTGGAAATAGTAACCGGAGTATTAGCCTCAAAGAGCTGCAACTTAA
CAGAAATAGCAAGAAGCCTGAAGGAAGATATAGCCATAAAAGACACTTTAAAAAGGATACGCCGTAATGTCCATGATCACCCAGAGATTCTTGAATTGTC
GAATTATTACAATATGAACAAGTGGAAAGACAAAGTAAGGGAAGAAACAATAATAGCTTTGGATGCGGGAGACATTTGTCATCATTTTGGCAATAACTTT
GAAAATCATTGTCGTTTAAGAGATGGGAGCAAAGGCAGTACCGGCAACGGTTATTATCTAAATCAAATAAGTTGTTACAATCCTTCTGAGAGAATAACAT
TTCCAATGTATCTTGATATGTACAGCAGCGAAGAACAGAGCTTTAAGAGTATGAACACGGAAAGTATGAAAGCGGTAGAGAATTTTGTATCAGCAGCTGG
ACCCAAAGGTTTGTGGGTATTAGACCGTGGTTATGACGGCGGTATTATGCTGAATTATTTTTTAAACAAGGATCTTGATTTTGTAACCAGATTAACAAAA
AAGCGCCACCTTGTTTTAGGAGGTAAACCGGTAAGTATTCCTGATTTAGTGAAGAAAATAAACAGACGTTACAAAATTGGGAAATCTTTTAGGTTTGGCT
ATAAGAAGTGTTATATAAATCTTGAAGGTAAGCTTTACCCTGTGACTGTAATGGTTAATAAGGGGGAGGAGAATAAAGAGCCTCATATTTTACTTACAAA
TGGTCACATAAAGAAATCCAGAGAGATTAAGAGACGCGTGACAGGATATTACCACCGCTGGGGCGTGGAAGAATGTTACAGATTTGAGAAGCAGGGCTTT
GGAATAGAGAAGAGTTTAACCCCTAACTTTAATGCGATAAAATCACTGCTTGGAGCATCCATGTTAGCCTGGAGCGTATTGCTGATGGTACAGGAGGATG
AAATATTAAAAGAGCAGGTCATTGCAAATTCTAAGAGGGAGAAATCCAAAAAGAAAGATCGACCAAAATTTATATATTATAGCCTTTTAGATGGTATTAG
CAGAGCATTTATTATGGCTAAGGAAATATTTAGATTTCGAAAACCCAAGCCGCCTAATTTAGCTCCTACAATTGATGAATTGTTAAATAAACGTTCACTT
GGTATGATACTGTGAAAAAAATGGGAGGGTGCCAA
TAGTGAATTTACCATAAAGCACTAACAAAAAAGAAAAAGGAGGCAGTTTATGATAGGTGAAATTAGGAAAAATGTGAAGAGAAAAATTGTGAGTGTAGCA
TCATCAATTTCAGAGGATAATGTAAGTGTAAAAAAGCCCCTTCATAAACATATTTTGGAAATAGTAACCGGAGTATTAGCCTCAAAGAGCTGCAACTTAA
CAGAAATAGCAAGAAGCCTGAAGGAAGATATAGCCATAAAAGACACTTTAAAAAGGATACGCCGTAATGTCCATGATCACCCAGAGATTCTTGAATTGTC
GAATTATTACAATATGAACAAGTGGAAAGACAAAGTAAGGGAAGAAACAATAATAGCTTTGGATGCGGGAGACATTTGTCATCATTTTGGCAATAACTTT
GAAAATCATTGTCGTTTAAGAGATGGGAGCAAAGGCAGTACCGGCAACGGTTATTATCTAAATCAAATAAGTTGTTACAATCCTTCTGAGAGAATAACAT
TTCCAATGTATCTTGATATGTACAGCAGCGAAGAACAGAGCTTTAAGAGTATGAACACGGAAAGTATGAAAGCGGTAGAGAATTTTGTATCAGCAGCTGG
ACCCAAAGGTTTGTGGGTATTAGACCGTGGTTATGACGGCGGTATTATGCTGAATTATTTTTTAAACAAGGATCTTGATTTTGTAACCAGATTAACAAAA
AAGCGCCACCTTGTTTTAGGAGGTAAACCGGTAAGTATTCCTGATTTAGTGAAGAAAATAAACAGACGTTACAAAATTGGGAAATCTTTTAGGTTTGGCT
