ISfinder Mirror Site

ISPye15

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP020442	ND	Paracoccus yeei	Paracoccus yeei Paracoccus yeei FDAARGOS_252

DNA section

IS Length : 1421 bp

Ends

IR Length : 13/13

IRL : CATTACAGTTGCATTCTTTTCTCCGCTCTGAATCAATGCGCTACCATGAC
IRR : CATTACAGTTGCACGTTTCTTCGAAGGTGTGCCTGCCTTGCTGCGGCTTG

Comments : For some of the copies we identified empty insertion sites in other strains, which enabled precisely defining ends and DR's.

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCGCTATTGC	CTAG	CTTGAGCGCC	4
CGCATCCCTG	CTAG/T	GTTCCCAGCG	4
ATGGCGTGAC	CTAG	GGACGCTGAG	4
CACCCGCGAA	CTAA	GCCGGGGTGA	4

DNA sequence

CATTACAGTTGCATTCTTTTCTCCGCTCTGAATCAATGCGCTACCATGACTAAGTTAGGGATCATGGATGGCGGGGTTGTCGGTCGAAGAGACGCTGGAG
CTTTGGGCTTCCTCACTCCGTGAAGTAAAGGCTCGAATTCGGCCGCTGTTTACGCAGGAACGGGTTGCGGCATCGGCCGGTCAGTTTCTCGACGGCTTGT
TGGGTGAGGAACGGCGCAAGACGGGTTGGATGCGGGCCGAAGCGGCTGGAGATCCGGGCCCCTGGCGTCAGCAGGCGATATTGGGTCGTGGCCATTGGGA
TGCCGACGCGCTGCGCGATATTGTGCGGGACTACGCCGTGGAAACGCTGGCTGATCCCGAGGCGGTGCTGGTGATCGATGAAACCGGTTTTTTGAAGCAG
GGCCACGCCTCCTGTGGGGTCGCGCGCCAATATACGGGGTCGGCAGGAAAGATCACCAATTGCCAGATCGGCGTCTTCGCCAGCTACGTCTCGCAGCACG
GACATGCTTTTATCGATCGGGCCCTCTATCTGCCGAAAGCATGGGCTGAAGCTCCGGTCAGACGAAGCCGGGCCCATGTGCCCGAGGCGGTGGAGTTCGC
CACTAAACCTGCCATCGCCCGCAGCATGATCGAGCGCGTGCTCTCGACCAAGGTTCCATTTTCCTGGGTGGCCGCCGACAGCGTCTATGGCGTTGGCGAT
ATCGAAATAGCACTGCGCCGCGCTGGAAAAGGCTATGTCCTGGGTGTCAGTTCCAAGCACGCCTTCAATTCCTGGGGGAAAGCCTATGCCGTCAGCGGGA
CGGCCGGGAGCATCGCGGAAGACTTGCCGCCCGAGGCTTGGCAGCGTCTTTCGGCCGGCGCGGGCACCAAGGGCGAGCGGCTTCACGACTGGGCCTACCT
TGATCTCGCCGATCTCGATGCTGGCGAATTCAATGCTGATCTCAGCGGCGTATGGACCCGGGGCCTGCTGATCCGACGCAATATCTCCGATGGCGAGTTG
GCGTTCTTCACCACATGGTGTCCGGAGGGCACGGGCATGGAAACACTGGTGCGTGTCGAAGGACATCGCTGGGCAATCGAGGACAGCTTCGAGACCGCAA
AGAATGAACTCGGCCTCGATCACAACGAGACCCGTTCCTGGCACGGGTGGCACCGCCATGTATCGCTGGTCATGCTGGCCTTCGCCATGATGGCTACCAT
TCGCCATCACGCCAATGTCGAACCGCCCAAAAAAACGATGCGCAGGCTGCCAAGGAACCCAAGCTGATCCGCTGGTCAATTCAGGAGATCCGACGCATCG
CCAATCGGCTGGCCCAACGTCAGATTGAACCGGCTTATATTATCGCTTGGTCGATGTGGCGAAGGGCTCATCAAGCCGCAGCAAGGCAGGCACACCTTCG
AAGAAACGTGCAACTGTAATG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1200 bp	399 aa	68	1267	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAGLSVEETLELWASSLREVKARIRPLFTQERVAASAGQFLDGLLGEERRKTGWMRAEAAGDPGPWRQQAILGRGHWDADALRDIVRDYAVETLADPEAV
LVIDETGFLKQGHASCGVARQYTGSAGKITNCQIGVFASYVSQHGHAFIDRALYLPKAWAEAPVRRSRAHVPEAVEFATKPAIARSMIERVLSTKVPFSW
VAADSVYGVGDIEIALRRAGKGYVLGVSSKHAFNSWGKAYAVSGTAGSIAEDLPPEAWQRLSAGAGTKGERLHDWAYLDLADLDAGEFNADLSGVWTRGL
LIRRNISDGELAFFTTWCPEGTGMETLVRVEGHRWAIEDSFETAKNELGLDHNETRSWHGWHRHVSLVMLAFAMMATIRHHANVEPPKKTMRRLPRNPS

Blast result :

Comments

ISPye15 is 84% aa similar to ISRm31.
ISPye15 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252 (4 copies of this IS present in the genome).

References

1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.