ATAAGAAGTGTTATATAAATCTTGAAGGTAAGCTTTACCCTGTGACTGTAATGGTTAATAAGGGGGAGGAGAATAAAGAGCCTCATATTTTACTTACAAA
TGGTCACATAAAGAAATCCAGAGAGATTAAGAGACGCGTGACAGGATATTACCACCGCTGGGGCGTGGAAGAATGTTACAGATTTGAGAAGCAGGGCTTT
GGAATAGAGAAGAGTTTAACCCCTAACTTTAATGCGATAAAATCACTGCTTGGAGCATCCATGTTAGCCTGGAGCGTATTGCTGATGGTACAGGAGGATG
AAATATTAAAAGAGCAGGTCATTGCAAATTCTAAGAGGGAGAAATCCAAAAAGAAAGATCGACCAAAATTTATATATTATAGCCTTTTAGATGGTATTAG
CAGAGCATTTATTATGGCTAAGGAAATATTTAGATTTCGAAAACCCAAGCCGCCTAATTTAGCTCCTACAATTGATGAATTGTTAAATAAACGTTCACTT
GGTATGATACTGTGAAAAAAATGGGAGGGTGCCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1266 bp | 421 aa | 150 | 1415 | + | No |
Chemistry : Unknow
ORF sequence :
MIGEIRKNVKRKIVSVASSISEDNVSVKKPLHKHILEIVTGVLASKSCNLTEIARSLKEDIAIKDTLKRIRRNVHDHPEILELSNYYNMNKWKDKVREET
IIALDAGDICHHFGNNFENHCRLRDGSKGSTGNGYYLNQISCYNPSERITFPMYLDMYSSEEQSFKSMNTESMKAVENFVSAAGPKGLWVLDRGYDGGIM
LNYFLNKDLDFVTRLTKKRHLVLGGKPVSIPDLVKKINRRYKIGKSFRFGYKKCYINLEGKLYPVTVMVNKGEENKEPHILLTNGHIKKSREIKRRVTGY
YHRWGVEECYRFEKQGFGIEKSLTPNFNAIKSLLGASMLAWSVLLMVQEDEILKEQVIANSKREKSKKKDRPKFIYYSLLDGISRAFIMAKEIFRFRKPK
PPNLAPTIDELLNKRSLGMIL
IIALDAGDICHHFGNNFENHCRLRDGSKGSTGNGYYLNQISCYNPSERITFPMYLDMYSSEEQSFKSMNTESMKAVENFVSAAGPKGLWVLDRGYDGGIM
LNYFLNKDLDFVTRLTKKRHLVLGGKPVSIPDLVKKINRRYKIGKSFRFGYKKCYINLEGKLYPVTVMVNKGEENKEPHILLTNGHIKKSREIKRRVTGY
YHRWGVEECYRFEKQGFGIEKSLTPNFNAIKSLLGASMLAWSVLLMVQEDEILKEQVIANSKREKSKKKDRPKFIYYSLLDGISRAFIMAKEIFRFRKPK
PPNLAPTIDELLNKRSLGMIL
Blast result :
Comments
ISFsi2 is 60% aa similar to ISFsi3.
References
1] ISfinder annotation (2013)
2] Lucas,S., Han,J., Lapidus,A., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Chertkov,O., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Schroeder,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. (2011) Direct submission GenBank.
2] Lucas,S., Han,J., Lapidus,A., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Chertkov,O., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Schroeder,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. (2011) Direct submission GenBank.